General Information

Product

TIE2 Antibody

Description

Affinity Purified Tyrosine-protein kinase receptor Tie-1 isoform 2 Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 540-590 on human Tyrosine-protein kinase receptor Tie-1 isoform 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02763

Overview

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Integral component of membrane

Expression

Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family.  Contains protein kinase domain.

Alternative Nomenclature

Angiopoietin 1 receptor antibody

CD202b antibody

Endothelial tyrosine kinase antibody

Endothelium specific receptor tyrosine kinase 2 antibody

Hyk antibody

p140 TEK antibody

TEK antibody

tek tyrosine kinase antibody

TIE 2 antibody

Tunica interna endothelial cell kinase antibody

Tyrosine kinase with Ig and EGF homology domains 2 antibody

Tyrosine protein kinase receptor TEK antibody

Tyrosine protein kinase receptor TIE 2 antibody

Venous malformations multiple cutaneous and mucosal antibody

VMCM 1 antibody

VMCM antibody

General Information

Product

Myeloperoxidase Antibody

Description

Affinity Purified Myeloperoxidase Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 290-340 human Myeloperoxidase protein.

Accession #

GenBank: AAB25582.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P05164

Overview

Is a component of the host defense system of polymorphonuclear leukocytes.  Is responsible for the microbicidal activity against a range of organisms.

Molecular Function

Oxireductase, Peroxidase, chromatin binding

Alternative Nomenclature

84 kDa myeloperoxidase antibody

MPO antibody

Myeloperoxidase heavy chain antibody

Myeloperoxidase light chain antibody

General Information

Product

Myeloperoxidase Antibody Multi-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Multi-epitope antibody containing a mixture of synthetic peptides taken within amino acid region 290-340 and 600-650 of human Myeloperoxidase protein.

Accession #

GenBank: AAB25582.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.88-1.03 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multiple epitopes

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P05164

Overview

Is a component of the host defense system of polymorphonuclear leukocytes.  Is responsible for the microbicidal activity against a range of organisms.

Molecular Function

Oxireductase, Peroxidase, chromatin binding

Structure

Homodimer; disulfide linked.  Each monomer contains a light chain and heavy chain

Alternative Nomenclature

84 kDa myeloperoxidase antibody

MPO antibody

Myeloperoxidase heavy chain antibody

Myeloperoxidase light chain antibody

General Information

Product

Myeloperoxidase Antibody Mid-Region

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 580-630 human Myeloperoxidase protein.

Accession #

GenBank: AAB25582.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P05164

Overview

Is a component of the host defense system of polymorphonuclear leukocytes.  Is responsible for the microbicidal activity against a range of organisms.

Molecular Function

Oxireductase, Peroxidase, chromatin binding

Structure

Homodimer; disulfide linked.  Each monomer contains a light chain and heavy chain

Alternative Nomenclature

84 kDa myeloperoxidase antibody

MPO antibody

Myeloperoxidase heavy chain antibody

Myeloperoxidase light chain antibody

General Information

Product

Myeloperoxidase Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 600-650 human Myeloperoxidase protein.

Accession #

GenBank: AAB25582.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.88-1.03 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P05164

Overview

Is a component of the host defense system of polymorphonuclear leukocytes.  Is responsible for the microbicidal activity against a range of organisms.

Molecular Function

Oxireductase, Peroxidase, chromatin binding

Structure

Homodimer; disulfide linked.  Each monomer contains a light chain and heavy chain

Alternative Nomenclature

84 kDa myeloperoxidase antibody

89 kDa myeloperoxidase antibody

EC 1.11.1.7 antibody

EC1.11.2.2 antibody

MPO antibody

MPO antibody

Myeloperoxidase heavy chain antibody

Myeloperoxidase light chain antibody

PERM_HUMAN antibody

General Information

Product

Myeloperoxidase Antibody C-terminal

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-745 human Myeloperoxidase protein.

Accession #

GenBank: AAB25582.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.88-1.03 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P05164

Overview

Is a component of the host defense system of polymorphonuclear leukocytes.  Is responsible for the microbicidal activity against a range of organisms.

Molecular Function

Oxireductase, Peroxidase, chromatin binding

Structure

Homodimer; disulfide linked.  Each monomer contains a light chain and heavy chain

Alternative Nomenclature

84 kDa myeloperoxidase antibody

MPO antibody

Myeloperoxidase heavy chain antibody

Myeloperoxidase light chain antibody

General Information

Product

CD163 Antibody

Description

Affinity Purified CD163 Antibody N-epitope

Verified Applications

ELISA, IHC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 600-650  on human CD163 protein.

Accession #

GenBank: EAW88662.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunohistochemistry

1:150

Western Blot

1:500

Protein

Uniprot #

D3DUU0

Overview

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Molecular Function

Scavenger receptor activity

Subcellular Location

Membrane

Expression

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

Structure

Interacts with CSNK2B.

Alternative Nomenclature

CD163 antigen antibody

CD163 molecule antibody

Hemoglobin Scavenger Receptor antibody

Macrophage associated antigen antibody

MM130 antibody

Scavenger receptor cysteine rich type 1 protein M130 antibody

sCD163 antibody

Soluble CD163 antibody

General Information

Product

CD163 Antibody C-epitope

Verified Applications

ELISA, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region  887-905 on human CD163 protein.

Accession #

GenBank: EAW88662.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

D3DUU0

Overview

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Molecular Function

Scavenger receptor activity

Subcellular Location

Membrane

Expression

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

Structure

Interacts with CSNK2B.

Alternative Nomenclature

CD163 antigen antibody

CD163 molecule antibody

Hemoglobin Scavenger Receptor antibody

Macrophage associated antigen antibody

MM130 antibody

Scavenger receptor cysteine rich type 1 protein M130 antibody

sCD163 antibody

Soluble CD163 antibody

General Information

Product

Calponin Antibody

Description

Affinity Purified Calponin 1, basic, smooth muscle Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 120-170 on human Calponin 1, basic, smooth muscle protein.

Accession #

NCBI:  XP_005259798.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

B7Z7E1

Overview

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Molecular Function

Actin-Binding; actomyosin structure organization

Subcellular Location

Cytoskeleton; Focal Adhesion

Expression

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Structure

Belongs to the calponin family.UniRule annotation

Contains 1 CH (calponin-homology) domain.

Contains 3 calponin-like repeats.

Alternative Nomenclature

Basic calponin antibody

Calponin 1 antibody

Calponin 1 basic smooth muscle antibody

Calponin H1 antibody

Calponin H1 smooth muscle antibody

Calponins basic antibody

CNN 1 antibody

Sm Calp antibody

SMCC antibody

smooth muscle antibody

General Information

Product

Calponin Antibody

Description

Affinity Purified Calponin 1, basic, smooth muscle Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-247 on human calponin 1, basic, smooth muscle protein.

Accession #

NCBI:  XP_005259798.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

B7Z7E1

Overview

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Molecular Function

Actin-Binding; actomyosin structure organization

Subcellular Location

Cytoskeleton; Focal Adhesion

Expression

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Structure

Belongs to the calponin family.UniRule annotation

Contains 1 CH (calponin-homology) domain.

Contains 3 calponin-like repeats.

Alternative Nomenclature

Basic calponin antibody

Calponin 1 antibody

Calponin 1 basic smooth muscle antibody

Calponin H1 antibody

Calponin H1 smooth muscle antibody

Calponins basic antibody

CNN 1 antibody

Sm Calp antibody

SMCC antibody

smooth muscle antibody