General Information

Product

STING / TMEM173 Antibody

Description

Affinity Purified Stimulator of interferon genes protein isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acid region 332-342. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to a carrier protein using hetero bifunctional cross linker.

Sequence:

SFSLLSSQEVLRHLLRQEEKEEVT

Accession #

NCBI:  NP_938023

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

86WV6

Overview

Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm.

Molecular Function

cyclic-di-GMP binding, cyclic-GMP-AMP binding, identical protein binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasm › perinuclear region. Cytoplasm

Expression

Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages.

Structure

Associates with the MHC-II complex (By similarity). Homodimer; 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization. Interacts with DDX58/RIG-I, MAVS and SSR2. Interacts with RNF5 and TRIM56. Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta. Interacts with IFIT1 and IFIT2.

Alternative Nomenclature

endoplasmic reticulum IFN stimulator antibody

ERIS antibody

FLJ38577 antibody

Mediator of IRF3 activation antibody

MITA antibody

Mitochondrial mediator of IRF3 activation antibody

MPYS antibody

N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody

NET23 antibody

Stimulator of interferon genes antibody

STING antibody

Tmem173 antibody

Transmembrane protein 173 antibody

General Information

Product

CXCR1 Antibody Affinity Purified

Description

Affinity Purified Chemokine (C-X-C motif) receptor 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Goat

Species Cross Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on mouse CXCR1 protein.

Accession #

NCBI:  NM_178241.4

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.50-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q810W6

Overview

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.

Molecular Function

Chemotaxis

Subcellular Location

Cell membrane; Multi-pass membrane protein

Structure

Belongs to the G-protein coupled receptor 1 family

Alternative Nomenclature

C C antibody

C C CKR 1 antibody

C-X-C chemokine receptor type 1 antibody

CCCKR1 antibody

CD 181 antibody

CD128 antibody

CKR 1 antibody

CMKAR1 antibody

CXC-R1 antibody

High affinity interleukin-8 receptor A antibody

IL 8 receptor type 1 antibody

IL 8R A antibody

General Information

Product

KIF13A Antibody

Description

Affinity Purified Kinesin-like protein KIF13A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1700-1800 on human KIF13A isoform a protein.

Accession #

NCBI:  NP_071396.4

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9H1H9

Overview

Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis.

Molecular Function

Motor protein

Subcellular Location

Cytoplasm, Cytoskeleton, Endosome, Golgi apparatus, Membrane, Microtubule

Expression

highest levels in heart, brain and skeletal muscle

Structure

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

Alternative Nomenclature

bA500C11.2 antibody

FLJ27232 antibody

Homolog of mouse KIF13A mannose 6 phosphate receptor transporter antibody

KIF 13A antibody

Kinesin family member 13A antibody

Kinesin like protein KIF13A antibody

Kinesin like protein RBKIN antibody

RBKIN antibody

General Information

Product

IL12 p35 Antibody Affinity Purified

Description

Affinity Purified Interleukin 12 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 210-260 on human IL12P35 subunit protein.

Accession #

GenBank: AAD16432.1 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O60595

Molecular Function

interleukin-12 beta subunit binding, protein heterodimerization activity

Alternative Nomenclature

CLMF antibody

CLMF p35 antibody

Cytotoxic lymphocyte maturation factor 1 p35 antibody

IL-12 subunit p35 antibody

IL-12A antibody

Interleukin 12 alpha chain precursor antibody

Interleukin 12 p35 antibody

Natural killer cell stimulatory factor 1 35 kD subunit antibody

NKSF1 antibody

General Information

Product

SOCS3 Antibody

Description

Affinity Purified Suppresor of Cytokine Signaling 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 175-225 on human SOCS3 protein.

Accession #

GenBank:  CAG46495

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q6FI39

Molecular Function

Protein modification; protein ubiquitination.

Subcellular Location

Cytoplasm

Expression

Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Alternative Nomenclature

ATOD4 antibody

CIS 3 antibody

Cish3 antibody

Cytokine induced SH2 protein 3 antibody

E2a Pbx1 target gene in fibroblasts 10 antibody

EF 10 antibody

SOCS 3 antibody

SSI3 antibody

STAT induced STAT inhibitor 3 antibody

Suppressor of cytokine signaling 3 antibody

General Information

Product

TICAM2 Antibody

Description

Affinity Purified TRIF-related adapter molecule Antibody

Verified Applications

DB, ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on TRIF-related adapter molecule.

Accession #

GenBank: AAO74498.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q86XR7

Overview

Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4. May also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB; however, reports are controversial. Involved in IL-18 signaling and is proposed to function as a sorting adaptor for MYD88 in IL-18 signaling during adaptive immune response.

Molecular Function

phospholipid binding, signal transducer activity

Subcellular Location

Isoform 1 : Cytoplasm. Golgi apparatus. Cell membrane. Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum.

Expression

Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).

Structure

Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs i

Alternative Nomenclature

Putative NF-kappa-B-activating protein 502 antibody

TICAM2 antibody

TIR containing adapter molecule 2 antibody

TIR domain-containing adapter molecule 2 antibody

TIRAP3 antibody

TIRP antibody

Toll-like receptor adaptor protein 3 antibody

Toll/interleukin-1 receptor domain-containing protein antibody

TRAM antibody

TRIF-related adapter molecule antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 130-180 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 and 950-976 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit Antibody Mid-Region

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 430-480 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody