MLKL Antibody

Product no.: MLKL-101AP

$285.00

Description

General Information
Product	MLKL Antibody
Description	Affinity Purified Mixed lineage kinase domain-like protein MLKL Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to amino acids within region 400-450 on mouse Mixed lineage kinase domain-like protein MLKL.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5-1.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Purification Method	Immobilized antigen affinity chromatography
Clonality	Polyclonal
Storage	-20?C for long term storage
Application Protocol
ELISA	1:50,000
Immunoprecipitation	1:200
Western Blot	1:500-1:5,000
Protein
Uniprot #	Q9D2Y4
Overview	Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).
Molecular Function	ATP binding identical protein binding JUN kinase kinase kinase activity protein-containing complex binding protein kinase activity protein kinase binding
Subcellular Location	Nucleus, Plasma membrane, cytoplasm
Structure	Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414). Within this complex, may play a role in the proper targeti
Alternative Nomenclature	9130019I15Rik antibody FLJ34389 antibody hMLKL antibody Mixed lineage kinase domain like antibody Mixed lineage kinase domain like pseudokinase antibody Mixed lineage kinase domain-like protein antibody Mlkl antibody MLKL_HUMAN antibody

Protein information supplied by UniProt.