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General Information
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Product
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HIPK1 Antibody
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Description
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Homeodomain-interacting protein kinase 1 Antibody
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Verified Applications
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ELISA, IP, WB
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 700-750 on human Homeodomain-interacting protein kinase 1 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5-1.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Purification Method
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Immobilized antigen affinity chromatography
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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Immunoprecipitation
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1:200
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Western Blot
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1:2,500-1:5,000
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Protein
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Uniprot #
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Q86Z02
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Overview
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Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation.
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Molecular Function
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ATP binding
protein serine/threonine kinase activity
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Subcellular Location
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Nucleus
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Expression
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Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis.
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Structure
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Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
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Alternative Nomenclature
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Hipk 1 antibody
Homeodomain interacting protein kinase 1 antibody
Myak antibody
Nbak 2 antibody
Nuclear body associated kinase 2 antibody
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HIPK1 Antibody BIOTIN
HIPK1 Antibody FITC
HIPK1 Blocking Peptide
HIPK1 Positive Control
