General Information

Product

TIA1 Antibody

Description

Affinity Purified Nucleolysin TIA-1 isoform p40 Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 414-464 on human TIA1 protein.

Accession #

GenBank: BAD92448.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50

Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:500

Protein

Uniprot #

P31483

Overview

Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Molecular Function

AU-rich element binding, nucleotide binding, poly(A) binding, poly (A) RNA binding

Subcellular Location

Cytoplasmic granule, nucleus. (Note: accumulates in cytoplasmic stress granules following cellular damage

Alternative Nomenclature

Cytotoxic granule associated RNA binding protein 1 antibody

Nucleolysin TIA 1 isoform p40 antibody

p40 TIA 1 antibody

p40-TIA-1 (containing p15-TIA-1) antibody

RNA binding protein TIA 1 antibody

T-cell-restricted intracellular antigen-1 antibody

TIA 1 cytotoxic granule associated RNA binding protein antibody

TIA1 cytotoxic granule associated RNA binding protein like 1 antibody

TIA1 protein antibody

TIAL1 antibody

TIAR antibody

General Information

Product

TIA1 Antibody Affinity Purified

Description

Affinity Purified Nucleolysin TIA-1 isoform p40 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human TIA1 protein.

Accession #

GenBank: BAD92448.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50

Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:500

Protein

Uniprot #

P31483

Overview

Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Molecular Function

AU-rich element binding, nucleotide binding, poly(A) binding, poly (A) RNA binding

Subcellular Location

Cytoplasmic granule, nucleus. (Note: accumulates in cytoplasmic stress granules following cellular damage

Alternative Nomenclature

Cytotoxic granule associated RNA binding protein 1 antibody

Nucleolysin TIA 1 isoform p40 antibody

p40 TIA 1 antibody

p40-TIA-1 (containing p15-TIA-1) antibody

RNA binding protein TIA 1 antibody

T-cell-restricted intracellular antigen-1 antibody

TIA 1 cytotoxic granule associated RNA binding protein antibody

TIA1 cytotoxic granule associated RNA binding protein like 1 antibody

TIA1 protein antibody

TIAL1 antibody

TIAR antibody

General Information

Product

Mitofusin 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 543-593 on human Mitofusin 2 protein.

Accession #

NCBI:  NP_055689

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunohistochemistry

1:100

Western Blot

1:500

Protein

Uniprot #

O95140

Overview

Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.

Molecular Function

Hydrolase; GTP-binding, Nucleotide binding

Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein

Expression

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

Structure

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA famil. Mitofusin subfamily. Contains 1 dynamin-type G (guanine nucleotide-binding) domain.

Alternative Nomenclature

CMT2A antibody
CMT2A2 antibody
CPRP 1 antibody
EC 3.6.5.- antibody
Fzo antibody
HSG antibody
hyperplasia suppressor gene antibody
Hypertension related protein 1 antibody
KIAA0214 antibody
MARF antibody
MFN 2 antibody

Mitochondrial assembly regulatory factor antibody
Mitofusin-2 antibody

Transmembrane GTPase MFN2 antibody

General Information

Product

IRE1 Antibody 

Description

Affinity Purified Serine/Threonine-protein kinase/endoribonuclease IRE1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-977 on human IRE1A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O75460

Overview

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Molecular Function

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein

Expression

Ubiquitously expressed. High levels observed in pancreatic tissue.

Structure

Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1 (By similarity). Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic intera

Alternative Nomenclature

Endoplasmic reticulum (ER) to nucleus signalling 1 antibody Endoribonuclease antibody
ER to nucleus signaling 1 antibody

ERN 1 antibody

hIRE 1p antibody
Inositol requiring 1 antibody
Inositol requiring protein 1 antibody
IRE 1 antibody
IRE 1a antibody
IRE 1P antibody
Ire1 alpha antibody

IRE1P antibody
MGC163277 antibody
MGC163279 antibody
Protein kinase/endoribonuclease antibody
RGD1559716 antibody
Serine/threonine protein kinase/endoribonuclease IRE1 antibody

General Information

Product

Humanin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide corresponding to amino acid region 5-24 on Humanin protein.

Sequence:

GFSCLLLSTTATDLPVKRRT

Accession #

NCBI:  NP_001177399.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IVG9

Overview

Plays a role as a neuroprotective factor. Protects against death induced by multiple different familial Alzheimer disease genes and beta amyloid proteins in Alzheimer disease. Suppresses apoptosis by binding to BAX and preventing the translocation of BAX from the cytosol to mitochondria. Binds to IGFBP3 and specifically blocks IGFBP3-induced cell death Induces chemotaxis of mononuclear phagocytes via FPR2. Reduces the aggregation and fibrillary formation by suppressing the effect of APP on mononuclear phagocytes and acts by competitively inhibiting the access of FPRL1 to APP.

Molecular Function

Receptor antagonist activity; Apoptosis; Leukocyte chemotaxis

Subcellular Location

Secreted; Cytoplasm

Expression

Expressed in the heart, skeletal muscles, kidney and liver. Lesser but significant expression is observed in the brain and the gastrointestinal tract. Expressed in the AD brain, where it is found in some of the large intact neurons of the occipital lobes and small and round reactive glial cells in the hippocampus.

Structure

Largely unstructured in aqueous solution.

Alternative Nomenclature

HN antibody

HUNIN Antibody

MT-RNR2 antibody

General Information

Product

GTPase HRAS Antibody

Description

Affinity Purified Transforming p21 Ras Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human GTPase HRAS protein.

Accession #

NCBI:  NP_005334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01112 

Overview

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Molecular Function

GTP binding, Protein C-terminus binding

Subcellular Location

Cell membrane; Lipid anchor; Cytoplasmic side; Gogli appartus membrane; Lipid-anchor

Structure

In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3. Interacts with HSPD1. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain).

Alternative Nomenclature

c bas/has antibody

c has/bas p21 protein antibody

c ras Ki 2 protein antibody

c-H-ras antibody

CTLO antibody

G1III6 N ras antibody

GTP and GDP binding peptide B antibody

GTPase HRas antibody

H Ras 1 antibody

H ras oncogene antibody

H RasIDX antibody

Ha Ras1 proto oncoprotein antibody

HAMSV antibody

Harvey rat sarcoma viral oncoprotein antibody

HRAS1 antibody

K ras antibody

N ras antibody

p19 H RasIDX protein antibody

p21ras antibody

Ras family small GTP binding protein H Ras antibody

RASH1 antibody

Transformation gene oncogene HAMSV antibody

Transforming protein p21 antibody

v Ha ras Harvey rat sarcoma viral oncogene homolog antibody

VH Ras antibody

General Information

Product

GTPase HRAS Antibody

Description

Affinity Purified Transforming p21 Ras Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 139-189 on human GTPase HRAS protein.

Accession #

NCBI:  NP_005334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01112 

Overview

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Molecular Function

GTP binding, Protein C-terminus binding

Subcellular Location

Cell membrane; Lipid anchor; Cytoplasmic side; Gogli appartus membrane; Lipid-anchor

Structure

In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3. Interacts with HSPD1. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain).

Alternative Nomenclature

c bas/has antibody

c has/bas p21 protein antibody

c ras Ki 2 protein antibody

c-H-ras antibody

CTLO antibody

G1III6 N ras antibody

GTP and GDP binding peptide B antibody

GTPase HRas antibody

H Ras 1 antibody

H ras oncogene antibody

H RasIDX antibody

Ha Ras1 proto oncoprotein antibody

HAMSV antibody

Harvey rat sarcoma viral oncoprotein antibody

HRAS1 antibody

K ras antibody

N ras antibody

p19 H RasIDX protein antibody

p21ras antibody

Ras family small GTP binding protein H Ras antibody

RASH1 antibody

Transformation gene oncogene HAMSV antibody

Transforming protein p21 antibody

v Ha ras Harvey rat sarcoma viral oncogene homolog antibody

VH Ras antibody

General Information

Product

GTPase HRAS Antibody

Description

Affinity Purified Transforming p21 Ras Antibody Mid-region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human GTPase HRAS protein.

Accession #

NCBI:  NP_005334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01112 

Overview

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Molecular Function

GTP binding, Protein C-terminus binding

Subcellular Location

Cell membrane; Lipid anchor; Cytoplasmic side; Gogli appartus membrane; Lipid-anchor

Structure

In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3. Interacts with HSPD1. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain).

Alternative Nomenclature

c bas/has antibody

c has/bas p21 protein antibody

c ras Ki 2 protein antibody

c-H-ras antibody

CTLO antibody

G1III6 N ras antibody

GTP and GDP binding peptide B antibody

GTPase HRas antibody

H Ras 1 antibody

H ras oncogene antibody

H RasIDX antibody

Ha Ras1 proto oncoprotein antibody

HAMSV antibody

Harvey rat sarcoma viral oncoprotein antibody

HRAS1 antibody

K ras antibody

N ras antibody

p19 H RasIDX protein antibody

p21ras antibody

Ras family small GTP binding protein H Ras antibody

RASH1 antibody

Transformation gene oncogene HAMSV antibody

Transforming protein p21 antibody

v Ha ras Harvey rat sarcoma viral oncogene homolog antibody

VH Ras antibody

General Information

Product

Human Lactoferrin Antibody

Verified Applications

ELISA, ICC/IF, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human

Immunogen

Full length native protein purified human Lactoferrin.

Accession #

GeneBank: AAA59511

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.8 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P02788

Overview

Iron binding transport proteins which bind two Fe3+ ions in association with the binding of an anion usually bicarbonate. Binds to bacterial surface and is crucial for the bactericidal functions.  Has some antiviral activity against papillomavirus infection.

Molecular Function

DNA binding, Heparin Binding, Iron, metal Binding, Immunity

Subcellular Location

Secreted; Cytoplasmic granule; Nucleus

Expression

Saliva, tears, serum, plasma, urine, distal collecting tubules in the medulla, peripheral blood leukocytes.

Structure

Belongs to transferrin family; Contains 2 transferrin-like domains

Alternative Nomenclature

CKRX antibody

GIG12 antibody

Growth inhibiting protein 12 antibody

HLF2 antibody

Homo sapiens chemokine receptor X antibody

Kaliocin 1 antibody

Lactoferrin antibody

Lactoferroxin A antibody

Lactoferroxin B antibody

Lactoferroxin-C antibody

Lactotransferrin antibody

LF antibody

LTF antibody

Neutrophil lactoferrin antibody

Talalactoferrin alfa antibody

General Information

Product

Bcl-XL Antibody

Description

Affinity Purified Bcl-2-like protein 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human BCL-2-like protein 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q07817

Overview

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F1F0 activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Isoform Bcl-X(S) promotes apoptosis.

Molecular Function

BH3 domain binding; Identical protein binding; Protein heterodimerization and homodimerization activity; protein kinase binding

Subcellular Location

Mitochondrion inner membrane

Mitochondrion outer membrane

Mitochondrion matrix

Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane

Cytoplasm › cytosol

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Nucleus membrane ; Single-pass membrane protein ; Cytoplasmic side

Note: After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner. Localizes to the centrosome when phosphorylated at Ser-49.

Expression

Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Structure

The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.

Alternative Nomenclature

Apoptosis regulator Bcl X antibody

B cell lymphoma 2 like antibody

B2CL1_HUMAN antibody

Bcl 2 like 1 protein antibody

Bcl X antibody

Bcl xL antibody

BCL XL/S antibody

Bcl xS antibody

Bcl-2-like protein 1 antibody

Bcl2 Like 1 antibody

Bcl2 related gene antibody

BCL2L antibody

BCLX antibody

PPP1R52 antibody

Protein phosphatase 1 regulatory subunit 52 antibody