General Information

Product

XFLOP Antibody

Description

Affinity Purified orphan G protein-coupled receptor Xflop Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Xenopus laevis

Immunogen

Synthetic peptide taken within amino acid region 200-250 on Xenopus laevis orphan G protein-coupled receptor Xflop protein.

Accession #

NCBI: NP_001089016.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.50-1.20 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

Q5ID20

Overview

G-protein coupled receptor activity

Molecular Function

Receptor

Subcellular Location

Integral component of membrane

Alternative Nomenclature

XFLOP antibody,
orphan gpcr antibody
gpcr1 antibody
g-protein coupled receptor 1 antibody
orphan G protein-coupled receptor antibody
GPR4 antibody
orphan GPR4 antibody
orphan g protein-coupled receptor 4 XFLOP antibody

General Information

Product

MC4 Receptor Antibody

Description

Affinity Purified Melanocortin receptor 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 10-60 on human MC4R protein.

Accession #

NCBI:  NP_005903.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P32245

Overview

Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP).

Molecular Function

Melanocortin receptor activity, melanocyte-stimulating hormone receptor activity, neuropeptide binding, ubiquitin protein ligase binding

Subcellular Location

cell membrane, multi-pass membrane protein, membrane raft. (Note: Localizes to membrane rafts but is also loated in the cell membrane outside of these regions where channel response to cold is enhanced compared to membrane rafts).

Expression

Brain, placental, and gut tissues.

Alternative Nomenclature

MC4 R antibody

Melanocortin receptor 4 antibody

MGC126851 antibody

MGC138197 antibody

General Information

Product

5HT1B Receptor Antibody

Description

Affinity Purified 5 Hydroxytryptamine 1B receptor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 160-260 on rat 5HT1B

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:50,000

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P28334

Overview

G-protein coupled receptor for serotonin (5-hydroxytryptamine).   Functions as a receptor for various drugs which cause conformational change upon ligand binding that triggers signaling via guanine nucleo-tide binding proteins and modulation of down-stream effectors like adenylate cyclase.  Inhibits adenylate cyclase activity and activates phosphatidylinositol-calcium second messenger system that regulates release of intracellular Calcium ions.

Molecular Function

Drug binding, serotonin receptor activity, serotonin binding

Subcellular Location

Cell membrane; Multi-pass protein

Structure

Homodimer. Heterodimer with HTR1D

Alternative Nomenclature

HTR1DB antibody

S12 antibody

Serotonin 1D beta receptor antibody

General Information

Product

ACK1 Antibody

Description

Affinity Purified Activated CDC42 kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on ACK1 protein.

Accession #

GenBank: L13738.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q07912

Overview

Non-receptor tyrosine-protein and serine/threonine-protein kinase implicated in cell sperading and migration, survival, growth and proliferation.  Transduces extracellular signals to cytosolic and nuclear effectors.

Molecular Function

ATP binding; epidermal growth factor receptor binding; GTPase inhibitor activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine kinase activity

Subcellular Location

Cell membrane; Nucleus; Endosome; Cell junction; adherens junction; Cytoplasmic vessicle membrane; Peripheral membrane protein; Clathrin-coated vessicles; Clathin coated pit

Expression

During progressive stages in breast and prostate cancer

Structure

Forms  complexes with GRB2 and several receptor tyrosine kinases.  Part of collagen stimulated complex.

Alternative Nomenclature

Acetate kinase 1 antibody

Acetokinase 1 antibody

ACK 1 antibody

Activated Cdc42 associated kinase 1 antibody

Activated CDC42 kinase 1 antibody

Activated p21cdc42Hs kinase antibody

p21cdc42Hs antibody

TNK 2 antibody

Tyrosine kinase non-receptor protein 2 antibody

General Information

Product

Adenylate Cyclase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rabbit, Rat

Immunogen

Synthetic peptide corresponding to conserved sequence at or near C terminal end of protein that is unique to rat/mouse Adenylyl Cyclase 1 protein.

Accession #

GenBank: AAC29478 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55- 0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500. Detects a band 127-10 kDa

Protein

Uniprot #

O88444

Overview

This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. Plays a role in the regulation of the circadian rhythm of daytime contrast sensitivity probably by modulating the rhythmic synthesis of cyclic AMP in the retina

Molecular Function

ATP-binding, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Subcellular Location

Membrane; Multi-pass membrane protein. Cytoplasm

Expression

Expressed throughout inner ear development. Brain, retina and adrenal medulla.

Structure

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains

Alternative Nomenclature

3'5' cyclic AMP synthetase antibody

ADCY1 antibody

adenyl cyclase antibody

Adenylate cyclase 1 antibody

ATP pyrophosphate lyase antibody

Brain adenylate cyclase 1 antibody

Ca(2+)/calmodulin activated adenylyl cyclase antibody

EC 4.6.1.1 antibody

General Information

Product

ADCY3 Antibody

Description

Particulate Adenylate cyclase type 3 Antibody Affinity Purified

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Mouse, Rat

Immunogen

Synthetic peptide generated against a sequence unique to PAC3 protein. All peptides were amidated before conjugation.

Accession #

NCBI:  NP_004027

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Polyclonal

Clonality

Rabbit

Determinant

Affinity Purified

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O60266

Overview

Mediates odorant detection via modulation of intracellular cAMP concentration

Molecular Function

Adenylate cyclase activity; cAMP biosynthesis; ATP-binding; Magnesium; Metal binding; Nucleotide-binding

Subcellular Location

Membrane; Multi-pass membrane protein

Expression

Brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle

Structure

Belongs to adenylyl cyclase class-4 and guanylyl cyclase family.  Contains 2 guanylate cyclase domains.

Alternative Nomenclature

ATP pyrophosphate-lyase 3 antibody

olfactive type antibody

General Information

Product

Adenylate Cyclase 4 Antibody

Description

Affinity Purified Adenylate cyclase type 4 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on rat Particulate Adenylyl Cyclase 4 protein.

Accession #

NCBI:  NP_062158

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.526 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100-1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100-1:200

Immunofluorescence

1:100-1:200

Immunohistochemistry

1:100-1:200

Western Blot

1:500

Protein

Uniprot #

Q66HK5

Molecular Function

Nucleotide-binding

Subcellular Location

Membrane

Structure

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family

Alternative Nomenclature

AC4 antibody

Adcy4 antibody

Adenylate cyclase 4 antibody

ATP pyrophosphate-lyase 4 antibody

General Information

Product

ADCY6 Antibody

Description

Affinity Purified Adenylate cyclase type 6 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide corresponding to N-terminal amino acids 13-27 of rat Particulate Adenylyl Cyclase 6 protein. Peptide was covalently modified and conjugated to KLH.

Sequence:

DERKTAWGERNGQKR

Accession #

GenBank: AAD29856.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100-1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100-1:200

Immunofluorescence

1:100-1:200

Immunohistochemistry

1:100-1:200

Western Blot

1:500

Protein

Uniprot #

Q03343

Overview

Membrane bound calcium-inhibitable adenylyl cyclase.

Molecular Function

Adenylate cyclase activity; cAMP biosynthesis; ATP-binding; Magnesium; Metal binding; Nucleotide-binding

Subcellular Location

Membrane; Multi-pass membrane protein; Cell projection; cilium

Structure

Part of a complex containing AKAP5, ADCY5, PDE4C, PKD2. Interacts with RAF1.

Alternative Nomenclature

AC6 

ACVI 

ADCYB 

Adenylate cyclase 6 

ATP pyrophosphate-lyase 6 

Ca(2+) inhibitable adenylyl cyclase 

EC 4.6.1.1 

KIAA0422 

General Information

Product

ADCY7 Antibody

Description

Affinity Purified Particulate Adenylate cyclase type 7 Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on mouse Particulate Adenylyl Cyclase 7 protein.

Accession #

NCBI:  NP_031432.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100-1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100-1:200

Immunofluorescence

1:100-1:200

Immunohistochemistry

1:100-1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P51829

Overview

Membrane bound calcium-inhibitable adenylyl cyclase.

Molecular Function

Adenylate cyclase activity; cAMP biosynthesis; ATP-binding; Magnesium; Metal binding; Nucleotide-binding

Subcellular Location

Membrane; Multi-pass membrane

Expression

Heart, spleen and lung

Structure

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.  Contains 2 guanylate cyclase domains.

Alternative Nomenclature

adenylate cyclase 7 antibody

adenylyl cyclase antibody

ATP pyrophosphate-lyase antibody

KIAA0037 antibody