General Information

Product

B-raf Antibody

Description

Affinity Purified Serine/threonine-protein kinase B-raf Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein.

Accession #

NCBI:  NP_004324.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500. Molecular Weight: 84.4

Protein

Uniprot #

P15056

Overview

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Nucleus, Cytoplasm, Cell membrane

Expression

Brain and testis.

Structure

Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.

Alternative Nomenclature

94 kDa B raf protein antibody

B raf 1 antibody

B Raf proto oncogene serine threonine protein kinase antibody

B RAF1 antibody

B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody

BRAF 1 antibody

cRmil antibody

Murine sarcoma viral v raf oncogene homolog B1 antibody

NS7 antibody

Oncogen BRAF antibody

p94 antibody

Proto-oncogene B-Raf antibody

Proto-oncogene c-Rmil antibody

RAFB1 antibody

RMIL antibody

Serine/threonine-protein kinase B-raf antibody

v-Raf murine sarcoma viral oncogene homolog B1 antibody

General Information

Product

SQSTM1 / p62 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-440 on human Sequestosome-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13501

Overview

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

Molecular Function

identical protein binding, protein kinase binding, protein kinase C binding, protein serine/threonine kinase activity, receptor tyrosine kinase binding, SH2 domain binding, ubiquitin binding, ubiquitin protein ligase binding, zinc ion binding

Subcellular Location

Cytoplasm

Late endosome

Lysosome

Cytoplasmic vesicle › autophagosome

Nucleus

Endoplasmic reticulum

Cytoplasm › P-body

Expression

Ubiquitously expressed.

Structure

Homooligomer or heterooligomer; may form homotypic arrays. Dimerization interferes with ubiquitin binding.

Alternative Nomenclature

A170 

EBI 3 associated protein of 60 kDa 

EBIAP 

FTDALS3 

ORCA 

OSF-6 

Osi 

OSIL 

Oxidative stress induced like 

p60 

p62 

p62B 

Paget disease of bone 3 

PDB3 

Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa 

PKC-zeta-interacting protein 

Protein kinase C-zeta-interacting protein 

Sequestosome 1 

SQSTM 1 

STAP 

STONE14 

Ubiquitin-binding protein p62 

ZIP 

ZIP3 

General Information

Product

CCAR1 Antibody

Description

Affinity Purified Cell division cycle and apoptosis regulator protein 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 645-695 on human Cell division cycle and apoptosis regulator protein 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q8IX12

Overview

Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (PubMed:12816952). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).

Molecular Function

Activator, Repressor

Subcellular Location

Cytoplasm > perinuclear region

Expression

Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.

Structure

Contains 1 SAP domain. Coiled coil.

Alternative Nomenclature

Carp1 antibody
Ccar1 antibody
Cell division cycle and apoptosis regulator protein 1 antibody
Death inducer with SAP domain antibody
RP11-437A18.1 antibody

General Information

Product

MAPK2 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 2.

Accession #

NCBI:  NP_116584

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P49137

Overview

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

Expressed in all tissues examined.

Structure

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative Nomenclature

ERK 2 antibody

ERT1 antibody
Extracellular Signal Regulated Kinase 2 antibody

MAP kinase 2 antibody

MAPK 2 antibody
Mitogen-activated protein kinase 2 antibody
protein kinase, mitogen-activated, 2 antibody
protein tyrosine kinase ERK2 antibody

General Information

Product

Lamin B1 antibody - Nuclear Envelope Marker Antibody

Description

Affinity Purified B1 antibody - Nuclear Envelope Marker Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Lamin-B1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P20700

Overview

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Molecular Function

phospholipase binding

structural molecule activity

Subcellular Location

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Expression

Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12.

Structure

Belongs to the intermediate filament family.

Alternative Nomenclature

ADLD antibody

lamin B1 antibody

LMN antibody

LMN2 antibody

LMNB antibody

Lmnb1 antibody

General Information

Product

ZIP Kinase Antibody

Description

Affinity Purified Death-associated protein kinase 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region on 400-448 on mouse Death-associated protein kinase 3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

O54784

Overview

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization.

Molecular Function

ATP binding

identical protein binding

kinase activity

leucine zipper domain binding

protein C-terminus binding

protein homodimerization activity

protein serine/threonine kinase activity

Rho GTPase binding

Subcellular Location

Cytoskeleton, Nucleus

Expression

Highly expressed in heart, brain, lung, skeletal muscle, kidney and testis. Lower levels in liver and spleen.

Structure

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.

Alternative Nomenclature

DAP kinase 3 antibody

DAP like kinase antibody

DAPK3 antibody

Death associated kinase 3 antibody

Death associated protein kinase 3 antibody

Dlk antibody

MYPT1 kinase antibody

ZIP antibody

ZIP kinase antibody

ZIP kinase isoform antibody

ZIPK antibody

zipper-interacting protein kinase antibody

General Information

Product

IFI6 Antibody

Description

Affinity Purified Interferon alpha-inducible protein 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide take within amino acid region 80-130 on human Interferon alpha-inducible protein 6.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P09912

Molecular Function

Up-regulated by type-I interferon (PubMed:14728724, PubMed:17823654). Up-regulated upon Dengue virus type 2/DENV2 infection (at protein level) (PubMed:26244642).

Subcellular Location

Mitochondrion

Expression

Up-regulated by type-I interferon (PubMed:14728724, PubMed:17823654). Up-regulated upon Dengue virus type 2/DENV2 infection (at protein level) (PubMed:26244642).

Structure

Belongs to the IFI6/IFI27 family.

Alternative Nomenclature

Ifi-6-16 antibody
IFI6 antibody
IFI6_HUMAN antibody
Interferon alpha-inducible protein 6 antibody
Interferon-induced protein 6-16 antibody

General Information

Product

MLKL Antibody

Description

Affinity Purified Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 400-450 on mouse Mixed lineage kinase domain-like protein MLKL.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9D2Y4

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414). Within this complex, may play a role in the proper targeti

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody

General Information

Product

Phospho-MLKL Antibody

Description

Affinity Purified Phosphorylated Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 350-400 on human Mixed lineage kinase domain-like protein MLKL

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q8NB16

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414).

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody