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General Information
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Product
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OGT1 Peptide
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Description
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Antigenic Blocking Peptide O-linked N-acetylglucosamine transferase Antibody
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Verified Applications
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Immunodepletion, Immunocompetition
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Peptide Description
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Synthetic peptide taken within amino acid region 980-1030 on human O-linked N-acetylglucosamine transferase protein.
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Physical Properties
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Quantity
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250 µg
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Volume
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100 µl
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Form
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Antigenic Blocking Peptide
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Storage
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-20⁰C for long term storage
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Application Protocol
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Competition/Depletion assay
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Refer to competition assay protocol
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Protein
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Uniprot #
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O15294
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Overview
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Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity.
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Molecular Function
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Chromatin regulator, Glycosyltransferase, Transferase
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Subcellular Location
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Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus
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Expression
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Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
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Structure
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The TPR repeat domain is required for substrate binding and oligomerization.
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Alternative Nomenclature
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GlcNAc transferase
HRNT1
O GlcNAc
O GlcNAc transferase p110 subunit
O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase)
UDP N acetylglucosamine peptide N
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
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OGT1 Antibody
OGT1 Antibody BIOTIN
OGT1 Antibody FITC
OGT1 Positive Control
