General Information

Product

PDE12 Antibody

Description

Affinity Purified Phosphodiesterase 12 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 370-420 on rat PDE12 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q6TUH0

Overview

Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.

Molecular Function

Metal ion binding; poly (A)-specific ribonuclease activity

Subcellular Location

Mitochondrion Matrix

Expression

Ubiquitous

Structure

Belongs to the CCR4/nocturin family.

Alternative Nomenclature

2 PDE antibody

2' 5' phosphodiesterase 12 antibody

2' phosphodiesterase antibody

2'-PDE antibody

DKFZp667B1218 antibody

Pde12 antibody

Phosphodiesterase 12 antibody

General Information

Product

PDE12 Antibody

Description

Affinity Purified Phosphodiesterase 12 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 570-620 on human PDE12 protein.

Accession #

NCBI:  NP_001014020

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q6L8Q7

Overview

Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.

Molecular Function

Metal ion binding; poly (A)-specific ribonuclease activity

Subcellular Location

Mitochondrion Matrix

Expression

Ubiquitous

Structure

Belongs to the CCR4/nocturin family.

Alternative Nomenclature

2 PDE antibody

2' 5' phosphodiesterase 12 antibody

2' phosphodiesterase antibody

2',5'-phosphodiesterase 12 antibody

2'-PDE antibody

2-PDE antibody

2PDE antibody

DKFZp667B1218 antibody

Pde12 antibody

PDE12_HUMAN antibody

Phosphodiesterase 12 antibody

General Information

Product

AMPK alpha 1 Antibody

Description

Affinity Purified 5'-AMP-activated protein kinase catalytic subunit alpha-1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-550 on rat 5'-AMP-activated protein kinase catalytic subunit alpha-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P54645

Overview

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.

Molecular Function

[acetyl-CoA carboxylase] kinase activity

[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

AMP-activated protein kinase activity

ATP binding

chromatin binding

Subcellular Location

Nucleus, Cytoplasm

Expression

Low expression in kidney, liver, lung, heart and brain.

Structure

The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.

Alternative Nomenclature

5 AMP activated protein kinase alpha 1catalytic subunit antibody

AAPK1 antibody

ACACA kinase antibody

acetyl CoA carboxylase kinase antibody

AMP -activate kinase alpha 1 subunit antibody

AMPK 1 antibody

AMPK alpha 1 antibody

HMG CoA reductase kinase antibody

HMGCR kinase antibody

hormone sensitive lipase kinase antibody

Hydroxymethylglutaryl CoA reductase kinase antibody

im:7154392 antibody

kinase AMPK alpha1 antibody

PRKAA 1 antibody

Protein kinase AMP activated alpha 1 catalytic subunit antibody

SNF1A antibody

Tau protein kinase PRKAA1 antibody

General Information

Product

AMPK alpha 2 Antibody

Description

Affinity Purified 5'-AMP-activated protein kinase catalytic subunit alpha-2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 480-530 on rat 5'-AMP-activated protein kinase catalytic subunit alpha-2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q09137

Overview

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators.

Molecular Function

[acetyl-CoA carboxylase] kinase activity

[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

AMP-activated protein kinase activity

ATP binding Source: RGD

chromatin binding

histone serine kinase activity

metal ion binding -KW

protein binding

Subcellular Location

Nucleus, Cytoplasm

Expression

Skeletal muscle, lower levels in liver, heart and kidney.

Structure

The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.

Alternative Nomenclature

5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody

ACACA kinase antibody

Acetyl-CoA carboxylase kinase antibody

AMPK alpha 2 chain antibody

AMPK subunit alpha-2 antibody

AMPK2 antibody

AMPKa2 antibody

HMGCR kinase antibody

Hydroxymethylglutaryl-CoA reductase kinase antibody

PRKAA antibody

PRKAA2 antibody

Protein kinase AMP activated alpha 2 catalytic subunit antibody

General Information

Product

GBA Antibody

Description

Affinity Purified Glucosylceramidase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Recombinant Glucosylceramidase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P04062

Overview

Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:9201993, PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (PubMed:19279011). Also plays a role in cholesterol metabolism (PubMed:24211208, PubMed:26724485). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208, PubMed:26724485). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (PubMed:24211208). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (PubMed:24211208, PubMed:26724485).

Molecular Function

glucosylceramidase activity

glucosyltransferase activity

hydrolase activity

scavenger receptor binding

signaling receptor binding

steryl-beta-glucosidase activity

Subcellular Location

Lysosome

Expression

Interacts with saposin-C (PubMed:10781797). Interacts with SCARB2 (PubMed:18022370). Interacts with TCP1 (PubMed:21098288). May interact with SNCA; may inhibit the glucosylceramidase activity (PubMed:23266198). Interacts with GRN; this interaction prevents aggregation of GBA-SCARB2 complex via interaction with HSPA1A upon stress (PubMed:27789271).

Structure

Belongs to the glycosyl hydrolase 30 family.

Alternative Nomenclature

Lysosomal acid glucosylceramidase

General Information

Product

GAPDH Antibody

Description

Affinity Purified Glyceraldehyde-3-Phosphate dehydrogenase isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on mouse Glyceraldehyde-3-phosphate dehydrogenase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P16858

Overview

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively

Molecular Function

Oxidoreductase, Transferase

Subcellular Location

Cytoplasm, Cytoskeleton, Nucleus

Expression

brain;cerebullum;heart;kidney;liver;skeletal muscle

Structure

Homotetramer

Alternative Nomenclature

AI747587 antibody

cytoplasmic antibody

EC 1.1.1.8 antibody

FLJ26652 antibody

G3PD antibody

Gdc-1 antibody

Gdp1 antibody

Glycerol 3 phosphate dehydrogenase 1 antibody

Glycerol 3 phosphate dehydrogenase cytosolic antibody

Glycerol 3 phosphate dehydrogenase soluble antibody

Glycerol-3-phosphate dehydrogenase [NAD+] antibody

Glycerphosphate dehydrogenase antibody

GPD-C antibody

gpd1 antibody

Gpd3 antibody

GPDA_HUMAN antibody

Gpdc antibody

GPDH antibody

GPDH-C antibody

HTGTI antibody

KIAA4010 antibody

MGC93453 antibody

MGPD antibody

mKIAA4010 antibody

General Information

Product

GAPDH Antibody

Description

Affinity Purified Glyceraldehyde-3-Phosphate dehydrogenase isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human Glyceraldehyde-3-phosphate dehydrogenase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P04406

Overview

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively

Molecular Function

Oxidoreductase, Transferase

Subcellular Location

Cytoplasm, Cytoskeleton, Nucleus

Expression

brain;cerebullum;heart;kidney;liver;skeletal muscle

Structure

Homotetramer

Alternative Nomenclature

AI747587 antibody

cytoplasmic antibody

EC 1.1.1.8 antibody

FLJ26652 antibody

G3PD antibody

Gdc-1 antibody

Gdp1 antibody

Glycerol 3 phosphate dehydrogenase 1 antibody

Glycerol 3 phosphate dehydrogenase cytosolic antibody

Glycerol 3 phosphate dehydrogenase soluble antibody

Glycerol-3-phosphate dehydrogenase [NAD+] antibody

Glycerphosphate dehydrogenase antibody

GPD-C antibody

gpd1 antibody

Gpd3 antibody

GPDA_HUMAN antibody

Gpdc antibody

GPDH antibody

GPDH-C antibody

HTGTI antibody

KIAA4010 antibody

MGC93453 antibody

MGPD antibody

mKIAA4010 antibody

General Information

Product

SAPOSIN-C Antibody

Description

Affinity Purified Saposin-C Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 460-510 on mouse Saposin-C.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q3UFE8

Overview

beta-galactosidase activity

ganglioside GM1 binding

ganglioside GM2 binding

ganglioside GM3 binding

ganglioside GP1c binding

ganglioside GT1b binding

phospholipid binding

protease binding

protein homodimerization activity

Molecular Function

protease binding

protein homodimerization activity

Subcellular Location

Endosome

late endosome

Extracellular region or secreted

extracellular space

Lysosome

Alternative Nomenclature

A1 activator antibody
Cerebroside sulfate activator antibody
Co-beta-glucosidase antibody
Component C antibody
CSAct antibody
Dispersin antibody
GLBA antibody
Glucosylceramidase activator antibody
Proactivator polypeptide antibody
Proactivator polypeptide precursor antibody
Prosaposin (sphingolipid activator protein 1) antibody
prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody
Prosaposin antibody
Protein A antibody
Protein C antibody
PSAP antibody
SAP 1 antibody
SAP 2 antibody
SAP_HUMAN antibody
SAP1 antibody
Saposin A antibody
Saposin B antibody
Saposin B Val antibody
Saposin C antibody
Saposin D antibody
Saposins antibody
Sgp1 antibody
Sphingolipid activator protein 1 antibody
Sphingolipid activator protein 2 antibody
Sulfated glycoprotein 1 antibody
Sulfatide/GM1 activator antibody