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ARF1 Antibody

Product no.: ARF1-101AP

$320.00

General Information
Product	ARF1 Antibody
Description	Affinity Purified ADP-ribosylation factor 1 Antibody
Verified Applications	ELISA, ICC/IF, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity.
Specificity	does not cross react to other members of the Ras family or to other ARF members. This antibody will label ARF isoform 1, 2, 3, and 4.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.58-0.65 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:100
Immunocytochemistry	Use at an assay dependent concentration.
Western Blot	1:500. Predicted Molecular Weight 21 kDa
Protein
Uniprot #	P84079
Overview	GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.
Molecular Function	ADP-ribosyltransferase; GTP binding; ER-Golgi transport
Subcellular Location	Gogli apparatus
Expression	Cell junction; Membrane; Synapse
Structure	Belongs to the small GTPase superfamily, Arf family
Alternative Nomenclature	DJ133P16.1 antibody

Protein information supplied by UniProt

Fabgennix ARF1 Antibody has been used in 1 Publication

Lin, Lin et al. “The Antibiotic Gentamicin Inhibits Specific Protein Trafficking Functions of the Arf1/2 Family of GTPases.” Antimicrobial Agents and Chemotherapy 55.1 (2011): 246–254. PMC3019663

Western Blot; Rat

Publishing research using ARF1-101AP? Please let us know, we can cite the reference in this datasheet.

Customer ratings for ARF1 Antibody

Number of ratings: 2
Average rating: 4.5

Good
from Verified Customer on 12/11/2020

Immunoprecipitation preformed using rat whole brain tissue lysate. 1000 ug total protein input. 3.5 ul/mg lysate incubated for 17 hours at 4C. Able to get the antibody to work well for tissue lysate, but was unable to work in ell lysates.

Excellent
from Anonymous on 11/14/2015

Immunocytochemistry/Immunofluorescence
Human Cell (Neuroblastoma) Paraformaldehyde
Permeabilization-15 mins in 0.01% Triton X
Serum as blocking agent for 30 minutes
1:100 antibody dilution for 2 hours at 20C

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Accessories

Product

Note

Status

Price

ARF1 Antibody FITC

ARF1-FITC

$375.00 *

ARF1 Antibody BIOTIN

ARF1-BIOTIN

$375.00 *

ARF1 Blocking Peptide

P-ARF1

$155.00 *

ARF1 Positive Control

PC-ARF1

$200.00 *

General Information
Product	ARF4 Antibody
Description	Affinity Purified ADP-ribosylation factor 4 Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on ARF4 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.71-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P61751
Overview	GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.
Molecular Function	ADP-ribosyltransferase; GTP binding; ER-Golgi transport
Subcellular Location	Gogli apparatus
Expression	Cell junction; Membrane; Synapse
Structure	Belongs to the small GTPase superfamily, Arf family
Alternative Nomenclature	ADP ribosylation factor 2 antibody ADP ribosylation factor 4 antibody

General Information
Product	C6orf150 Antibody
Description	Affinity Purified Cyclic GMP-AMP synthase Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 450-500 on mouse cGAS protein.
Accession #	NCBI: NP_775562
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.60 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:100
Immunoprecipitation	1:150
Western Blot	1:500
Protein
Uniprot #	Q8C6L5
Overview	Catalyzes the formation of cyclic GMP-AMP from ATP and GTP. Has antiviral activity through acting as a cystolic DNA senser.
Molecular Function	Nucleotidyltransferase; ATP binding; DNA binding
Subcellular Location	Cytoplasm; Cytosol
Expression	Is expressed in the monocytic cell line THP1 by type 1 Interferons
Structure	Monomer when in absence of DNA. Homodimer when bound to DNA.
Alternative Nomenclature	C6orf150 antibody cGAMP synthase antibody cGAS antibody Chromosome 6 open reading frame 150 antibody Cyclic GMP-AMP synthase antibody h-cGAS antibody Hypothetical protein LOC115004 antibody Mab 21 domain containing 1 antibody MB21D1 antibody protein MB21D1 antibody Uncharacterized protein C6orf150 antibody

General Information
Product	N-Chimaerin Antibody
Description	Affinity Purified N-Chimaerin isoform 1 Antibody
Verified Applications	ELISA, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on alpha 2 Chimaerin protein.
Accession #	NCBI: NP_001813
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.58-0.63 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Western Blot	1:500
Protein
Uniprot #	P15882
Overview	GTPase-activating protein for p21-rac and a phorbol ester receptor. Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance.
Molecular Function	GTPase activation
Subcellular Location	Cytosol
Expression	In neurons in brain regions that are involved in learning and memory processes.
Structure	Interacts with EPHA4; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation.
Alternative Nomenclature	A-chimaerin antibody Alpha-chimerin antibody ARHGAP2 antibody CHN antibody Chn1 antibody N chimaerin antibody NC antibody Rho GTPase-activating protein 2 antibody RHOGAP2 antibody

General Information
Product	Chimaerin beta 2 Antibody
Description	Affinity Purified Beta-chimaerin Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 150-180 on beta 2-chimaerin protein.
Accession #	NCBI: NM_004067
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.68-0.71 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P52757
Overview	GTPase-activating protein for p21-rac. May play role in progression from low-grade to high-grade tumors.
Molecular Function	Metal binding; Zinc
Subcellular Location	Membrane
Expression	Brain, pancreas, heart, placenta. Weakly expressed in kidney and liver.
Structure	Contains 1 phorbol-ester/DAG-type zinc finger; 1 Rho-GAP domain; 1 SH2 domain.
Alternative Nomenclature	ARHGAP3 antibody BCH antibody CHN2 antibody MGC138360 antibody Rho GTPase activating protein 3 antibody RHOGAP3 antibody

General Information
Product	Chimaerin beta 3 Antibody
Description	Affinity Purified Beta-Chimaerin isoform 3 Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 1-100 on human beta-chimaerin isoform 3 protein.
Accession #	NCBI: NP_001279998.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.68-0.71 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P52757
Overview	GTPase-activating protein for p21-rac. May play role in progression from low-grade to high-grade tumors.
Molecular Function	Metal binding; Zinc
Subcellular Location	Membrane
Expression	Brain, pancreas, heart, placenta. Weakly expressed in kidney and liver.
Structure	Contains 1 phorbol-ester/DAG-type zinc finger; 1 Rho-GAP domain; 1 SH2 domain.
Alternative Nomenclature	ARHGAP3 antibody BCH antibody Beta chimerin antibody Chimaerin3 antibody CHN3 antibody MGC138360 antibody Rho GTPase activating protein 3 antibody RHOGAP3 antibody

General Information
Product	EHD2 Antibody
Description	Affinity Purified EH domain-containing protein 2 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse Rat
Immunogen	Synthetic peptide taken within amino acid 420-450 on human EH-Domain Containing Protein 2.
Accession #	NCBI: NP_055416.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9NZN4
Overview	Plays a role in membrane reorganization in response to nucleotide hydrolysis
Molecular Function	ATP binding; GTPase activity; nucleic acid binding; calcium ion binding; GTP binding
Subcellular Location	Cell membrane, Endosome, Membrane
Expression	Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.
Structure	Homodimer and homooligomer. Interacts with EHD1 and MYOF. May also interact with EHD3 and EHD4.
Alternative Nomenclature	EH domain containing 2 antibody EH domain containing 3 antibody EHD 2 antibody EHD3 antibody FLJ96617 antibody PAST 2 antibody

General Information
Product	EHD3 Antibody
Description	Affinity Purified EH domain-containing protein 3 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse Rat
Immunogen	Synthetic peptide taken within amino acid region 475-535 on human EH-domain containing protein 3.
Accession #	NCBI: NP_055416.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.58 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:150
Immunopreciptiation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9NZN3
Overview	Plays a role in endocytic transport
Molecular Function	ATP binding;GTPase activity;nucleic acid binding;calcium ion binding;GTP binding
Subcellular Location	Cell membrane, Cytoplasm, Endosome, Membrane
Expression	Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.
Structure	Homooligomer, and heterooligomer with EHD1, EHD2 and EHD4
Alternative Nomenclature	EH domain containing 3 antibody EHD2 antibody EHD3 antibody PAST3 antibody

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Home page Primary Antibodies Signal Transduction Second Messengers cGMP C6orf150 Antibody

Home page Primary Antibodies Signal Transduction Second Messengers cGMP C6orf150 Antibody

Home page Primary Antibodies Signal Transduction G-Protein Coupled Receptors Chimaerin alpha 2 Antibody

Home page Primary Antibodies Signal Transduction G-Protein Coupled Receptors Chimaerin beta 2 Antibody

Home page Primary Antibodies Signal Transduction G-Protein Coupled Receptors Chimaerin beta 3 Antibody

Home page Primary Antibodies Epigenetics DNA / RNA EHD2 Antibody

Home page Primary Antibodies Signal Transduction Protein Transport Vesicle Transport EHD3 Antibody

ARF1 Antibody

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