General Information
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Product
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TRF2 Antibody BIOTIN-Conjugated
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Description
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BIOTIN-Conjugated Telomeric Repeat-binding Factor 2 Antibody Mid-Region
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Verified Applications
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ELISA, IMM, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 200-250 on human Telomeric repeat-binding factor 2 protein.
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Accession #
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GenBank: AAB81135.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.50 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Determinant
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Mid-Region
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:20,000
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ELISA
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1:20,000
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Immunoprecipitation
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1:200
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Western Blot
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1:500-1:750
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Protein
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Uniprot #
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Q15554
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Overview
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Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
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Molecular Function
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Chromatin binding; Double-stranded telomeric DNA binding; Protein C-terminus binding; Protein homodimerization activity; Telomeric DNA binding
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Subcellular Location
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Nucleus; Chromosome > telomere
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Expression
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Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
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Structure
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Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct.
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Alternative Nomenclature
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Telomeric DNA binding protein antibody
Telomeric repeat binding factor 2 antibody
TERF 2 antibody
TRBF 2 antibody
TRF 2 antibody
TTAGGG repeat-binding factor 2 antibody
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