General Information
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Product
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MYLK Positive Control
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Description
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Western Blot Positive Control Myosin light chain kinase, smooth muscle isoform 1
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Verified Applications
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WB
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Immunogen
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Purified Protein
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Accession #
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NCBI: NP_444253.3
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Physical Properties
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Quantity
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5 Applications
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Volume
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Inquire
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Form
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Western Blot Positive Control
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Storage
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-20⁰C for long term storage
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Application Protocol
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Western Blot
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1:500
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Protein
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Uniprot #
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Q15746
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Overview
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Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma.
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Molecular Function
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Kinase, Serine/threonine-protein kinase, Transferase
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Subcellular Location
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Cytoplasm, Cell projection > lamellipodium, Cleavage furrow, Cytoplasm > cytoskeleton
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Expression
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Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).
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Structure
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All isoforms including Telokin bind calmodulin. Interacts with SVIL. Interacts with CTTN; this interaction is reduced during thrombin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Binds to NAA10/ARD1 and PTK2B/PYK2.
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Alternative Nomenclature
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caMLCK antibody
cardiac MLCK antibody
Cardiac-MyBP-C-associated Ca/CaM kinase antibody
MLC kinase antibody
MLCK antibody
MLCK2 antibody
Mylk3 antibody
Myosin light chain kinase 3 antibody
Putative myosin light chain kinase 3 antibody
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