General Information
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Product
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Phantom Antibody
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Description
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Affinity Purified Phantom isoform A Antibody Mid-Region
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Verified Applications
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CM, ELISA, ICC, IF, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Drosophila melanogaster
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Immunogen
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Mid region epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Phantom protein.
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Accession #
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NCBI: NP_573319.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.75 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Determinant
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Mid-Region
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Storage
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-20⁰C for long term storage
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Application Protocol
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Confocal Microscopy
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1:200
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DB
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1:10,000
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ELISA
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1:10,000
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Immunocytochemistry
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1:200
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Immunofluorescence
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1:200
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Immunohistochemistry
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1:200
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Immunoprecipitation
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1:200
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Western Blot
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1:500
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Protein
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Uniprot #
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Q9VWR5
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Overview
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Plays a role in the metabolism of insect hormones through ecdysteroid C25-hydroxylase activity. May also funciton in the breakdown of synthetic insecticides.
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Molecular Function
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Monooxygenase, Oxireductase
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Subcellular Location
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Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane
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Expression
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First detected at early blastoderm stage 4, and stripes of expression remain through step 10. Expression is not detectable during germ band retraction and some expression resumes during stae 15-17. Low levels of expression are detected in larval brain a
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Alternative Nomenclature
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Cytochrome P450 306a1 antibody
Dmphm antibody
Exo antibody
EXO1 antibody
Exocephalon antibody
Protein phantom antibody
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