MAPK1 Antibody

General Information

Product

MAPK1 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P28482

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis.

Molecular Function

Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm › cytoskeleton › spindle

Nucleus

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Cytoplasm

Structure

Binds both upstream activators and downstream substrates in multimolecular complexes. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4,

Alternative Nomenclature

ERK antibody
ERK-2 antibody
ERT1 antibody
Extracellular signal-regulated kinase 2 antibody
MAP kinase 1 antibody

MAP kinase isoform p42 antibody
MAPK 1 antibody

Mapk1 antibody
MAPK2 antibody
Mitogen-activated protein kinase 1 antibody
P38 antibody
P40 antibody
P41 antibody
p42-MAPK antibody
P42MAPK antibody
PRKM1 antibody
protein kinase, mitogen-activated, 1 antibody