Western Blot Positive ControlAdrenocorticotropin Hormone
Verified Applications
WB
Accession #
NCBI: AAX36901
Physical Properties
Quantity
5 Applications
Volume
Inquire
Concentration
The concentration of the sample is empirically set so that 20 µl per lane in Western and blotted with corresponding affinity purified antibody will give a clean band with a high signal to noise ratio on the gel.
Form
Western Blot Positive Control in ready to use SDS sample buffer
Your datasheets states this antibody was raised against "aa 1-70 of ACTH." However, ACTH (AKA corticotropin) is only a 38 aa protein (aa 138-176 of POMC) that is formed from cleavage of POMC, see the uniprot file below. I wanted to confirm if this antibody was raised against amino acids 1-70 or 138-176 of POMC.
The ACTH antibodies were raised against 39 amino acid ACTH hormone. The Specification sheet specifies the rangefrom where the peptide was selected. In this case the 39 AA are the complete ACTH hormone.
ACTH human
1-39
SYS MEH FRW GKP VGK KRR PVK VYP NGA EDE SAE AFP LEF
The peptide was conjugated with a carrier protein for making ACTH antibodies.