General Information
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Product
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SOCS1 Antibody FITC-Conjugated
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Description |
FITC-Conjugated Suppressor of Cytokine Signaling 1 Antibody |
Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 170-212 on human Suppressor of Cytokine signaling 1 protein.
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Accession #
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GenBank: CAA27974.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.64-0.72 µg/µl in antibody stabilization buffer
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Form
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FITC-Conjugated
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunohistochemistry |
1:100 |
Immunoprecipitation |
1:200 |
Western Blot
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1:500
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Protein
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Uniprot #
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P13848
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Overview
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SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.
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Molecular Function
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Insulin-like growth factor receptor binding; Protein kinase binding; Kinase inhibitor activity
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Subcellular Location
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Nucleus; Cytoplasmic vesicle
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Expression
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Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
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Structure
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Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC (SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR.
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Alternative Nomenclature
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CISH 1 antibody
Cytokine Inducible SH2 Protein 1 antibody
JAB antibody
JAK-binding protein antibody
Janus kinase binding protein antibody
SOCS-1 antibody
SSI1 antibody
STAT Induced STAT Inhibitor 1 antibody
Suppressor of cytokine signaling 1 antibody
TEC Interacting Protein 3 antibody
TIP3 antibody
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