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SGLT1 Antibody

Product no.: SGLT-112AP

$285.00

General Information
Product	SGLT1 Antibody
Description	Affinity Purified Sodum/glucose cotransporter 1 Antibody C-epitope
Verified Applications	CM, Dot Blot, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat, Sheep
Immunogen	Synthetic peptide corresponding to amino acids 603-623 of Human SGLT1.
Accession #	NCBI: NP_000334.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	C-epitope
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:4,000
ELISA	1:4,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:200
Western Blot	1:500-1:1,000
Protein
Uniprot #	P13866
Overview	Actively transports glucose into cells by Na+ cotransport with a Na+ to glucose coupling ratio of 2:1.
Molecular Function	glucose:sodium symporter activity
Subcellular Location	Membrane
Expression	Expessed mainly in intestine and kidney.
Structure	Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Alternative Nomenclature	D22S675 antibody High affinity sodium glucose cotransporter 1 antibody Human Na+/glucose cotransporter 1 antibody Na(+)/glucose cotransporter 1 antibody NAGT antibody SGLT1 antibody SLC5A1 antibody Sodium glucose cotransporter 1 antibody Solute carrier family 5 member 1 antibody

Protein information supplied by UniProt

To view the Specification Sheet for SGLT1 Antibody, please click the link below:

SGLT1 Antibody | FabGennix

A customer wants to know that this product is specific to SGLT1 (That is not reacted with SGLT2). Is this product tested in being specific to SGLT1? If you have tested data, please send me. Or if not data, could you tell me something proving to be specific to SGLT1?
We would like to know whether SGLT1 Positive Control (PC-SGLT) are developed from insect sources or baculovirus expression systems.
I'm currently working on 5 different breast cancer cell lines and I'm using yours standard protocol for WB in that particular Antibodies, with 40ug of protein instead 60 (I have less nonspecific bands). My WB shows the band at approx. 58kDa in each cell line. I've checked UniProt database for SGLT1 and indeed there are 2 isoforms of this protein. The difference is about 120aas on the beginning of the protein sequence. I'm just curious why my antibodies recognizes only one isoform (the shorter protein) instead of two though the immunogen covers the opposite end of SGLT1 sequence (C-terminus) presents in both isoforms. For now, I'm pretty confused what protein I see in my WB.

A customer wants to know that this product is specific to SGLT1 (That is not reacted with SGLT2). Is this product tested in being specific to SGLT1? If you have tested data, please send me. Or if not data, could you tell me something proving to be specific to SGLT1?
1. Our SGLT1 antibodies, SGLT-101AP and SGLT-112AP, are epitope specific antibodies. They are raised against synthetic peptides that are unique to the SGLT1 protein and are not present in SGLT2. These antibodies do not react to random peptide in ELISA and in Dot Blot assays. These antibodies are affinity purified on immobilized antigen based affinity chromatography. Based on these observations, they will not cross react with SGLT2 proteins.
We would like to know whether SGLT1 Positive Control (PC-SGLT) are developed from insect sources or baculovirus expression systems.
1. SGLT1 Positive Control (PC-SGLT1.m and PC-SGLT.C) are not obtained from insect sells, SF9 cells or other cells. It is a partially purified protein obtained from normal enterocytes. The preparation is in SDS-Sample buffer and does not contain any preservatives. The shelf life is 1 year at -20⁰C and 6 months at 4⁰C.
I'm currently working on 5 different breast cancer cell lines and I'm using yours standard protocol for WB in that particular Antibodies, with 40ug of protein instead 60 (I have less nonspecific bands). My WB shows the band at approx. 58kDa in each cell line. I've checked UniProt database for SGLT1 and indeed there are 2 isoforms of this protein. The difference is about 120aas on the beginning of the protein sequence. I'm just curious why my antibodies recognizes only one isoform (the shorter protein) instead of two though the immunogen covers the opposite end of SGLT1 sequence (C-terminus) presents in both isoforms. For now, I'm pretty confused what protein I see in my WB.
1. We have two epitope specific antibodies to SGLT1. The antibody SGLT-101AP is a N-epitope (with respect to the other epitope) and SGLT-112AP is a C-epitope downstream from the SGLT-101AP. Both antibodies were tested on intestinal brush boarder membranes (BBM) and appears to label the high MW isoform of SGLT1. We did not see the smaller variant of SGLT1 in BBM. It is possible that smaller variant is abundant in breast cancer cells and high MW variant is not expressed in these cells. These observations yet to be confirmed using PC-SGLT1 samples or using recombinant SGLT1 proteins and compare them with renal BBM and intestinal BBM samples.

Customer ratings for SGLT1 Antibody

Number of ratings: 1
Average rating: 5

Excellent
from Verified Customer on 12/11/2020

Excellent results in Immunohistochemistry using mouse kidney tissue. 1/350 primary antibody dilution for 16 hours.

All incoming ratings are posted by actual shop visitors / webshop customers and their content is checked by the operator by means of a visual inspection.

Accessories

Product

Note

Status

Price

SGLT1 Antibody FITC

SGLT.c-FITC

$375.00 *

SGLT1 Antibody BIOTIN

SGLT.c-BIOTIN

$375.00 *

SGLT1 Blocking Peptide

P-SGLT.c

$155.00 *

SGLT1 Positive Control

PC-SGLT

$200.00 *

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SGLT-101AP

SGLT1 Antibody

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SGLT2-201AP

SGLT2 Antibody

$285.00 *

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SGLT1 Antibody

Product no.: SGLT-101AP

$285.00

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General Information
Product	SGLT1 Antibody
Description	Affinity Purified Sodum/glucose cotransporter 1 Antibody Mid-Region
Verified Applications	CM, Dot Blot, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human
Immunogen	Synthetic peptide corresponding to amino acids 402-422 of Human SGLT1.
Accession #	NCBI: NP_000334.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.6 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	Mid-Region
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:4,000
ELISA	1:4,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:200
Western Blot	1:500-1:1,000
Protein
Uniprot #	P13866
Overview	Actively transports glucose into cells by Na+ cotransport with a Na+ to glucose coupling ratio of 2:1.
Molecular Function	glucose:sodium symporter activity
Subcellular Location	Membrane
Expression	Expessed mainly in intestine and kidney.
Structure	Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Alternative Nomenclature	D22S675 antibody High affinity sodium glucose cotransporter 1 antibody Human Na+/glucose cotransporter 1 antibody NAGT antibody SGLT 1 antibody SLC5A1 antibody Sodium glucose cotransporter 1 antibody Solute carrier family 5 member 1 antibody

Protein information supplied by Uniprot

To view the Specification Sheet for SGLT1 Antibody, please click the link below:

SGLT1 Antibody | FabGennix

A customer wants to know that this product is specific to SGLT1 (That is not reacted with SGLT2). Is this product tested in being specific to SGLT1? If you have tested data, please send me. Or if not data, could you tell me something proving to be specific to SGLT1?
We would like to know whether SGLT1 Positive Control (PC-SGLT) are developed from insect sources or baculovirus expression systems.
I'm currently working on 5 different breast cancer cell lines and I'm using yours standard protocol for WB in that particular Antibodies, with 40ug of protein instead 60 (I have less nonspecific bands). My WB shows the band at approx. 58kDa in each cell line. I've checked UniProt database for SGLT1 and indeed there are 2 isoforms of this protein. The difference is about 120aas on the beginning of the protein sequence. I'm just curious why my antibodies recognizes only one isoform (the shorter protein) instead of two though the immunogen covers the opposite end of SGLT1 sequence (C-terminus) presents in both isoforms. For now, I'm pretty confused what protein I see in my WB.

A customer wants to know that this product is specific to SGLT1 (That is not reacted with SGLT2). Is this product tested in being specific to SGLT1? If you have tested data, please send me. Or if not data, could you tell me something proving to be specific to SGLT1?
1. Our SGLT1 antibodies, SGLT-101AP and SGLT-112AP, are epitope specific antibodies. They are raised against synthetic peptides that are unique to the SGLT1 protein and are not present in SGLT2. These antibodies do not react to random peptide in ELISA and in Dot Blot assays. These antibodies are affinity purified on immobilized antigen based affinity chromatography. Based on these observations, they will not cross react with SGLT2 proteins.
We would like to know whether SGLT1 Positive Control (PC-SGLT) are developed from insect sources or baculovirus expression systems.
1. SGLT1 Positive Control (PC-SGLT1.m and PC-SGLT.C) are not obtained from insect sells, SF9 cells or other cells. It is a partially purified protein obtained from normal enterocytes. The preparation is in SDS-Sample buffer and does not contain any preservatives. The shelf life is 1 year at -20⁰C and 6 months at 4⁰C.
I'm currently working on 5 different breast cancer cell lines and I'm using yours standard protocol for WB in that particular Antibodies, with 40ug of protein instead 60 (I have less nonspecific bands). My WB shows the band at approx. 58kDa in each cell line. I've checked UniProt database for SGLT1 and indeed there are 2 isoforms of this protein. The difference is about 120aas on the beginning of the protein sequence. I'm just curious why my antibodies recognizes only one isoform (the shorter protein) instead of two though the immunogen covers the opposite end of SGLT1 sequence (C-terminus) presents in both isoforms. For now, I'm pretty confused what protein I see in my WB.
1. We have two epitope specific antibodies to SGLT1. The antibody SGLT-101AP is a N-epitope (with respect to the other epitope) and SGLT-112AP is a C-epitope downstream from the SGLT-101AP. Both antibodies were tested on intestinal brush boarder membranes (BBM) and appears to label the high MW isoform of SGLT1. We did not see the smaller variant of SGLT1 in BBM. It is possible that smaller variant is abundant in breast cancer cells and high MW variant is not expressed in these cells. These observations yet to be confirmed using PC-SGLT1 samples or using recombinant SGLT1 proteins and compare them with renal BBM and intestinal BBM samples.

Customer ratings for SGLT1 Antibody

Number of ratings: 1
Average rating: 5

Excellent
from Verified Customer on 12/10/2020

Excellent staining in Western blot using mouse tissue lysate- whole (cortex). 1/1,000 primary antibody dilution in .1% BSA.

All incoming ratings are posted by actual shop visitors / webshop customers and their content is checked by the operator by means of a visual inspection.

Accessories

Product

Note

Status

Price

SGLT1 Antibody FITC

SGLT.m-FITC

$375.00 *

SGLT1 Antibody BIOTIN

SGLT.m-BIOTIN

$375.00 *

SGLT1 Blocking Peptide

P-SGLT.m

$155.00 *

SGLT1 Positive Control

PC-SGLT

$200.00 *

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We also recommend

SGLT2-201AP

SGLT2 Antibody

$285.00 *

SGLT-112AP

SGLT1 Antibody

$285.00 *

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ENT1 Antibody

Product no.: ENT1-101AP

$285.00

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Description

General Information
Product	ENT1 Antibody
Description	Affinity Purified Equilibrative nitrobenzylthioinosine-sensitive nucleoside transporter ENT1 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid reigon 410-460 on mouse Equilibrative nitrobenzylthioinosine-sensitive nucleoside transporter ENT1 protein.
Accession #	GenBank: AAF64035.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9JIM1
Overview	Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).
Molecular Function	Nucleoside transmembrane transporter activity
Subcellular Location	Cell membrane; Basolateral cell membrane; Apical cell membrane
Expression	Highly expressed in heart, spleen, lung, liver and testis. Lower level of expression in brain and kidney.
Structure	Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
Alternative Nomenclature	Equilibrative NBMPR-sensitive nucleoside transporter antibody Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody Equilibrative nucleoside transporter 1 antibody es-type antibody Nucleoside transporter, es-type antibody Slc29a1 antibody solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody Solute carrier family 29 member 1 antibody

Protein information supplied by UniProt

Accessories

Product

Note

Status

Price

ENT1 Antibody BIOTIN

ENT1-BIOTIN

$375.00 *

ENT1 Antibody FITC

ENT1-FITC

$375.00 *

ENT1 Blocking Peptide

P-ENT1

$155.00 *

ENT1 Positive Control

PC-ENT1

$200.00 *

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We also recommend

ENT3-301AP

SLC29A3 Antibody

$285.00 *

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DWORF Antibody

Product no.: DWORF-101AP

$285.00

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Description

General Information
Product	DWORF Antibody
Description	Affinity Purified Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Mouse
Immunogen	Synthetic peptide taken within amino acid region 1-30 on mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P0DN83
Overview	Enhances the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by displacing ATP2A1/SERCA1 inhibitors, thereby acting as a key regulator of skeletal muscle activity. Does not directly stimulates SERCA pump activity. Enhances sarcoplasmic reticulum Ca2+ uptake and myocyte contractility by displacing the SERCA inhibitory peptides sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN).
Subcellular Location	Sarcoplasmic reticulum membrane; Single-pass membrane protein
Expression	Highly expressed in heart (at protein level). Detected in heart and soleus, a postural muscle group of the hindlimb containing the highest enrichment of slow-twitch muscle fibers. Also expressed in diaphragm, which contains some slow-twitch fibers. Not detected in the quadriceps, a fast-twitch muscle group, or in cardiac atrial muscle. Not expressed in the prenatal heart but gradually increases in abundance postnatally.
Alternative Nomenclature	Dwarf open reading frame antibody DWORF antibody Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF SERCA regulator DWORF antibody

Protein information supplied by UniProt

Accessories

Product

Note

Status

Price

DWORF Antibody BIOTIN

DWORF-BIOTIN

$375.00 *

DWORF Antibody FITC

DWORF-FITC

$375.00 *

DWORF Blocking Peptide

P-DWORF

$155.00 *

DWORF Positive Control

PC-DWORF

$200.00 *

Shipping Options

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We also recommend

DWORF-112AP

DWORF Antibody

$285.00 *

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Browse these categories as well: Calcium Binding, Plasma Membrane, ATPases, Metabolism, Metabolism & Homeostasis, Cardiology, Primaries

DWORF Antibody

Product no.: DWORF-112AP

$285.00

Shipping Options

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Description

General Information
Product	DWORF Antibody
Description	Affinity Purified Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synththetic peptide corresponding to full length Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5-1.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Purification Method	Immobilized antigen affinity chromatography
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:50,000
Immunoprecipitation	1:200
Western Blot	1:2,500-1:5,000
Protein
Uniprot #	P0DN83
Overview	Enhances the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by displacing ATP2A1/SERCA1 inhibitors, thereby acting as a key regulator of skeletal muscle activity. Does not directly stimulates SERCA pump activity. Enhances sarcoplasmic reticulum Ca2+ uptake and myocyte contractility by displacing the SERCA inhibitory peptides sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN).
Molecular Function	Sarcoplasmic reticulum membrane; Single-pass membrane protein
Alternative Nomenclature	Dwarf open reading frame antibody DWORF antibody Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORFSERCA regulator DWORF antibody

Protein information supplied by UniProt.