General Information
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Product
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Phospho-p27 KIP1 Antibody
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Description
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Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody Extreme C-epitope
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Chicken, Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 148-198 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.
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Accession #
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GenBank: AAH01971.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.50-0.75 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Determinant
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Extreme C-epitope
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunohistochemistry |
1:50-1:150 |
Immunoprecipitation
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1:150
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Western Blot
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1:250
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Protein
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Uniprot #
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Q9NYG6
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Overview
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Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
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Molecular Function
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Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity
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Subcellular Location
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Nucleus
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Expression
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Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
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Structure
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Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex.
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Alternative Nomenclature
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AA408329 antibody
AI843786 antibody
CDKN 1B antibody
CDKN 4 antibody
Cyclin Dependent Kinase Inhibitor 1B antibody
Cyclin dependent kinase inhibitor p27 antibody
KIP 1 antibody
MEN1B antibody
MEN4 antibody
p27 antibody
p27 Kip1 antibody
P27-like cyclin-dependent kinase inhibitor antibody
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