General Information

Product

VGluT1 Antibody

Description

Affinity Purified Vesicular Glutamate transporter 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on mouse Vesicular Glutamate transporter 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q3TXX4

Overview

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Molecular Function

extracellularly glutamate-gated chloride channel activity

inorganic phosphate transmembrane transporter activity

sodium:inorganic phosphate symporter activity

sodium-dependent phosphate transmembrane transporter activity

Subcellular Location

synaptic vesicle membrane

Membrane  Curated; Multi-pass membrane protein 

synaptosome

Expression

Expressed in hippocampus (at protein level). Expressed in the molecular layer of the cerebellum and in retina.

Structure

Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Alternative Nomenclature

BNPI antibody

Brain specific Na (+) dependent inorganic phosphate cotransporter antibody

Slc17a7 antibody

Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody

Solute carrier family 17 member 7 antibody

Vesicular glutamate transporter 1 antibody

VGluT1 antibody

General Information

Product

Bruchpilot Antibody

Description

Affinity Purified Bruchpilot Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 150-200 on D. melanogaster (fruit fly) Bruchpilot protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

A0A0B4K7K9

Expression

cytoskeleton of presynaptic active zone

Other locations

neuromuscular junction

presynaptic active zone

Structure

Coiled coil

Alternative Nomenclature

BRP

Bruchpilot

Cast

General Information

Product

Phospho-Bruchpilot Antibody

Description

Affinity Purified Phosphorylated Bruchpilot Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Phosphorylated synthetic peptide taken within amino acid region 150-200 on D. melanogaster (fruit fly) Bruchpilot protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

A0A0B4K7K9

Expression

cytoskeleton of presynaptic active zone

Other locations

neuromuscular junction

presynaptic active zone

Structure

Coiled coil

Alternative Nomenclature

BRP

Bruchpilot

Cast

General Information

Product

PARD6B Antibody

Description

Affinity Purified Partitioning Defective 6 homolog beta Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 300-350 on human Partitioning Defective 6 homolog beta.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9BYG5

Overview

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Molecular Function

protein kinase C binding

Rho GTPase binding

Subcellular Location

Cell membrane, Cytoplasm

Expression

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Structure

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Alternative Nomenclature

OTTHUMP00000031269 antibody

PAR 6 beta antibody

Par 6 partitioning defective 6 homolog beta (C. elegans) antibody

PAR6B antibody

PARD6 beta antibody

PARD6B antibody

partitioning defective 6 homolog beta antibody

partitioning-defective protein 6, C. elegans, homolog of, beta antibody

General Information

Product

PARD6B Antibody

Description

Affinity Purified Partitioning Defective 6 homolog beta Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 340- 372 on human Partitioning Defective 6 homolog beta.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9BYG5

Overview

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Molecular Function

protein kinase C binding

Rho GTPase binding

Subcellular Location

Cell membrane, Cytoplasm

Expression

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Structure

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Alternative Nomenclature

OTTHUMP00000031269 antibody

PAR 6 beta antibody

Par 6 partitioning defective 6 homolog beta (C. elegans) antibody

PAR6B antibody

PARD6 beta antibody

PARD6B antibody

partitioning defective 6 homolog beta antibody

partitioning-defective protein 6, C. elegans, homolog of, beta antibody

General Information

Product

NHERF-1 Antibody

Description

Affinity Purified Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 255-305 on human Na(+)/H(+) exchange regulatory cofactor NHE-RF1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

O14745

Overview

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.

Molecular Function

beta-2 adrenergic receptor binding

beta-catenin binding

chloride channel regulator activity

dopamine receptor binding

gamma-aminobutyric acid transmembrane transporter activity

growth factor receptor binding

myosin II binding

PDZ domain binding

pho

Subcellular Location

Plasma membrane, cytoplasm

Expression

Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

Alternative Nomenclature

EBP 50 antibody

Ezrin radixin moesin binding phosphoprotein 50 antibody

Na(+)/H(+) exchange regulatory cofactor NHE RF antibody

Na+/H+ exchange regulatory co factor antibody

NHERF 1 antibody

NHERF antibody

NHRF1_HUMAN antibody

NPHLOP2 antibody

Regulatory cofactor of Na(+)/H(+) exchanger antibody

Slc9a3r1 antibody

Sodium hydrogen exchanger regulatory factor 1 antibody

Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody

Solute carrier family 9 isoform 3 regulatory factor 1 antibody

General Information

Product

Phospho-Cofilin Antibody

Description

Affinity Purified Phosphorylated Cofilin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 1-50 on human Phosphorylated Cofilin.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P23528

Overview

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity).

Molecular Function

actin filament binding

Subcellular Location

Plasma membrane, Cytoskeleton, Nucleus

Expression

Widely distributed in various tissues.

Structure

Belongs to the actin-binding proteins ADF family.

Alternative Nomenclature

18 kDa phosphoprotein antibody

CFL 1 antibody

CFL antibody

CFL1 antibody

COF1_HUMAN antibody

Cofilin 1 antibody

Cofilin 1 non muscle antibody

Cofilin antibody

Cofilin non muscle isoform antibody

Cofilin-1 antibody

epididymis secretory protein Li 15 antibody

HEL-S-15 antibody

non-muscle isoform antibody

p18 antibody

General Information

Product

BMP2 Antibody

Description

Affinity Purified Bone morphogenetic Protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 340-396 on human Bone morphogenetic Protein 2.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P12643

Overview

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).

Molecular Function

BMP receptor binding

co-receptor binding

cytokine activity

growth factor activity

phosphatase activator activity

retinol dehydrogenase activity

signaling receptor binding

SMAD binding

Subcellular Location

Secreted

Expression

Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Structure

Belongs to the TGF-beta family.

Alternative Nomenclature

BDA2 antibod

BMP 2 antibody

BMP 2A antibody

BMP-2 antibody

BMP-2A antibody

Bmp2 antibody

BMP2_HUMAN antibody

BMP2A antibody

Bone morphogenetic protein 2 antibody

Bone morphogenetic protein 2A antibody