General Information

Product

CXCR7 Antibody

Description

Affinity Purified Chemokine (C-X-C motif) receptor 7 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human CXCR7 protein.

Accession #

GenBank: AAH36661

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P25106

Overview

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival.

Molecular Function

Cell adhesion, Host-virus interaction

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cytoplasm › perinuclear region. Early endosome. Recycling endosome

Expression

Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue.

Structure

Homodimer. Can form heterodimers with CXCR4; heterodimerization may regulate CXCR4 signaling activity. Interacts with ARRB1 and ARRB2.

Alternative Nomenclature

ACKR3 antibody

atypical chemokine receptor 3 antibody

C X C chemokine receptor type 7 antibody

Chemokine orphan receptor 1 antibody

CMKOR1 antibody

CXC R7 antibody

G protein coupled receptor 159 antibody

G protein coupled receptor RDC1 homolog antibody

GPR159 antibody

GPRN1 antibody

RDC 1 antibody

General Information

Product

Dopamine Receptor D3 Antibody

Description

Affinity Purified D(3) dopamine receptor Antibody

Verified Applications

Dot Blot, ELISA, ICC/IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Dopamine Receptor D3 protein, peptide was covalently modified.

Sequence: CQACHVSPELYRATTWGY

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:50,000

ELISA

1:50,000

Immunoprecipitation

Use at an assay dependent dilution. 2 µl of this antibody will immunoprecipitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells.

Western Blot

1:1,000-1:1,5000. Detects a band of approximately 50 kDa (predicted molecular weight 50 kDa).

Protein

Uniprot #

P19020

Overview

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation (By similarity).

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane, Multi-pass membrane protein

Expression

Mainly in limbic areas of brain.

Structure

Interacts with GRK4 and PALM (By similarity). Interacts with CLIC6.

Alternative Nomenclature

3dopamine receptor antibody

D antibody

D(3) dopamine receptor antibody

DRD3 antibody

ETM1 antibody

FET1 antibody

General Information

Product

Dopamine D4 Receptor Antibody Affinity Purified

Description

Affinity Purified Dopamine D4 receptor Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique sequence on Dopamine Receptor D4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:50,000

ELISA

1:50,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:1,000-1:1,5000

Protein

Uniprot #

P51436

Overview

Dopamine receptor mediated by G proteins which inhibit adenylyl cyclase.  Modulates circadian rhtym of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells.

Molecular Function

Dopamine binding; Biological rhythms

Subcellular Location

Cell membrane; Multi-pass membrane

Structure

Forms homo- and heterooligomers with DRD2.

Alternative Nomenclature

D(2C) dopamine receptor antibody

D(4) dopamine receptor (D(2C) dopamine receptor). antibody

DRD4 antibody

General Information

Product

PTGER3 Antibody

Description

Protaglandin Receptor EP3D Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on EP3 Receptor protein.

Accession #

NCBI:  XP_009209142.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.6 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

UPI0004F2137B

Overview

Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium. The various isoforms have identical ligand binding properties but can interact with different second messenger systems (By similarity).

Molecular Function

Prostaglandin E receptor activity, G-protein coupled receptor

Subcellular Location

Cell membrane; Multi-pass membrane

Expression

Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels.

Structure

Belongs to the G-protein coupled receptor 1 family.

Alternative Nomenclature

EP 3 antibody

EP3 I antibody

EP3 II antibody

EP3 III antibody

EP3 IV antibody

Ep3 prostanoid receptor antibody

Ep3 receptor antibody

EP3 subtype antibody

EP3e antibody

MGC141828 antibody

MGC141829 antibody

PGE receptor EP3 subtype antibody

PGE2 R antibody

Pgerep3 antibody

Prostaglandin E receptor EP3 subtype 3 isoform antibody

Prostanoid EP3 receptor antibody

PTGER 3 antibody

Rep3 antibody

rEP3a antibody

rEP3b antibody

General Information

Product

GPCR GPR40 Antibody

Description

Affinity Purified FFAR1 protein Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human FFA1 protein.

Accession #

GenBank: AAI20944.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q0IJ71

Molecular Function

G-protein coupled receptor activity

Subcellular Location

Integral component of membrane

Alternative Nomenclature

FFA1R antibody

Free fatty acid receptor 1 antibody

G protein coupled receptor 40 antibody

GPR40 antibody

General Information

Product

GIRK3 Antibody Affinity Purified

Description

Affinity Purified G protein-activated inward rectifier potassium channel 3 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid region on human GIRK3 protein.

Accession #

NCBI:  NP_004974.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q92806

Molecular Function

Potassium

Subcellular Location

Membrane; Multi-pass membrane protein

Structure

Belongs to inward rectifier-type potassium channel family

Alternative Nomenclature

G protein activated inward rectifier potassium channel 3 antibody

GIRK3 antibody

Inward rectifier K(+) channel Kir3.3 antibody

inwardly rectifying subfamily J member 9 antibody

Kcnj9 antibody

KIR3.3 antibody

Potassium channel antibody

Potassium channel inwardly rectifying subfamily J member 9 antibody

General Information

Product

GLP2R Antibody

Description

Affinity Purified Glucagon like peptide 2 Receptor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 375-425 on human GLP2R protein.

Accession #

GenBank: AAH43390.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q86XI5

Overview

GPCR activity

Subcellular Location

Cell membrane; Membrane

Alternative Nomenclature

GLP 2 R antibody

GLP 2 receptor antibody

GLP2R antibody

Glucagon-like peptide 2 receptor antibody

General Information

Product

Glutamate Receptor 2 Antibody

Description

Affinity Purified Glutamate receptor GLUR2 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Pig

Immunogen

Synthetic peptide corresponding to unique amino acid region on GluR2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50

Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:500

Protein

Uniprot #

P42262

Overview

L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.

Molecular Function

Ion Transport

Subcellular Location

Cell membrane; Multi-pass membrane protein; Dendrite

Expression

Brain

Structure

Homotetramer or heterotetramer of pore forming glutamate receptor subunits.  Tetramers may be formed by the dimerization of dimers.

Alternative Nomenclature

AMPA 2 antibody

AMPA selective glutamate receptor 2 antibody

GluA2 antibody

GluR-K2 antibody

GLUR2 antibody

GLURB antibody

Glutamate receptor 2 antibody

Glutamate receptor ionotropic AMPA 2 antibody

Glutamate receptor ionotropic antibody

Gria2 antibody

HBGR2 antibody

General Information

Product

GRIK1 Antibody Affinity Purified

Description

Affinity Purified Glutamate receptor GLUR5 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid region on GluR5.

Accession #

NCBI: NP_666184

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8BRT2

Overview

Extracellular-glutamate-gated ion channel activity

Molecular Function

Ion transport

Subcellular Location

Cell junction, Cell membrane, Synapse

Structure

Transmembrane helix

Alternative Nomenclature

Glutamate receptor metabotropic 5 antibody

GPRC1E antibody

GRM5 antibody

Metabotropic glutamate receptor 5 variant F antibody

Metabotropic glutamate receptor 5 variant G antibody

Metabotropic glutamate receptor 5 variant H antibody

mGlu5 antibody

mGluR5a antibody

mGluR5b antibody

General Information

Product

Glutamate Receptor 1 Antibody Affinity Purified

Description

Affinity Purified Glutamate receptor GLUR1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid reigon 850-950.

Accession #

GeneBank: CAA41491

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P42261

Overview

L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.

Molecular Function

Ion Transport

Subcellular Location

Cell membrane; Multi-pass membrane protein; Dendrite

Expression

Brain

Structure

Homotetramer or heterotetramer of pore forming glutamate receptor subunits.  Tetramers may be formed by the dimerization of dimers.

Alternative Nomenclature

AMPA 1 antibody

AMPA selective glutamate receptor 1 antibody

GluA1 antibody

GLUH 1 antibody

GluR-1 antibody

GluR-A antibody

GluR-K1 antibody

Glutamate receptor 1 antibody

Glutamate receptor ionotropic AMPA 1 antibody

HBGR1 antibody

MGC133252 antibody

OTTHUMP00000160643 antibody

OTTHUMP00000165781 antibody

OTTHUMP00000224241 antibody

OTTHUMP00000224242 antibody

OTTHUMP00000224243 antibody