General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 130-180 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

Phospho Src Antibody

Description

Affinity Purified Phosphorylated Proto-oncogene tyrosine-protein kinase Src Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope phosphorylated synthetic peptide taken within amino acid region 400-500 on human Src protein.  Phosphorylated Tyr-416 residue.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

Neuronal CSRC tyrosine specific protein kinase antibody

p60 Src antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

Phospho-Src Antibody

Description

Affinity Purified Phosphorylated Proto-oncogene tyrosine-protein kinase Src Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope phosphorylated synthetic peptide taken within amino acid region 200-300 on human Src protein.  Phosphorylated Tyr-215 residue.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

Neuronal CSRC tyrosine specific protein kinase antibody

p60 Src antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

Src Antibody

Description

Proto-oncogene tyrosine-protein kinase Src Antibody C-epitope

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-500 on human Src protein.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.60 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation.

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

c SRC antibody

CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody

EC 2.7.10.2 antibody

Neuronal CSRC tyrosine specific protein kinase antibody

Neuronal SRC antibody

Oncogene SRC antibody

p60 Src antibody

Proto-oncogene tyrosine-protein kinase Src antibody

SRC proto oncogene non receptor tyrosine kinase antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

Tyrosine protein kinase SRC1 antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

Src Antibody

Description

Affinity Purified Proto-oncogene tyrosine-protein kinase Src Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-300 on human Src protein.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.60 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

c SRC antibody

CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody

EC 2.7.10.2 antibody

Neuronal CSRC tyrosine specific protein kinase antibody

Neuronal SRC antibody

Oncogene SRC antibody

p60 Src antibody

Proto oncogene tyrosine protein kinase Src antibody

SRC Oncogene antibody

SRC proto oncogene non receptor tyrosine kinase antibody

SRC1 antibody

vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

General Information

Product

FAK1 Antibody

Description

Affinity Purified Focal Adhesion Kinase 1

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 740-790 on human focal adhesion kinase 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q05397

Overview

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Molecular Function

Developmental protein; Kinase; Transferase; Tyrosine-protein kinase

Subcellular Location

Cell junction › focal adhesion

Cell membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasm › cell cortex

Cytoplasm › cytoskeleton

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Nucleus

Expression

Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Contains 1 FERM domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

FADK 1 antibody

FADK antibody

FAK 1 antibody

FAK related non kinase polypeptide antibody

FAK1 antibody

Focal adhesion kinase 1 antibody

Focal adhesion kinase isoform FAK Del33 antibody

Focal adhesion kinase related nonkinase antibody

FRNK antibody

p125FAK antibody

pp125FAK antibody

PPP1R71 antibody

Protein phosphatase 1 regulatory subunit 71 antibody

Protein tyrosine kinase 2 antibody

Protein Tyrosine Kinase Cytoplasmic antibody

PTK 2 antibody

PTK2 protein tyrosine kinase 2 antibody

General Information

Product

FAK2 Antibody

Description

Affinity Purified Protein-tyrosine kinase 2-beta / Focal adhesion Kinase 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human focal adhesion kinase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q14289

Overview

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

Molecular Function

Kinase, Transferase, Tyrosine-protein Kinase

Subcellular Location

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Expression

Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Contains 1 FERM domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

CADTK antibody

CAK beta antibody

CAKB antibody

Calcium dependent tyrosine kinase antibody

Calcium regulated non receptor proline rich tyrosine kinase antibody

Cell adhesion kinase beta antibody

E430023O05Rik antibody

EC 2.7.10.2 antibody

FADK2 antibody

FAK2 antibody

Focal Adhesion Kinase 2 antibody

MGC124628 antibody

PKB antibody

pp125FAK antibody

Proline Rich Tyrosine Kinase 2 antibody

Protein kinase B antibody

Protein Tyrosine Kinase 2 Beta antibody

PTK antibody

PTK2B protein tyrosine kinase 2 beta antibody

PYK 2 antibody

RAFTK antibody

RAFTK2 antibody

Related adhesion focal tyrosine kinase antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 and 950-976 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

Prolactin Antibody

Description

Affinity Purified Prolactin Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IHC, IF, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human Prolactin protein.

Accession #

GenBank:  CAA38264.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

Dot Blot

1:50,000

ELISA

1:50,000

Immunocytochemistry

1:100

Immunohistochemistry

1:100

Western Blot

1:500

Protein

Uniprot #

P01236

Overview

Prolactin acts primarily on the mammary gland by promoting lactation.

Molecular Function

prolactin receptor binding, hormone

Subcellular Location

Secreted

Alternative Nomenclature

Decidual prolactin antibody

Lactogenic Hormone antibody

Luteotropic Hormone antibody

Mammotropin antibody

PRL antibody