General Information

Product

Phospho Src Antibody

Description

Affinity Purified Phosphorylated Proto-oncogene tyrosine-protein kinase Src Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope phosphorylated synthetic peptide taken within amino acid region 400-500 on human Src protein.  Phosphorylated Tyr-416 residue.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

Neuronal CSRC tyrosine specific protein kinase antibody

p60 Src antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

Phospho-Src Antibody

Description

Affinity Purified Phosphorylated Proto-oncogene tyrosine-protein kinase Src Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope phosphorylated synthetic peptide taken within amino acid region 200-300 on human Src protein.  Phosphorylated Tyr-215 residue.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

Neuronal CSRC tyrosine specific protein kinase antibody

p60 Src antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

Src Antibody

Description

Proto-oncogene tyrosine-protein kinase Src Antibody C-epitope

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-500 on human Src protein.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.60 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation.

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

c SRC antibody

CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody

EC 2.7.10.2 antibody

Neuronal CSRC tyrosine specific protein kinase antibody

Neuronal SRC antibody

Oncogene SRC antibody

p60 Src antibody

Proto-oncogene tyrosine-protein kinase Src antibody

SRC proto oncogene non receptor tyrosine kinase antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

Tyrosine protein kinase SRC1 antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

Src Antibody

Description

Affinity Purified Proto-oncogene tyrosine-protein kinase Src Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-300 on human Src protein.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.60 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

c SRC antibody

CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody

EC 2.7.10.2 antibody

Neuronal CSRC tyrosine specific protein kinase antibody

Neuronal SRC antibody

Oncogene SRC antibody

p60 Src antibody

Proto oncogene tyrosine protein kinase Src antibody

SRC Oncogene antibody

SRC proto oncogene non receptor tyrosine kinase antibody

SRC1 antibody

vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

TIE2 Antibody

Description

Affinity Purified Tyrosine-protein kinase receptor Tie-1 isoform 2 Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 540-590 on human Tyrosine-protein kinase receptor Tie-1 isoform 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02763

Overview

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Integral component of membrane

Expression

Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma. Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family.  Contains protein kinase domain.

Alternative Nomenclature

Angiopoietin 1 receptor antibody

CD202b antibody

Endothelial tyrosine kinase antibody

Endothelium specific receptor tyrosine kinase 2 antibody

Hyk antibody

p140 TEK antibody

TEK antibody

tek tyrosine kinase antibody

TIE 2 antibody

Tunica interna endothelial cell kinase antibody

Tyrosine kinase with Ig and EGF homology domains 2 antibody

Tyrosine protein kinase receptor TEK antibody

Tyrosine protein kinase receptor TIE 2 antibody

Venous malformations multiple cutaneous and mucosal antibody

VMCM 1 antibody

VMCM antibody

General Information

Product

TrkA Antibody

Description

Affinity Purified Tyrosine Receptor Kinase A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 360-410 on human TrkA protein.

Accession #

GenBank: BAA34355.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04629

Overview

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.  Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.

Molecular Function

Developmental Protein; Kinase; Receptor; Transferase; Tyrosine-protein kinase

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early & Late endosome membrane

Expression

Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 protein kinase domain.

Alternative Nomenclature

CIPA antibody

gp140trk antibody

High affinity nerve growth factor receptor antibody

MTC antibody

Neurotrophic tyrosine kinase receptor type 1 antibody

Ntrk1 antibody

Oncogene TRK antibody

p140-TrkA antibody

Slow nerve growth antibody

TRK antibody

Trk-A antibody

TRK1 antibody

TRK1-transforming tyrosine kinase protein antibody

Tropomyosin-related kinase A antibody

Tyrosine kinase receptor A antibody

General Information

Product

VEGFR1 Antibody

Description

Affinity Purified Vascular Endothelial Growth Factor Receptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1250-1300 on human Vascular endothelial growth factor receptor 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P17948

Overview

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

Molecular Function

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1 :Cell membrane; Single-pass type I membrane protein

Endosome Note: Autophosphorylation promotes ubiquitination and endocytosis.

Isoform 2 :Secreted 

Isoform 3 :Secreted

Isoform 4 :Secreted

Isoform 5 :Cytoplasm

Isoform 6 :Cytoplasm 

Isoform 7 :Cytoplasm 

Expression

Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).

Structure

The second and third Ig-like C2-type (immunoglobulin-like) domains are sufficient for VEGFA binding. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 7 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.

Alternative Nomenclature

EC 2.7.10.1 

FLT 

FLT1 

Fms like tyrosine kinase 1 

FRT 

Soluble VEGF receptor 1 14 

Soluble VEGFR1 variant 2 

Soluble VEGFR1 variant 21 

Tyrosine protein kinase FRT 

Tyrosine protein kinase receptor FLT 

Vascular endothelial growth factor vascular permeability factor receptor 

Vascular permeability factor receptor 1 

VEGFR1

General Information

Product

GTPase HRAS Antibody

Description

Affinity Purified Transforming p21 Ras Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human GTPase HRAS protein.

Accession #

NCBI:  NP_005334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01112 

Overview

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Molecular Function

GTP binding, Protein C-terminus binding

Subcellular Location

Cell membrane; Lipid anchor; Cytoplasmic side; Gogli appartus membrane; Lipid-anchor

Structure

In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3. Interacts with HSPD1. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain).

Alternative Nomenclature

c bas/has antibody

c has/bas p21 protein antibody

c ras Ki 2 protein antibody

c-H-ras antibody

CTLO antibody

G1III6 N ras antibody

GTP and GDP binding peptide B antibody

GTPase HRas antibody

H Ras 1 antibody

H ras oncogene antibody

H RasIDX antibody

Ha Ras1 proto oncoprotein antibody

HAMSV antibody

Harvey rat sarcoma viral oncoprotein antibody

HRAS1 antibody

K ras antibody

N ras antibody

p19 H RasIDX protein antibody

p21ras antibody

Ras family small GTP binding protein H Ras antibody

RASH1 antibody

Transformation gene oncogene HAMSV antibody

Transforming protein p21 antibody

v Ha ras Harvey rat sarcoma viral oncogene homolog antibody

VH Ras antibody

General Information

Product

GTPase HRAS Antibody

Description

Affinity Purified Transforming p21 Ras Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 139-189 on human GTPase HRAS protein.

Accession #

NCBI:  NP_005334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01112 

Overview

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Molecular Function

GTP binding, Protein C-terminus binding

Subcellular Location

Cell membrane; Lipid anchor; Cytoplasmic side; Gogli appartus membrane; Lipid-anchor

Structure

In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3. Interacts with HSPD1. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain).

Alternative Nomenclature

c bas/has antibody

c has/bas p21 protein antibody

c ras Ki 2 protein antibody

c-H-ras antibody

CTLO antibody

G1III6 N ras antibody

GTP and GDP binding peptide B antibody

GTPase HRas antibody

H Ras 1 antibody

H ras oncogene antibody

H RasIDX antibody

Ha Ras1 proto oncoprotein antibody

HAMSV antibody

Harvey rat sarcoma viral oncoprotein antibody

HRAS1 antibody

K ras antibody

N ras antibody

p19 H RasIDX protein antibody

p21ras antibody

Ras family small GTP binding protein H Ras antibody

RASH1 antibody

Transformation gene oncogene HAMSV antibody

Transforming protein p21 antibody

v Ha ras Harvey rat sarcoma viral oncogene homolog antibody

VH Ras antibody

General Information

Product

GTPase HRAS Antibody

Description

Affinity Purified Transforming p21 Ras Antibody Mid-region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human GTPase HRAS protein.

Accession #

NCBI:  NP_005334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01112 

Overview

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Molecular Function

GTP binding, Protein C-terminus binding

Subcellular Location

Cell membrane; Lipid anchor; Cytoplasmic side; Gogli appartus membrane; Lipid-anchor

Structure

In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3. Interacts with HSPD1. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain).

Alternative Nomenclature

c bas/has antibody

c has/bas p21 protein antibody

c ras Ki 2 protein antibody

c-H-ras antibody

CTLO antibody

G1III6 N ras antibody

GTP and GDP binding peptide B antibody

GTPase HRas antibody

H Ras 1 antibody

H ras oncogene antibody

H RasIDX antibody

Ha Ras1 proto oncoprotein antibody

HAMSV antibody

Harvey rat sarcoma viral oncoprotein antibody

HRAS1 antibody

K ras antibody

N ras antibody

p19 H RasIDX protein antibody

p21ras antibody

Ras family small GTP binding protein H Ras antibody

RASH1 antibody

Transformation gene oncogene HAMSV antibody

Transforming protein p21 antibody

v Ha ras Harvey rat sarcoma viral oncogene homolog antibody

VH Ras antibody