General Information
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Product
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CHMP2B Antibody FITC-Conjugated
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 200-250 on human Vacuolar-sorting Protein SNF8 protein.
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Accession #
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NCBI: NP_009172
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5-1.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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FITC-Conjugated
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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Immunoprecipitation
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1:200
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Western Blot
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1:2,500-1:5,000
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Protein
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Uniprot #
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Q96H20
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Overview
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Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
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Molecular Function
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channel regulator activity
protein C-terminus binding
protein homodimerization activity
protein N-terminus binding
transcription factor binding
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Subcellular Location
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Endosome membrane
Late endosome membrane
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Expression
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Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS25 and VPS36. SNF8 is essential for the stability of the ESCRT-II complex. ESCRT-II interacts with ELL. Interacts with TSG101 (via the C-terminal domain). Interacts with RILPL1 (via the N-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with 14-3-3 proteins.
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Structure
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Belongs to the SNF8 family.
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Alternative Nomenclature
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ALS17 antibody
Charged multivesicular body protein 2b antibody
CHM2B_HUMAN antibody
CHMP family, member 2B antibody
CHMP2.5 antibody
CHMP2b antibody
Chromatin modifying protein 2b antibody
DMT1 antibody
hVps2-2 antibody
Vacuolar protein sorting 2, yeast, homolog of, B antibody
Vacuolar protein sorting 2-2 antibody
Vacuolar protein sorting-associated protein 2-2 antibody
VPS2 homolog B antibody
Vps2-2 antibody
VPS2B antibody
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