General Information

Product

MAP3K14 Antibody Affinity Purified

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 14 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-350 on human MAP3K14 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q99558

Overview

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Alternative Nomenclature

MAP3K14 antibody

FTDCR1B antibody

HS antibody

HsNIK antibody

Mitogen activated protein kinase kinase kinase 14 antibody

NF kappa beta inducing kinase antibody

NIK antibody

Serine/threonine-protein kinase NIK antibody

General Information

Product

MAPK14 Antibody

Description

Affinity Purified p38 Mitogen Activated Protein Kinase 14 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human Mitogen Activated Protein Kinase 14 protein.

Accession #

GenBank: ACI00233.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q16539

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation.

Molecular Function

ATP binding; MAP kinase activity; MAP kinase kinase activity; NFAT protein binding; Protein serine/threonine kinase activity

Subcellular Location

Cytoplasm; Nucleus

Structure

Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B.

Alternative Nomenclature

CSAID-binding protein antibody

CSBP antibody

Cytokine suppressive anti-inflammatory drug-binding protein antibody

EXIP antibody

MAP kinase 14 antibody

MAP kinase p38 alpha antibody

MAPK14 antibody

MAX-interacting protein 2 antibody

Mitogen-activated protein kinase 14 antibody

p38 antibody

p38 MAP kinase antibody

SAPK2A antibody

Stress-activated protein kinase 2a antibody

General Information

Product

Phospho MAPK14 Antibody Affinity Purified

Description

Affinity Purified Phospho p38 Mitogen Activated Protein Kinase 14 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human MAPK14 protein.

Accession #

NCBI:  NP_001306

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q16539

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation.

Molecular Function

ATP binding; MAP kinase activity; MAP kinase kinase activity; NFAT protein binding; Protein serine/threonine kinase activity

Subcellular Location

Cytoplasm; Nucleus

Expression

Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

Structure

Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B.

Alternative Nomenclature

CSAID-binding protein antibody

CSBP1 antibody

CSBP2 antibody

Cytokine suppressive anti-inflammatory drug-binding protein antibody

EXIP antibody

MAP kinase 14 antibody

MAP kinase MXI2 antibody

MAP kinase p38 alpha antibody

MAPK14 antibody

MAX-interacting protein 2 antibody

Mitogen-activated protein kinase 14 antibody

Mitogen-activated protein kinase p38 alpha antibody

Mxi2 antibody

p38 antibody

p38 MAP kinase antibody

p38 mitogen activated protein kinase antibody

p38alpha Exip antibody

PRKM14 antibody

PRKM15 antibody

RK antibody

SAPK2A antibody

Stress-activated protein kinase 2a antibody

General Information

Product

CAMKII gamma Antibody

Description

Affinity Purified Calcium/calmodulin-dependent protein kinase type II subunit gamma Antibody

Verified Applications

CM, DB, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 400-455 on human CAMKII gamma protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.8 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13555

Overview

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in sarcoplsamic reticulum Ca2+ transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.

Molecular Function

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

Expression

Skeletal muscle

Structure

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.

Alternative Nomenclature

Calcium/calmodulin dependent protein kinase type 1G antibody

CaM kinase I gamma antibody

CaM kinase IG antibody

CaMK-like CREB kinase III antibody

Camk1g antibody

CLICK III antibody

VWS1 antibody

General Information

Product

CAMKII gamma Antibody

Description

Affinity Purified Calcium/calmodulin-dependent protein kinase type II subunit gamma isoform 3 Antibody N-epitope

Verified Applications

CM, DB, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human CAMKII gamma protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.8 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13555

Overview

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in sarcoplsamic reticulum Ca2+ transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.

Molecular Function

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

Expression

Skeletal muscle

Structure

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of t

Alternative Nomenclature

Calcium/calmodulin dependent protein kinase type 1G antibody

CaM kinase I gamma antibody

CaM kinase IG antibody

CaMK-like CREB kinase III antibody

Camk1g antibody

CLICK III antibody

VWS1 antibody

General Information

Product

Punt Antibody

Description

Affinity Purified Punt receptor Serine/Threonine kinase (punt) Antibody

Verified Applications

ELISA, ICC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Synthetic peptide corresponding to C terminal amino acids 497-515 of Drosophila melanogaster PUNT protein. Peptides were amidated for conjugation.

Sequence:

QLLIKNHTNIDDAKESTNC

Accession #

GenBank: L38495.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q24468

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Activin receptor complex, Plasma membrane

Structure

Contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 88CD antibody
Activin receptor type II antibody
Atr antibody
Atr88CD antibody
AtrII antibody
dlhC antibody
Dorsal holes C antibody
j5A5 antibody
Pun antibody
Put antibody
STKC antibody
TGF beta l antibody
TGF beta type receptor like antibody
Tgf r antibody

General Information

Product

Punt Antibody

Description

Affinity Purified Punt receptor Serine/Threonine kinase (punt) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Punt protein.

Accession #

GenBank: L38495.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q24468

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Activin receptor complex, Plasma membrane

Structure

Contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 88CD antibody
Activin receptor type II antibody
Atr antibody
Atr88CD antibody
AtrII antibody
dlhC antibody
Dorsal holes C antibody
j5A5 antibody
Pun antibody
Put antibody
STKC antibody
TGF beta l antibody
TGF beta type receptor like antibody
Tgf r antibody

General Information

Product

RIPK3 Antibody 

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 3 Antibody Extreme C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Pig, Monkey

Immunogen

Synthetic peptide taken within amino acid region 468-518 on human Receptor-interacting Serine/Threonine-protein kinase 3 protein.

Accession #

NCBI:  NP_006862.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y572

Overview

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Cell membrane, Mitochondrion

Expression

Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.

Structure

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative Nomenclature

Receptor interacting protein 3 antibody

Receptor interacting serine threonine kinase 3 antibody

Receptor-interacting protein 3 antibody

RIP 3 antibody

RIP like protein kinase 3 antibody

RIPK 3 antibody

General Information

Product

RIPK3 Antibody Affinity Purified

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 3 Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Pig, Monkey

Immunogen

Synthetic peptide taken within amino acid region 461-511 on human Receptor-interacting Serine/Threonine-protein kinase 3 protein.

Accession #

NCBI:  NP_006862.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y572

Overview

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Cell membrane, Mitochondrion

Expression

Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.

Structure

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative Nomenclature

Receptor interacting protein 3 antibody

Receptor interacting serine threonine kinase 3 antibody

Receptor-interacting protein 3 antibody

RIP 3 antibody

RIP like protein kinase 3 antibody

RIPK 3 antibody

General Information

Product

Saxophone Antibody Affinity Purified

Description

Affinity Purified Serine/Threonine kinase receptor SAX (sax) mRNA, complete cds Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequence near C-terminal of Saxophone protein.

Accession #

GenBank: U11441.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q7JPM7

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase

Alternative Nomenclature

CG1891 antibody

receptor protein serine/threonine kinase antibody

saxophone antibody

X linked surface antigen antibody