General Information

Product

EDG8 Antibody

Description

Affinity Purified Sphingosine 1-phosphate receptor 5 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 380-400 of rat S1P5R/EDG8 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9JKM5

Overview

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G(12) subclass of heteromeric G-proteins.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of

Alternative Nomenclature

EDG 8 antibody

Endothelial differentiation G protein coupled receptor 8 antibody

Endothelial differentiation sphingolipid G protein coupled receptor 8 antibody

S1P receptor 5 antibody

S1P receptor Edg 8 antibody

Sphingosine 1 Phosphate Receptor 5 antibody

Sphingosine 1-phosphate receptor Edg-8 antibody

SPPR2 antibody

General Information

Product

SPHK1 Antibody

Description

Affinity Purified Sphingosine kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human SPHK1 protein.

Accession #

GenBank: CAM17117.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8CI15

Overview

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Molecular Function

ATP binding, calmodulin binding, DNA binding, magnesium ion binding, NAD+kinase activity, sphinganine kinase activity

Subcellular Location

Cytoplasm, nucleus, cell membrane

Alternative Nomenclature

SK1 antibody

Sphingosine Kinase 1 antibody

SPHK 1 antibody

SPHK antibody

SPK antibody

SPK 1 antibody

General Information

Product

SREBP1 Antibody Affinity Purified

Description

Affinity Purified Sterol regulatory element-binding protein 1 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 950-1000 on human Sterol regulatory element binding transcription factor 1 protein.

Accession #

GenBank: AAH63281.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P36956

Overview

Transcriptional activator required for lipid homeostasis.

Molecular Function

Activator

Subcellular Location

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

Expression

liver, adrenal gland, lung, ovary, hepatoma cell lines, kidney, brain, white fat, and muscle.

Structure

Belongs to the SREBP family

Alternative Nomenclature

ADD 1 antibody

Class D basic helix-loop-helix protein 1 antibody

Processed sterol regulatory element-binding protein 1 antibody

SREBF 1 antibody

SREBP1 antibody

Sterol regulatory element-binding transcription factor 1 antibody

General Information

Product

SREBP2 Antibody

Description

Affinity Purified Sterol regulatory element binding protein-2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 640-690 on human Sterol regulatory element binding protein-2.

Accession #

GenBank: AAA50746.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q12772

Overview

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

Molecular Function

Activator, E-box binding, protein C-terminus binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein, Golgi apparatus membrane; Multi-pass membrane protein, Cytoplasmic vesicle > COPII-coated vesicle membrane; Multi-pass membrane protein

Expression

Ubiquitously expressed in adult and fetal tissues.

Structure

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF13.

Alternative Nomenclature

AI608257 antibody

bHLHd2 antibody

Class D basic helix-loop-helix protein 2 antibody

Processed sterol regulatory element-binding protein 2 antibody

SREBF 2 antibody

SREBP2 antibody

Sterol regulatory element-binding transcription factor 2 antibody

General Information

Product

PBR Antibody

Description

Affinity Purified Mitochondrial benzodiazepine receptor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on 18 kDa mitochondrial benzodiazepine receptor protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.60-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P30536

Overview

Can bind protoporphyrin IX and may play a role in transport of porphyrins and heme. Promotes transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism.

Molecular Function

Benzodiazepine receptor activity, cholesterol binding

Subcellular Location

Mitochondrian membrane, Multi-pass membrane protein

Expression

Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids

Alternative Nomenclature

Benzodiazapine receptor (peripheral) antibody

Benzodiazepine peripheral binding site antibody

BPBS antibody

BZRP antibody

DBI antibody

IBP antibody

Isoquinoline carboxamide-binding protein antibody

MBR antibody

mDRC antibody

Mitochondrial benzodiazepine receptor antibody

PBR antibody

PBS antibody

Peripheral benzodiazepine receptor antibody

Peripheral benzodiazepine receptor-related protein antibody

pk18 antibody

PKBS antibody

PTBR antibody

Ptbzr antibody

PTBZR02 antibody

RATPTBZR02 antibody

translocator protein (18kDa) antibody

Translocator protein antibody

TSPO antibody

Tspo1 antibody

General Information

Product

Annexin A6 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-560 on human Annexin A6 protein.

Accession #

GenBank: AAH17046

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P08133

Overview

Regulates release of calcium ions from intracellular stores.  Associated with CD21

Molecular Function

Calcium and phospholipid-binding

Subcellular Location

Cytoplasm; Melanosome

Expression

Induced by Epstein-Barr virus

Structure

Pair of annexin repeats may form one binding site for calcium and phospholipid.  Contains 8 annexin repeats.

Alternative Nomenclature

67 kDa calelectrin antibody

ANX 6 antibody

Calcium binding protein p68 antibody

Calphobindin II antibody

CBP68 antibody

Chromobindin 20 antibody

p68 antibody

P70 antibody

Protein III antibody

General Information

Product

Annexin A9 Antibody

Description

Affinity Purified annexin A9 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human Annexin A9 protein.

Accession #

NCBI:  NP_003559

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O76027

Overview

Low affinity receptor for acetylcholine known to be targeted by disease-causing pemphigus vulgaris antibodies in keratinocytes.

Molecular Function

acetylcholine receptor activity; phospholipid binding; synaptic transmission; calcium ion binding

Subcellular Location

Cell surface; cytosol; extracellular vesicular exosome

Expression

Expressed in the stratified squamous skin epithelium, but not in epithelia of other types.

Structure

Homodimer

Alternative Nomenclature

annexin 31 antibody

ANX31 antibody

ANXA9 antibody

Pemphaxin antibody

General Information

Product

PDE8A Antibody

Description

Affinity Purified Pan Phosphodiesterase 8A Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic cyclic peptide from near the N terminus common to all PDE8A variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500-1:2,000

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.

Molecular Function

3',5'-cyclic-AMP phosphodiesterase activity, Hydrolase, Metal-binding

Subcellular Location

Cytosol

Expression

Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.

Structure

Belongs to cyclic nucleotide phosphodiesterase family. PDE8B subfamily.  Contains 1 PAC (Pas-associated C-terminal) domain.  Contains 1 PAS (PER-ARNT-SIM) domain.

Alternative Nomenclature

5''-cyclic phosphodiesterase 8A antibody

cAMP specific cyclic nucleotide phosphodiesterase 8A antibody

FLJ16150 antibody

High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody

HsT19550 antibody

PDE 8A antibody

Phosphodiesterase 8A antibody

Weakly similar to 3'5'-cyclic-nucleotide phosphodiesterase antibody

General Information

Product

PDE8A Antibody

Description

Affinity Purified Pan Phosphodiesterase 8A Antibody Mid-Region

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic cyclic peptide from mid-region common to all PDE8A variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500-1:2,000

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.

Molecular Function

3',5'-cyclic-AMP phosphodiesterase activity, Hydrolase, Metal-binding

Subcellular Location

Cytosol

Expression

Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.

Structure

Belongs to cyclic nucleotide phosphodiesterase family. PDE8B subfamily.  Contains 1 PAC (Pas-associated C-terminal) domain.  Contains 1 PAS (PER-ARNT-SIM) domain.

Alternative Nomenclature

5''-cyclic phosphodiesterase 8A antibody

cAMP specific cyclic nucleotide phosphodiesterase 8A antibody

FLJ16150 antibody

High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody

HsT19550 antibody

PDE 8A antibody

Phosphodiesterase 8A antibody

Weakly similar to 3'5'-cyclic-nucleotide phosphodiesterase antibody

General Information

Product

PDE8A Antibody

Description

Affinity Purified Pan Phosphodiesterase 8A Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic cyclic peptide from C terminus common to all PDE8A variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.

Molecular Function

3',5'-cyclic-AMP phosphodiesterase activity, Hydrolase, Metal-binding

Subcellular Location

Cytosol

Expression

Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.

Structure

Belongs to cyclic nucleotide phosphodiesterase family. PDE8B subfamily.  Contains 1 PAC (Pas-associated C-terminal) domain.  Contains 1 PAS (PER-ARNT-SIM) domain.

Alternative Nomenclature

5''-cyclic phosphodiesterase 8A antibody

cAMP specific cyclic nucleotide phosphodiesterase 8A antibody

FLJ16150 antibody

High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody

HsT19550 antibody

PDE 8A antibody

Phosphodiesterase8A antibody

Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody