General Information
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Product
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Bile Acid Receptor NR1H4 Antibody BIOTIN
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Description
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BIOTIN-Conjugated Bile acid receptor / NR1H4 / FXR A3-4 Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Huma
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Immunogen
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Synthetic peptide taken within amino acid region 1-50 on huma Bile acid receptor / NR1H4 / FXR A3-4 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5-1.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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BIOTIN-Conjugated
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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Immunoprecipitation
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1:200
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Western Blot
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1:2,500-1:5,000
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Protein
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Uniprot #
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Q96RI1
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Overview
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Ligand-activated transcription factor. Receptor for bile acids (BAs) such as chenodeoxycholic acid (CDCA), lithocholic acid, deoxycholic acid (DCA) and allocholic acid (ACA). Plays a essential role in BA homeostasis through the regulation of genes involved in BA synthesis, conjugation and enterohepatic circulation. Also regulates lipid and glucose homeostasis and is involved innate immune response (PubMed:10334992, PubMed:10334993, PubMed:21383957, PubMed:22820415).
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Molecular Function
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bile acid binding
bile acid receptor activity
chenodeoxycholic acid binding
DNA-binding transcription activator activity, RNA polymerase II-specific
DNA-binding transcription factor activity
DNA-binding transcription factor activity, RNA polymerase I
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Subcellular Location
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Nucleus
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Expression
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Liver and hepatocyte-related cells express mainly FXRalpha1-type isoforms with isoform 3 and isoform 4 in approximately equal proportions. In intestine and kidney mainly FXRalpha2-type isoforms are expressed with isoform 1 and isoform 2 in approximately equal proportions. Expressed in pancreatic beta cells and macrophages.
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Structure
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Zinc-finger
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Alternative Nomenclature
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BAR antibody
Bile acid receptor antibody
Farnesoid X activated receptor antibody
Farnesoid X receptor antibody
Farnesol receptor HRR 1 antibody
FXR antibody
HRR 1 antibody
NR1H4 antibody
Nuclear receptor subfamily 1 group H member 4 antibody
Retinoid X receptor interacting protein 14 antibodyRIP 14 antibodyRXR interacting protein 14 antibody
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