General Information |
|
Product |
Phospho PKC eta (T513) Antibody FITC-Conjugated |
Description |
FITC-Conjugated Phosphorylated PKC eta T513 Antibody C-epitope |
Verified Applications |
ELISA, IMM, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Mouse, Rat |
Immunogen |
Synthetic peptide taken within amino acid region 500-550 on human PKC eta protein. Phosphorylated T513. |
Accession # |
NCBI: NP_006246 |
|
|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.7 µg/µl in antibody stabilization buffer |
Form |
FITC-Conjugated |
Clonality |
Polyclonal |
Determinant |
C-epitope |
Storage |
-20⁰C for long term storage |
|
|
DB |
1:10,000 |
ELISA |
1:10,000 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500 |
|
|
Uniprot # |
|
Overview |
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. Is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization. |
Molecular Function |
Kinase; Serine/threonine-protein kinase, Transferase |
Subcellular Location |
Cytoplasm |
Expression |
Most abundant in lung, less in heart and skin. |
Structure |
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. |
Alternative Nomenclature |
MGC 5363 antibody MGC26269 antibody MGC5363 antibody PKC L antibody PRKCH antibody PRKCL antibody Protein kinase C eta type antibody |
Protein information supplied by UniProt
Product | Note | Status | Price | |
---|---|---|---|---|
|
PPKCETA-240AP | |||
|
P-PPKCETA.240 | |||
|
PPKCETA.240-BIOTIN | |||
|
PC-PPKCETA | |||
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