General Information
|
|
Product
|
PDE4A5 Antibody BIOTIN-Conjugated
|
Description
|
BIOTIN-Conjugated cAMP specific phosphodiesterase 4A variant pde46 Antibody
|
Verified Applications
|
CM, ELISA, ICC, IF, IHC, IP, WB
|
Host
|
Rabbit
|
Species Cross Reactivity
|
Human, Mouse, Rat
|
Immunogen
|
Synthetic cyclic peptide specific only for PDE4A5 protein. N-terminal sequence modified to achieve desired antigenicity and linker for covalent coupling to blue carrier protein.
|
Specificity |
PDE4A5 variant specific. Does not cross-react with other PDE4A proteins including: PDE4A1, PDE4A4 PDE4A8 and PDE4Ax. Does not cross-react with other PDE family members. |
Accession #
|
GenBank: EAW74861.1
|
Physical Properties
|
|
Quantity
|
100 µg
|
Volume
|
200 µl
|
Concentration
|
0.70-1.0 µg/µl in antibody stabilization buffer
|
Form
|
BIOTIN-Conjugated
|
Clonality
|
Polyclonal
|
Storage
|
-20⁰C for long term storage
|
Application Protocol
|
Confocal Microscopy
|
1:250
|
DB
|
1:10,000
|
ELISA
|
1:10,000
|
Immunocytochemistry
|
1:250
|
Immunofluorescence
|
1:250
|
Immunohistochemistry
|
1:250
|
Immunoprecipitation
|
1:250
|
Western Blot
|
1:750-1:1,000
|
Protein
|
Uniprot #
|
P27815
|
Overview
|
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
|
Molecular Function
|
Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
|
Subcellular Location
|
Isoform 1: Cytoplasm › perinuclear region, Isoform 2: Cytoplasm, Cell projection, Isoform 4: Membrane; Peripheral membrane protein Isoform 6 : Cytoplasm › perinuclear region Isoform 7 : Cytoplasm. Membrane
|
Expression
|
Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in teh heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and periphereal blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
|
Structure
|
Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.
|
Alternative Nomenclature
|
5''-cyclic phosphodiesterase 4A antibody
cAMP specific 3 5 cyclic phosphodiesterase 4A antibody
cAMP-specific 3'' antibody
DPDE2 antibody
dunce antibody
PDE46 antibody
PDE4A antibody
Phosphodiesterase 4 antibody
phosphodiesterase 4A cAMP specific antibody
phosphodiesterase E2 dunce homolog antibody
|
|
|