General Information

Product

CRMP3 Antibody

Description

Affinity Purified Dihydropyrimidinase related protein 4 Antibody

Verified Applications

ELISA, WB

Species Cross Reactivity

Cat, Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide corresponding to amino acids 509-527 of Mouse CRMP3. The peptide was covalently modified to achieve the desired antigenicity before coupling to carrier protein.

Sequence:

PAKPGSGTQARASCSGKIS

Accession #

NCBI: NP_006417.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:450

Protein

Uniprot #

O14531

Overview

Necessary for signaling by class 3 semaphorins and subsequent remodeling of cytoskeleton.  Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

Molecular Function

Axon guidance; pyrimidine nucelobase catabolic process; Nervous system development

Subcellular Location

Cytoplasm

Structure

Homotetramer.

Alternative Nomenclature

Collapsin response mediator protein 3 antibody

DPYSL4 antibody

DRP4 antibody

ULIP 4 antibody

UNC33 like phosphoprotein 4 antibody

General Information

Product

DRP1 Antibody

Description

Dynamin-related Protein 1 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

D. Melanogaster, Human, Mouse, Rat

Immunogen

Synthetic peptide within 450-490 on human DRP1 protein.

Accession #

GenBank: BAC06576.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:450

Protein

Uniprot #

Q8K1M6

Overview

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism.

Molecular Function

GTP-binding, Lipid-binding, Nucleotide-binding

Subcellular Location

Golgi apparatus; Peripheral membrane protein; peroxisome; Mitochondrion outer membrane; Clathrin-coated pit; synaptic vesicle membrane

Expression

Expressed in the cerebellum and in several regions of the cerebrum and diencephalon

Structure

Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Oligomerizes in a GTP-dependent manner to form membrane-associated tubules with a spiral pattern. Can also oligomerize to form multimeric ring-like structures.

Alternative Nomenclature

DLP1 antibody

dnm1l antibody

Dnm1p/Vps1p-like protein antibody

dnml1 antibody

DRP1 antibody

DVLP antibody

Dymple antibody

Dynamin 1 like antibody

Dynamin family member proline-rich carboxyl-terminal domain less antibody

Dynamin like protein antibody

Dynamin-like protein 4 antibody

Dynamin-related protein 1 antibody

DYNIV 11 antibody

EMPF antibody

FLJ41912 antibody

HdynIV antibody

VPS1 antibody

General Information

Product

Mitofusin 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 543-593 on human Mitofusin 2 protein.

Accession #

NCBI:  NP_055689

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunohistochemistry

1:100

Western Blot

1:500

Protein

Uniprot #

O95140

Overview

Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.

Molecular Function

Hydrolase; GTP-binding, Nucleotide binding

Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein

Expression

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

Structure

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA famil. Mitofusin subfamily. Contains 1 dynamin-type G (guanine nucleotide-binding) domain.

Alternative Nomenclature

CMT2A antibody
CMT2A2 antibody
CPRP 1 antibody
EC 3.6.5.- antibody
Fzo antibody
HSG antibody
hyperplasia suppressor gene antibody
Hypertension related protein 1 antibody
KIAA0214 antibody
MARF antibody
MFN 2 antibody

Mitochondrial assembly regulatory factor antibody
Mitofusin-2 antibody

Transmembrane GTPase MFN2 antibody

General Information

Product

TOMM40 Antibody

Description

Affinity Purified Mitochondrial import receptor subunit TOM40 homolog Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitochondrial import receptor subunit TOM40 homolog.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O96008

Overview

Channel-forming protein essential for import of protein precursors into mitochondria.

Molecular Function

Porin activity; Protein transmembrane transporter activity

Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein

Structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with mitochondrial targeting sequences.

Alternative Nomenclature

C19orf1 antibody

D19S1177E antibody

Haymaker protein antibody

Mitochondrial import receptor subunit TOM40 homolog antibody

Mitochondrial outer membrane protein TOM40 antibody

p38.5 antibody

PER EC1 antibody

Probable mitochondrial import receptor subunit TOM40 homolog antibody

Protein Haymaker antibody

TOM40 antibody

TOMM40 antibody

Translocase of outer membrane 40 kDa subunit homolog antibody

Translocase of outer mitochondrial membrane 40 homolog (yeast) antibody

General Information

Product

TOMM5 Antibody

Description

Affinity Purified Mitochondrial import receptor subunit TOM5 homolog Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Mitochondrial import receptor subunit TOM5 homolog isoform 1 protein.

Accession #

NCBI:  NP_001001790.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q8N4H5

Overview

Cellular protein metabolic process; Protein targeting to mitochondrion

Molecular Function

Protein Transport

Subcellular Location

Membrane, Mitochondrion, Mitochondrion outer membrane

Structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70).

Alternative Nomenclature

1110019J04Rik antibody

C9orf105 antibody

Mitochondrial import receptor subunit TOM5 homolog antibody

RP11-263I4.1 antibody

RP11-613M10.3 antibody

TOM5 antibody

Translocase of outer mitochondrial membrane 5 homolog (yeast) antibody

General Information

Product

TOMM6 Antibody

Description

Affinity Purified Mitochondrial import receptor subunit TOM6 homolog Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 24-74 on human Mitochondrial import receptor subunit TOM6 homolog.

Accession #

NCBI:  NP_001127965.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96B49

Overview

Cellular protein metabolic process; Protein targeting to mitochondrion

Molecular Function

Protein Transport

Subcellular Location

Membrane, Mitochondrion, Mitochondrion outer membrane

Structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70).

Alternative Nomenclature

Mitochondrial import receptor subunit TOM6 homolog antibody
TOM6 antibody

General Information

Product

TOMM7 Antibody

Description

Affinity Purified Mitochondrial import receptor subunit TOM7 homolog Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Pig, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Mitochondrial import receptor subunit TOM7 homolog protein.

Accession #

NCBI:  NP_061932.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9P0U1

Overview

Required for assembly and stability of the TOM complex. Positive regulator of PARK2 translocation to damaged mitochondria. Acts probably by stabilizing PINK1 on the outer membrane of depolarized mitochondria.

Molecular Function

Cellular protein metabolic process; Protein targeting to mitochondrion

Subcellular Location

Membrane, Mitochondrion, Mitochondrion outer membrane

Structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70).

Alternative Nomenclature

Mitochondrial import receptor subunit TOM7 homolog antibody
TOM7 antibody

General Information

Product

ATG16L1 Antibody

Description

Affinity Purified Autophagy-related protein 16-1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 560-610 on human Autophagy-related protein 16-1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q676U5

Overview

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.

Molecular Function

Identical protein binding, Ubiquitin-like protein transferase activity

Subcellular Location

Cytoplasm, Preautophagosomal structure membrane, Peripheral membrane protein

Structure

Homooligomer. Interacts with WIPI2, FIP200 and ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B. Interacts with TMEM

Alternative Nomenclature

APG16 like 1 antibody

APG16L antibody

APG16L beta antibody

ATG16 autophagy related 16 like 1 antibody

ATG16A antibody

ATG16L antibody

Autophagy related protein 16 1 antibody

IBD10 antibody

WD repeat domain 30 antibody

WDR30 antibody

General Information

Product

ULK1 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase ULK1 Antibody

Verified Applications

ELISA, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Panda

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on mouse ULK1 protein.

Accession #

NCBI:  NP_003556.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

O75385

Overview

Involved in autophagy in response to starvation. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Molecular Function

ATP Binding, protein serine/threonine kinase activity, Rab GTPase binding, protein complex binding

Subcellular Location

Cytoplasm, cytosol, preautphagosomal structure. (Note: under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome)

Expression

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Alternative Nomenclature

ATG1 autophagy related 1 homolog antibody

ATG1A antibody

Autophagy related protein 1 homolog antibody

hATG1 antibody

Serine/threonine-protein kinase ULK1 antibody

ULK1 antibody

Unc 51 like kinase 1 antibody

Unc-51 like kinase 1 (C. elegans) antibody

UNC51 antibody

General Information

Product

AKT1 Antibody

Description

Affinity Purified RAC-alpha serine/threonine-protein kinase Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within the last 50 amino acid on human RAC-alpha serine/threonine-protein kinase protein.

Accession #

GeneBank: AAA36539

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P31749

Overview

One of 3 serine/threonine-protein kinases (AKT1-AKT3) regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

Molecular Function

Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in prostate cancer and all human cell types.

Structure

Interacts with and phosphorylated by PDPK1.  Interacts with several proteins invovled in phosphorylation and ubiquitination

Alternative Nomenclature

PKB antibody

Protein Kinase B Alpha antibody

RAC Serine/Threonine Protein Kinase antibody

vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody