General Information

Product

PDE1A Antibody

Description

Affinity Purified Phosphodiesterase 1A Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 51-101 on human PDE1A protein. 

Accession #

NCBI:  NP001009978.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q9JLL9

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP.

Molecular Function

Hydrolase, calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity, metal ion binding

Subcellular Location

Cytosol

Expression

Several tissues, including brain, kidney, testes and heart.

Structure

Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

Alternative Nomenclature

3' 5' cyclic nucleotide phosphodiesterase antibody
61 kDa Cam PDE antibody

Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A antibody Calcium/calmodulin-dependent 3'' antibody
Calmodulin-dependent Phosphodiesterase antibody
Cam PDE 1A antibody

HSPDE1A antibody
MGC26303 antibody
PDE1A antibody

Phosphodiesterase 1A antibody
Phosphodiesterase 1A calmodulin dependent antibody

General Information

Product

CCR4-NOT Antibody

Description

Affinity Purified CCR (yeast CCR4/NOT complex component) homolog Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 50-100 on C. elegans CCR (yeast CCR4/NOT complex) protein.

Accession #

NCBI: NP_001023608.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q8I4B4

Overview

Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.

Molecular Function

Developmental protein, Repressor

Subcellular Location

Cytoplasm > P-body; Nucleus

Alternative Nomenclature

CCR4-NOT transcription complex antibody

LENG2 Antibody

Leukocyte receptor cluster member 2 antibody

NOT3 (negative regulator of trasncription, yeast) homolog antibody

General Information

Product

IQSEC3 Antibody

Description

Affinity Purified IQ motif and SEC7 domain-containing protein 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1100-1150 on human IQ motif and SEC7 domain-containing protein 3.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunohistochemistry

1:150

Western Blot

1:500

Protein

Uniprot #

Q9UPP2

Overview

Acts as a guanine nucleotide exchange factor (GEF) for ARF1.

Molecular Function

ARF guanyl-nucleotide exchange factor activity

Subcellular Location

Cytoplasm

Expression

Expressed specifically in the adult brain, predominantly in the cerebral cortex and the olfactory bulb, but not in the fetal brain. Expressed only in mature neurons, but not in undifferentiated neural stem precursor cells (NSPCs), nor in glioma cells.

Structure

Coiled coil

Alternative Nomenclature

IQ motif and Sec7 domain 3 antibody

IQ motif and SEC7 domain-containing protein 3 antibody

Iqsec3 antibody

KIAA1110 antibody

MGC30156 antibody

General Information

Product

SQSTM1 / p62 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-440 on human Sequestosome-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13501

Overview

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

Molecular Function

identical protein binding, protein kinase binding, protein kinase C binding, protein serine/threonine kinase activity, receptor tyrosine kinase binding, SH2 domain binding, ubiquitin binding, ubiquitin protein ligase binding, zinc ion binding

Subcellular Location

Cytoplasm

Late endosome

Lysosome

Cytoplasmic vesicle › autophagosome

Nucleus

Endoplasmic reticulum

Cytoplasm › P-body

Expression

Ubiquitously expressed.

Structure

Homooligomer or heterooligomer; may form homotypic arrays. Dimerization interferes with ubiquitin binding.

Alternative Nomenclature

A170 

EBI 3 associated protein of 60 kDa 

EBIAP 

FTDALS3 

ORCA 

OSF-6 

Osi 

OSIL 

Oxidative stress induced like 

p60 

p62 

p62B 

Paget disease of bone 3 

PDB3 

Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa 

PKC-zeta-interacting protein 

Protein kinase C-zeta-interacting protein 

Sequestosome 1 

SQSTM 1 

STAP 

STONE14 

Ubiquitin-binding protein p62 

ZIP 

ZIP3 

General Information

Product

FGD3 Antibody

Description

Affinity Purified Faciogenital dysplasia 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-320 on human FYVE, RhoGEF and PH domain-containing protein 3.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q5JSP0

Overview

Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.

Molecular Function

guanyl-nucleotide exchange factor activity

metal ion binding

Rho guanyl-nucleotide exchange factor activity

small GTPase binding

Subcellular Location

Cytoplasm

Structure

Repeat, Zinc-finger

Alternative Nomenclature

Faciogenital dysplasia 3 antibody

FGD1 family, member 3 antibody

FGD3 antibody

FYVE antibody

FYVE, RhoGEF and PH domain containing 3 antibody

ZFYVE5 antibody

Zinc finger FYVE domain containing protein 5 antibody

General Information

Product

LIMK1 Antibody

Description

Affinity Purified LIM domain kinase 1 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.

Accession #

NCBI:  NP_002305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53667

Overview

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.

Molecular Function

ATP binding, protein kinase activity, protein serine/threonine kinase activity, zinc ion binding, heat shock protein binding

Subcellular Location

Cytoplasm, nucleus

Expression

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Alternative Nomenclature

EC 2.7.11.1 antibody

LIM domain kinase 1 antibody

LIM motif-containing protein kinase antibody

LIMK antibody

LIMK-1 antibody

General Information

Product

VPS36 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 250-310 on human Vacuolar Protein-Sorting-associated protein 36 protein.

Accession #

NCBI:  NP_057159

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q86VN1

Overview

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.

Molecular Function

phosphatidylinositol-3-phosphate binding

protein C-terminus binding

ubiquitin binding

Subcellular Location

Nucleus

Endosome

Late endosome

Expression

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45. Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (PubMed:14519844). Interacts with VPS25, SNF8, TSG101 and CHMP6 (PubMed:14505570, PubMed:14519844, PubMed:16973552, PubMed:18539118). Interacts (via GLUE domain) with ubiquitin (PubMed:17057716). Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (PubMed:17010938). Interacts with ECPAS (PubMed:20682791).

Structure

The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.

Alternative Nomenclature

C13orf9 antibody

CGI 145 antibody

EAP45 antibody

ELL associated protein of 45 kDa antibody

ESCRT-II complex subunit VPS36 antibody

Vacuolar protein-sorting-associated protein 36 antibody

Vps36 antibody

General Information

Product

GPS2 Antibody

Description

Affinity Purified G protein pathway suppressor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human G protein pathway suppressor 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q13227

Molecular Function

cyclin binding

GTPase inhibitor activity

transcription coactivator activity

transcription corepressor activity

Subcellular Location

Nucleus, Cytosol

Expression

Widely expressed.

Structure

Coiled coil

Alternative Nomenclature

AMF 1 antibody

G protein pathway suppressor 2 antibody

GPS 2 antibody

General Information

Product

PICK1 Antibody

Description

Affinity Purified PRKCA-binding protein / PICK1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-380 on human PRKCA-binding protein / PICK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9NRD5

Subcellular Location

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Expression

Ubiquitous.

Alternative Nomenclature

PICK 1 antibody
PRKCA-binding protein antibody
PRKCABP antibody
Protein interacting with C kinase 1 antibody
Protein interacting with PRKCA antibody
Protein interacting with PRKCA 1 antibody
Protein kinase C-alpha-binding protein antibody
Protein that interacts with C kinase 1 antibody