General Information
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Product
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PMS2 Antibody BIOTIN-Conjugated
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Description
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BIOTIN-Conjugated Mismatch Repair Endonuclease PMS2 Antibody C-epitope
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey
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Immunogen
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Synthetic peptide taken within amino acid region 620-670 on human Mismatch Repair endonuclease PMS2 protein.
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Accession #
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NCBI: XP_011513729.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.65-0.69 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Determinant
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C-epitope
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:20,000-1:50,000
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ELISA
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1:20,000-1:50,000
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Immunohistochemistry |
1:100 |
Immunoprecipitation |
1:200 |
Western Blot
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1:500-1:1,000
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Protein
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Uniprot #
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UPI0005CFF140
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Overview
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Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
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Molecular Function
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ATPase activity, ATP binding, DNA binding, endonuclease activity, single base insertion or deletion binding
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Structure
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Heterodimer of PMS2 and MLH1 (MutL alpha). Forms a ternary complex with MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3). Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MTMR15/FAN1.
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Alternative Nomenclature
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DNA mismatch repair gene homologue antibody
DNA mismatch repair protein PMS2 antibody
HNPCC4 antibody
PMS1 protein homolog 2 antibody
PMS2 antibody
PMS2CL antibody
PMSL2 antibody
Postmeiotic segregation increased, S. cerevisiae, 2 antibody
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