PDE4A Antibody

Product no.: PD4A-112AP

General Information
Product	PDE4A Antibody
Description	Affinity Purified Phosphodiesterase 4A Antibody
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Moose, Mouse, Rat
Immunogen	Synthetic cyclic peptide corresponding to the C-terminal region of PDE4A. Common to all PDE4A proteins.
Specificity	This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), PDE4A5 (109 kDa), PDE4A8 (106 kDa), AND PDE4Ax (10 kDa) and a 76 kDa testis specific A variant.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:100-1:400
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:100-1:400
Immunofluorescence	1:100-1:400
Immunohistochemistry	1:100-1:400
Immunoprecipitation	1:250
Western Blot	1:1,000-1:2,500
Protein
Uniprot #	P27815
Overview	Hydrolyzes the second messenger cAMP which is a key regulator of many important physiological processes.
Molecular Function	cAMP
Subcellular Location	Isoform 1: perinuclear region, Isoform 2: ruffle membrane, Isoform 4: Peripheral membrane protein, Isoform 6: perinuclear region, Isoform 7: Membrane, Cytoplasm
Expression	Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lympohocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
Structure	Belongs to cyclic nucleotide phosphodiesterase family. PDE4 subfamily
Alternative Nomenclature	5''-cyclic phosphodiesterase 4A cAMP specific 3 5 cyclic phosphodiesterase 4A DPDE2 dunce like phosphodiesterase E2 PDE 4A PDE4 PDE46 PDE4A11 Phosphodiesterase 4A phosphodiesterase 4A cAMP specific phosphodiesterase E2 dunce homolog phosphodiesterase isozyme 4 phosphodiesterase type 4A11

Protein information supplied by UniProt

FabGennix PDE4A Antibody has been used in 47 Publications

Applications: Confocal microscopy, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, and Western blot

Reactivity: Human, Moose, Mouse, and Rat

Avila, Diana V., et al. "Dysregulation of hepatic cAMP levels via altered Pde4b expression plays a critical role in alcohol‐induced steatosis." The Journal of pathology 240.1 (2016).

PMID: 27287961

Applications: Western Blot

Reactivity: Rat

Massimi, Mara, et al. "Increase of Intracellular Cyclic AMP by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival." Journal of Cellular Biochemistry (2016).

PMID: 27859531

Applications: Western Blot

Reactivity: Human

Kedia, George T., et al. "Expression and Distribution of Phosphodiesterase Isoenzymes in the Human Male Urethra." Urology (2015).

PMID: 25704994

Applications: Immunohistochemistry, Western Blot

Reactivity: Human

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015).

PMID: 25578602

Applications: Immunohistochemistry

Reactivity: Human

Guo, Jiejie, et al. "Etazolate abrogates the lipopolysaccharide (LPS)-induced downregulation of the cAMP/pCREB/BDNF signaling, neuroinflammatory response and depressive-like behavior in mice." Neuroscience 263 (2014).

PMID: 24434771

Applications: Western Blot

Reactivity: Mouse

Zhai, Kui, et al. "Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms." Cellular signalling 26.5 (2014).

PMID: 23347325

Applications: Western Blot

Reactivity: Rat

Amanda, G. Vang, et al. "Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac." Biochemical Journal 456.3 (2013).

PMCID: 24007532

Applications: Western Blot

Reactivity: Mouse

Wang, Zhen-Zhen et al. “RNA Interference-Mediated Phosphodiesterase 4D Splice Variants Knock-down in the Prefrontal Cortex Produces Antidepressant-Like and Cognition-Enhancing Effects.” British Journal of Pharmacology 168.4 (2013).

PMCID: 3631387

Applications: Western Blot

Reactivity: Mouse

Yougbaré, Issaka, et al. "NCS 613 Exhibits Anti-Inflammatory Effects On Pbmcs From Lupus Patients By Inhibiting P38 MAPK And NF-Κb Signalling Pathways While Reducing Proinflammatory Cytokine Production." Canadian Journal Of Physiology & Pharmacology 91.5 (2013).

PMCID: 23656347

Applications: Immunocytochemistry, Western Blot

Reactivity: Human

Schaal, Sandra Marie et al. “The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as a Neuroprotectant Following Spinal Cord Injury.” Ed. Valentin Ceña. PLoS ONE 7.9 (2012).

PMID: 3446989

Applications: Confocal microscopy, Immunofluorescence, Immunohistochemistry, Western Blot

Reactivity: Rat

Click Here for the complete list of all FabGennix PDE4A Antibody publications

Publishing research using PD4A-112AP? Please let us know, we can cite the reference in this datasheet.

To view the PD4A-112AP specification sheet, click the link below:

FabGennix PD4A-112AP Specification Sheet

Customer ratings for PDE4A Antibody

Number of ratings: 1
Average rating: 5

Excellent
from Verified Customer on 12/10/2020

1:500 dilution in Western blot. Excellent results in Sprague Dawley rats liver tissue.

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PDE4A Antibody FITC

PD4A-FITC

PDE4A Antibody BIOTIN

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P-PD4A

PDE4A Positive Control

PC-PD4A

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