General Information

Product

Disembodied Antibody Affinity Purified

Description

Affinity Purified Cytochrome P450 302a1, mitochondrial Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique sequence on Disemboided protein.

Accession #

NCBI: AAF60174.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:500

Protein

Uniprot #

Q9NGX9

Overview

Negatively regulates glial cell division in the embryonic midline.  Invovled in metabolism of insect hormones.

Molecular Function

Steroid biosynthesis; ecdysteroid biosynthesis

Subcellular Location

Mitochondrion membrane

Expression

Complex coexpression of disembodied and shade in early embryo that restricts to the prothracic gland cells of developing ring gland during late embryogenesis.

Structure

Belongs to cytochrome P450 family

Alternative Nomenclature

dib Antibody

General Information

Product

Disembodied Antibody Affinity Purified

Description

Affinity Purified Cytochrome P450 302a1, mitochondrial Antibody

Verified Applications

CM, ELISA, ICC, IF, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique sequence on Disemboided protein.

Accession #

NCBI: AAF60174.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:500

Protein

Uniprot #

Q9NGX9

Overview

Negatively regulates glial cell division in the embryonic midline.  Invovled in metabolism of insect hormones.

Molecular Function

Steroid biosynthesis; ecdysteroid biosynthesis

Subcellular Location

Mitochondrion membrane

Expression

Complex coexpression of disembodied and shade in early embryo that restricts to the prothracic gland cells of developing ring gland during late embryogenesis.

Structure

Belongs to cytochrome P450 family

Alternative Nomenclature

dib Antibody

General Information

Product

Dfos Antibody

Description

Affinity Purified Drosophila dFRA mRNA for Fos-related antigen Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 450-550 on DFos protein.

Accession #

GenBank: X54143.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P21525

Overview

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'/  May play a role in the function or determination of a particular subset of cells in the developing embryo.

Molecular Function

DNA-Binding

Subcellular Location

Nucleus

Expression

Early expression in mesodermal and cephalic furrow.  Late embryonic development expression is ectodermal, corresponding to muscle attachment sites.  Also in midgut, hindgut and analpad.

Structure

Homodimer; Heterodimer with Jra

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

G0 G1 switch regulatory protein 7 antibody

G0S7 antibody

Oncogene FOS antibody

p55 antibody

proto oncogene c Fos antibody

v fos FBJ murine osteosarcoma viral oncogene homolog antibody

General Information

Product

Dfos Antibody

Description

Affinity Purified Drosophila dFRA mRNA for Fos-related antigen Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 5-80 on DFos protein.

Accession #

GenBank: X54143.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P21525

Overview

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'/  May play a role in the function or determination of a particular subset of cells in the developing embryo.

Molecular Function

DNA-Binding

Subcellular Location

Nucleus

Expression

Early expression in mesodermal and cephalic furrow.  Late embryonic development expression is ectodermal, corresponding to muscle attachment sites.  Also in midgut, hindgut and analpad.

Structure

Homodimer; Heterodimer with Jra

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

G0 G1 switch regulatory protein 7 antibody

G0S7 antibody

Oncogene FOS antibody

p55 antibody

proto oncogene c Fos antibody

v fos FBJ murine osteosarcoma viral oncogene homolog antibody

General Information

Product

JunD Antibody Affinity Purified N-epitope

Description

Affinity Purified Djun Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique sequence on Drosophila melangoaster Jun protein.

Accession #

GenBank:  AAA28650

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:500

Protein

Uniprot #

P18289

Overview

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Molecular Function

Activator; Transcription regulation;

Subcellular Location

Nucleus

Expression

Expressed during embryonic development

Structure

AP-1/dJRA interacts with AP-1/dFra to form a dimer. The dFra-dJRA complex is bound more stably to the AP-1 site than either of the two proteins alone.

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

Transcription factor jun D antibody

General Information

Product

JunD Antibody Affinity Purified

Description

Affinity Purified Djun Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique sequence on Drosophila melangoaster Jun protein.

Accession #

Gen Bank: AAA28650

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:500

Protein

Uniprot #

P18289

Overview

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Molecular Function

Activator; Transcription regulation;

Subcellular Location

Nucleus

Expression

Expressed during embryonic development

Structure

AP-1/dJRA interacts with AP-1/dFra to form a dimer. The dFra-dJRA complex is bound more stably to the AP-1 site than either of the two proteins alone.

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

Transcription factor jun D antibody

General Information

Product

Saxophone Antibody

Description

Affinity Purified Serine/Threonine kinase receptor SAX (sax) Antibody N-epitope

Verified Applications

ELISA, ICC, IHC-FoFr, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Synthetic peptide corresponding to amino acids 107-125 of D. melanogaster SAX protein. Peptide was conjugated to KLH.

Sequence:

GCSTSPDQLPMICSQNSLK

Accession #

GenBank: U11441.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q7JPM7

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase

Alternative Nomenclature

CG1891 antibody

receptor protein serine/threonine kinase antibody

saxophone antibody

X linked surface antigen antibody

General Information

Product

Saxophone Antibody Affinity Purified

Description

Affinity Purified Serine/Threonine kinase receptor SAX (sax) mRNA, complete cds Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequence near C-terminal of Saxophone protein.

Accession #

GenBank: U11441.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q7JPM7

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase

Alternative Nomenclature

CG1891 antibody

receptor protein serine/threonine kinase antibody

saxophone antibody

X linked surface antigen antibody

General Information

Product

Bestrophin 4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 400-500 on human Bestrophin 4 protein.

Accession #

NCBI:  NP_695006.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.20 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q8NFU0

Overview

Forms calcium-sensitive chloride channels. Permeable to bicarbonate.

Molecular Function

Chloride Channel

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Predominantly found in colon and the weakly in fetal brain, spinal cord, retina, lung, trachea, testis and placenta.

Structure

Belongs to bestrophin Family

Alternative Nomenclature

BEST 4 antibody

MGC126872 antibody

Vitelliform macular dystrophy 2 like 2 antibody

VMD2-like gene 2 antibody

VMD2L2 antibody

General Information

Product

eIF4E3 Antibody Affinity Purified

Description

Affinity Purified Eukaryotic translation initiation factor 4E-3 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 150-240 on eIF4E3 protein.

Accession #

GenBank:  AAF50460.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9VSG1

Overview

translation initiation factor activity

Molecular Function

Initiation factor

Subcellular Location

cytoplasm

Alternative Nomenclature

eIF-4E type 3 antibody

Eukaryotic translation initiation factor 4E type 3 antibody