General Information

Product

Olig2 Antibody

Description

Affinity Purified OLIG2 protein Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to Human Olig2 amino acids 136-156. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to a carrier protein using hetero-bifunctional cross linker for immunogen preparation.

Sequence:

PYAHGPSVRKLSKIATLLLAR

Accession #

GenBank:  AAH36245.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13516

Overview

Necessary for oligodendrocyte and motor neuron specification in the spinal cord.  In the hindbrain it is  needed in the development of somatic neurons.

Molecular Function

Developmental protein, DNA binding

Subcellular Location

Nucleus; Cytoplasm

Expression

Is expressed in the brain in oligodendrocytes.  Is expressed strongly in oligodendrogliomas with weaker expression in astrocytomas

Structure

Interacts with NKX2-3

Alternative Nomenclature

Basic domain helix loop helix protein class B 1 antibody

BHLHB antibody

bHLHB1 antibody

bHLHe19 antibody

Class B basic helix loop helix protein 1 antibody

class E basic helix loop helix protein 19 antibody

Human protein kinase C binding protein RACK17 antibody

Olig2 antibody

Oligo2 antibody

Oligodendrocyte lineage transcription factor 2 antibody

Oligodendrocyte specific bHLH transcription factor 2 antibody

PRKCBP2 antibody

Protein kinase C binding protein 2 antibody

Protein kinase C binding protein RACK17 antibody

RACK17 antibody

General Information

Product

Dfos Antibody

Description

Affinity Purified Drosophila dFRA mRNA for Fos-related antigen Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 5-80 on DFos protein.

Accession #

GenBank: X54143.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P21525

Overview

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'/  May play a role in the function or determination of a particular subset of cells in the developing embryo.

Molecular Function

DNA-Binding

Subcellular Location

Nucleus

Expression

Early expression in mesodermal and cephalic furrow.  Late embryonic development expression is ectodermal, corresponding to muscle attachment sites.  Also in midgut, hindgut and analpad.

Structure

Homodimer; Heterodimer with Jra

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

G0 G1 switch regulatory protein 7 antibody

G0S7 antibody

Oncogene FOS antibody

p55 antibody

proto oncogene c Fos antibody

v fos FBJ murine osteosarcoma viral oncogene homolog antibody

General Information

Product

Dfos Antibody

Description

Affinity Purified Drosophila dFRA mRNA for Fos-related antigen Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 450-550 on DFos protein.

Accession #

GenBank: X54143.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P21525

Overview

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'/  May play a role in the function or determination of a particular subset of cells in the developing embryo.

Molecular Function

DNA-Binding

Subcellular Location

Nucleus

Expression

Early expression in mesodermal and cephalic furrow.  Late embryonic development expression is ectodermal, corresponding to muscle attachment sites.  Also in midgut, hindgut and analpad.

Structure

Homodimer; Heterodimer with Jra

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

G0 G1 switch regulatory protein 7 antibody

G0S7 antibody

Oncogene FOS antibody

p55 antibody

proto oncogene c Fos antibody

v fos FBJ murine osteosarcoma viral oncogene homolog antibody

General Information

Product

Ki67 Antibody

Description

Affinity Purified Antigen KI-67 isoform 1 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 2950-3000 on human antigen Ki67 isoform 1.

Accession #

NCBI:  NP_002408.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P46013

Overview

required for maintaining cell proliferation

Molecular Function

poly(A) RNA binding;protein C-terminus binding;ATP binding

Subcellular Location

Chromosome, Nucleus

Expression

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Alternative Nomenclature

Antigen identified by monoclonal antibody Ki 67 antibody

KIA antibody

MKI67 antibody

Proliferation related Ki 67 antigen antibody

RP11-380J17.2 antibody

General Information

Product

Ki67 Antibody

Description

Affinity Purified Antigen KI-67 isoform 1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1250-1300 on human antigen Ki67 isoform 1.

Accession #

NCBI:  NP_002408.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P46013

Overview

required for maintaining cell proliferation

Molecular Function

poly(A) RNA binding; protein C-terminus binding; ATP binding

Subcellular Location

Chromosome, Nucleus

Expression

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Alternative Nomenclature

Antigen identified by monoclonal antibody Ki 67 antibody

KIA antibody

MKI67 antibody

Proliferation related Ki 67 antigen antibody

RP11-380J17.2 antibody

General Information

Product

SOX10 Antibody

Description

Affinity Purified Transcription Factor SOX-10 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human Transcription Factor SOX-10 protein.

Accession #

NCBI:  NP_008872.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P56693

Overview

Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.

Molecular Function

Chromatin binding; Identical protein binding; RNA polymerase II transcription factor binding transcription factor activity invovled in positive regulation of transcription.

Subcellular Location

Cytoplasm; Nucleus

Expression

Expressed in fetal brain and in adult brain, heart, small intestine and colon.

Structure

Contains 1 HMG box DNA-binding domain.

Alternative Nomenclature

DOM 

MGC15649 

PCWH 

SOX 10 

SRY (sex determining region Y) box 10 

SRY related HMG box gene 10 

Transcription factor SOX 10 

WS2E 

WS4 

WS4C 

General Information

Product

SOX10 Antibody

Description

Affinity Purified Transcription Factor SOX-10 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 10-60 on human Transcription Factor SOX-10 protein.

Accession #

NCBI:  NP_008872.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P56693

Overview

Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.

Molecular Function

Chromatin binding; Identical protein binding; RNA polymerase II transcription factor binding transcription factor activity invovled in positive regulation of transcription.

Subcellular Location

Cytoplasm; Nucleus

Expression

Expressed in fetal brain and in adult brain, heart, small intestine and colon.

Structure

Contains 1 HMG box DNA-binding domain.

Alternative Nomenclature

DOM antibody

Dominant megacolon mouse human homolog of antibody

MGC15649 antibody

PCWH antibody

SOX 10 antibody

SRY (sex determining region Y) box 10 antibody

SRY box 10 antibody

SRY box containing gene 10 antibody

SRY related HMG box gene 10 antibody

Transcription factor SOX 10 antibody

WS2E antibody

WS4 antibody

WS4C antibody

General Information

Product

SOX2 Antibody

Description

Affinity Purified Sex determing region Y-box 2 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human Sex determining region Y-box 2 protein.

Accession #

GenBank: AJF45879.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

A0A0B5HSU3

Overview

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

Molecular Function

Chromatin binding; DNA binding; miRNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Subcellular Location

Nucleus

Structure

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.

Alternative Nomenclature

ANOP3 antibody

cb236 antibody

Delta EF2a antibody

lcc antibody

MCOPS3 antibody

MGC148683 antibody

MGC2413 antibody

RGD1565646 antibody

SRY (sex determining region Y) box 2 antibody

SRY box containing gene 2 antibody

SRY related HMG box gene 2 antibody

SRY-box 2 antibody

Transcription factor SOX 2 antibody

ysb antibody

General Information

Product

SOX2 Antibody

Description

Affinity Purified Sex determing region Y-box 2 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human Sex determing region Y-box 2 protein.

Accession #

GenBank: AJF45879.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

A0A0B5HSU3

Overview

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation.

Molecular Function

Chromatin binding; DNA binding; miRNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription.

Subcellular Location

Nucleus

Structure

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.

Alternative Nomenclature

ANOP3 antibody

cb236 antibody

Delta EF2a antibody

lcc antibody

MCOPS3 antibody

MGC148683 antibody

MGC2413 antibody

RGD1565646 antibody

SRY (sex determining region Y) box 2 antibody

SRY box containing gene 2 antibody

SRY related HMG box gene 2 antibody

SRY-box 2 antibody

Transcription factor SOX 2 antibody

ysb antibody

General Information

Product

Synaptophysin Antibody Affinity Purified C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IMM, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-350 on human Synaptophysin protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:125

Western Blot

1:1,000

Protein

Uniprot #

P08247

Overview

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Molecular Function

Cholesterol binding, protein self-association, transporter  activity

Subcellular Location

Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein. Cell junction ›synapse › synaptosome

Expression

Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Structure

Homohexamer or homotetramer. Interacts with SRCIN1.

Alternative Nomenclature

Major synaptic vesicle protein P38 antibody

MRXSYP antibody

Syn p38 antibody

Synaptophysin antibody

SYP antibody

SYPH antibody

SypI antibody