General Information |
|
Product |
VEGFR1 Antibody FITC-Conjugated |
Description |
FITC-Conjugated Vascular Endothelial Growth Factor Receptor 1 Antibody |
Verified Applications |
ELISA, IHC, IP, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Monkey |
Immunogen |
Synthetic peptide taken within amino acid region 1250-1300 on human Vascular endothelial growth factor receptor 1 protein. |
|
|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.5-1.5 µg/µl in antibody stabilization buffer |
Immunoglobulin |
IgG |
Form |
FITC-Conjugated |
Clonality |
Polyclonal |
Storage |
-20⁰C for long term storage |
|
|
ELISA |
1:50,000 |
Immunohistochemistry | 1:100 |
Immunoprecipitation |
1:200 |
Western Blot |
1:2,500-1:5,000 |
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|
Uniprot # |
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Overview |
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion. |
Molecular Function |
Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase |
Subcellular Location |
Isoform 1 :Cell membrane; Single-pass type I membrane protein Endosome Note: Autophosphorylation promotes ubiquitination and endocytosis. Isoform 2 :Secreted 1 Publication Isoform 3 :Secreted Isoform 4 :Secreted Isoform 5 :Cytoplasm Curated Isoform 6 :Cytoplasm Curated Isoform 7 :Cytoplasm Curated |
Expression |
Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC). |
Structure |
The second and third Ig-like C2-type (immunoglobulin-like) domains are sufficient for VEGFA binding. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 7 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain. |
Alternative Nomenclature |
EC 2.7.10.1 antibody FLT antibody FLT1 antibody Fms like tyrosine kinase 1 antibody Fms related tyrosine kinase 1 antibody Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody FRT antibody Soluble VEGF receptor 1 14 antibody Soluble VEGFR1 variant 2 antibody Soluble VEGFR1 variant 21 antibody Tyrosine protein kinase FRT antibody Tyrosine protein kinase receptor FLT antibody Vascular endothelial growth factor vascular permeability factor receptor antibody Vascular permeability factor receptor 1 antibody VEGFR1 antibody |
Protein information supplied by UniProt
Product | Note | Status | Price | |
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VEGFR1-101AP | |||
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P-VEGFR1 | |||
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VEGFR1-BIOTIN | |||
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PC-VEGFR1 | |||
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