General Information

Product

VPS29 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 133-183 on rat Vacuolar Protein Sorting-Associated Protein 29.

Accession #

NCBI:  NP_001099402.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-1.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9UBQ0

Overview

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3.

Molecular Function

Metal ion binding, Phosphoserine phosphatase activity

Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome Curated. Late endosome.

Expression

Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.

Structure

Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35 (PubMed:11102511, PubMed:16737443, PubMed:17891154). The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform. The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants.

Alternative Nomenclature

DC15 antibody

DC7 antibody

DKFZp564F0223 antibody

FLJ20492 antibody

hVPS29 antibody

PEP11 antibody

Retromer protein antibody

Vacuolar protein sorting 29 antibody

Vacuolar sorting protein VPS29/PEP11 antibody

Vesicle protein sorting 29 antibody

VPS 29 antibody

x 007 protein antibody

General Information

Product

TRIF Antibody 

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on TRIF protein.

Accession #

GenBank:  BAA76376.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q9CQE0

Overview

E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway (By similarity).

Molecular Function

Ligase, Zinc ion binding

Subcellular Location

Nucleus

Structure

Interacts with NLK (via C-terminus).

Alternative Nomenclature

MGC35334 antibody

Proline rich vinculin and TIR domain containing protein B antibody

Proline-rich antibody

PRVTIRB antibody

Putative NF kappa B activating protein 502H antibody

Putative NFkB activating protein antibody

TICAM 1 antibody

TIR domain-containing adapter protein inducing IFN-beta antibody

Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody

Toll like receptor adaptor molecule 1 antibody

Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody

TRIF antibodyvinculin and TIR domain-containing protein B antibody

General Information

Product

Syk Antibody

Description

Affinity Purified tyrosine-protein kinase SYK Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Syk protein.

Accession #

NCBI:  NP_001167639.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P43405

Overview

Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development.

Subcellular Location

Cell membrane, cytoplasm, cytosol

Expression

Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.

Alternative Nomenclature

EC 2.7.10.2 antibody

kinase Syk antibody

KSYK antibody

p72-Syk antibody

Spleen tyrosine kinase antibody

Syk antibody

Tyrosine protein kinase SYK antibody

General Information

Product

SYDE1 Antibody

Description

Affinity Purified Rho GTPase-activating protein SYDE1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

C. elegans, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 680-735 on human SYDE1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q6ZW31

Overview

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

Molecular Function

Rho GTPase activator activity

Subcellular Location

Cerebellar mossy fiber, synaptic membrane, cytosol

Alternative Nomenclature

7h3 antibody

Protein syd 1 homolog 1 antibody

Rho GTPase activating protein SYDE1 antibody

SYDE1 antibody

Synapse defective protein 1 homolog 1 antibody

General Information

Product

PDE1A7 Antibody Affinity Purified

Description

Affinity Purified Calcium/Calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A isoform PDE1A7 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide selective only for PDE1A7 gene.

Accession #

NCBI:  NP_001009978.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

Q9JLL9

Overview

A divalent metal cation

Molecular Function

Hydrolase, calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity, metal ion binding

Alternative Nomenclature

3' 5' cyclic nucleotide phosphodiesterase antibody

5''-cyclic nucleotide phosphodiesterase 1A antibody

61 kDa Cam PDE antibody

Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A antibody

Cam PDE 1A antibody

HCAM 1 antibody

HSPDE1A antibody

MGC26303 antibody

PDE 1A antibody

Phosphodiesterase 1A antibody

General Information

Product

PDE9A Antibody

Description

Affinity Purified High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform a Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic cyclic peptide common to all PDE9A variants.

Accession #

NCBI:  NP_002597.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:100

ELISA

1:100

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

O76083

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.

Molecular Function

Hydrolase, 3', 5' -cyclic-GMP phosphodiesterase activity

Subcellular Location

Isoform PDE9A1: Cell projection, Cytoplasm, Golgi Apparatus, Endoplasmic reticulum, Isoform PDE9A2: Cell projection, Cytoplasm, Isoform PDE9A3: Cytoplasm, Endoplasmic Reticulum, Isoform PDE9A17: Cytoplasm, Endoplasmic reticulum

Expression

Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.

Alternative Nomenclature

FLJ90181 antibody

HSPDE9A2 antibody

PDE 9A antibody

Pde9a antibody

Phosphodiesterase 9A antibody

phosphodiesterase PDE9A21 antibody

General Information

Product

TREX1 Antibody

Description

Affinity Purified Three-prime repair exonuclease 1 Antibody

Verified Applications

ELISA, IHC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 220-245 on human TREX1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9NSU2

Overview

Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements.

Molecular Function

Exonucleus, Hydrolase, Nuclease

Subcellular Location

Nucleus, Cytoplasm, Endoplasmic reticulum membrane; Peripheral membrane protein

Expression

Detected in thymus, spleen, liver, brain, heart, small intestine and colon.

Structure

Homodimer. Interacts (via proline-rich region) with TCERG1/CA150 (via the second WW domain). Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, directly interacts with SET; this interaction does not result in TREX1 inhibition.

Alternative Nomenclature

3' 5' exonuclease TREX1 antibody

3' repair exonuclease 1 antibody

AGS1 antibody

AGS5 antibody

CRV antibody

Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody

DKFZp434J0310 antibody

DNase III antibody

DRN3 antibody

HERNS antibody

Three prime repair exonuclease 1 antibody

TREX1 antibody

General Information

Product

Timeless Antibody

Description

Affinity Purified Protein timeless homolog isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide:

SGTPRVHRKKRFQIEDEDD

coresponding to amino acids 1178-1197 on Mouse Timeless protein.

Accession #

NCBI:  NP_035719.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.98-1.26 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9R1X4

Overview

Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock.

Molecular Function

Developmental protein, protein heterodimerization activity, protein homodimerization activity

Subcellular Location

Nucleus

Expression

Predominantly and robustly expressed in proliferative organs (spleen, thymus, intestine and testis) compared to those more differentiated such as kidney and liver (at protein level). Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis.

Structure

Homodimer or homomultimer. 

Alternative Nomenclature

Protein timeless homolog antibody

TIM antibody

timeless circadian clock 1 antibody

TIMELESS1 antibody

Tof1 homolog antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit fly, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 400-450 and 502-552 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1. Interacts with CITED2. Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit fly, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody