General Information

Product

PDE4A5 Antibody

Description

Affinity Purified cAMP specific phosphodiesterase 4A variant pde46 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic cyclic peptide specific only for PDE4A5 protein. N-terminal sequence modified to achieve desired antigenicity and linker for covalent coupling to blue carrier protein.

Accession #

GenBank: EAW74861.1 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:750-1:1,000

Protein

Uniprot #

P27815

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Isoform 1: Cytoplasm › perinuclear region, Isoform 2: Cytoplasm, Cell projection, Isoform 4: Membrane; Peripheral membrane protein Isoform 6 : Cytoplasm › perinuclear region Isoform 7 : Cytoplasm. Membrane

Expression

Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in teh heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and periphereal blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).

Structure

Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.

Alternative Nomenclature

5''-cyclic phosphodiesterase 4A antibody

cAMP specific 3 5 cyclic phosphodiesterase 4A antibody

cAMP-specific 3'' antibody

DPDE2 antibody

dunce antibody

PDE46 antibody

PDE4A antibody

Phosphodiesterase 4 antibody

phosphodiesterase 4A cAMP specific antibody

phosphodiesterase E2 dunce homolog antibody

General Information

Product

PDE4B1 Antibody

Description

Affinity Purified cAMP-specific phosphodiesterase isoform PDE4B1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide selective only for PDE4B1 subtype variant.

Accession #

GenBank: AAL31763.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.90 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P14646

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Expression

Widely expressed. Isoform 3 expressed in brain, heart, lung and liver. Isoform 4 expressed in liver and brain.

Alternative Nomenclature

5''-cyclic phosphodiesterase 4B antibody

cAMP-specific 3'' antibody

cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody

cAMP-specific phosphodiesterase-4 B isoform antibody

DKFZp686F2182 antibody

DPDE4 antibody

dunce-like phosphodiesterase E4 antibody

MGC126529 antibody

PDE 4B antibody

PDE IVB antibody

PDE32 antibody

PDE4B5 antibody

Phosphodiesterase 4B antibody

Phosphodiesterase 4B cAMP specific antibody

General Information

Product

PDE4B2 Antibody

Description

Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human PDE4B2 protein.

Accession #

NCBI:  NP_001032416.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:750

Protein

Uniprot #

Q07343

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Expression

Expressed in brain, heart, lung and skeletal muscle.

Alternative Nomenclature

5''-cyclic phosphodiesterase 4B antibody

cAMP-specific 3'' antibody

DPDE4 antibody

dunce-like phosphodiesterase E4 antibody

MGC126529 antibody

PDE 4B antibody

PDE IVB antibody

PDE32 antibody

PDE4B5 antibody

PDEIVB antibody

Phosphodiesterase-4B2 cAMP-specific antibody

General Information

Product

PDE4B3 Antibody

Description

Affinity Purified cAMP-specific phosphodiesterase PDE4B Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide selective only for PDE4B3 subtype.

Accession #

GenBank: AAB96560.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P14646

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Expression

Widely expressed. Isoform 3 expressed in brain, heart, lung and liver. Isoform 4 expressed in liver and brain.

Alternative Nomenclature

DPDE4 antibody

PDE IVB antibody

Phosphodiesterase 4B antibody

Phosphodiesterase Type 4B3 antibody

General Information

Product

PDE4B4 Antibody

Description

Affinity Purified cAMP-specific phosphodiesterase isoform PDE4B4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide selective only for PDE4B4 protein.

Accession #

GenBank: AAL31764.1 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250-1:500

Protein

Uniprot #

P14646

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Expression

Widely expressed. Isoform 3 expressed in brain, heart, lung and liver. Isoform 4 expressed in liver and brain.

Alternative Nomenclature

cAMP dependent phosphodiesterase type B antibody

cAMP specific 3 5 cyclic phosphodiesterase 4B antibody

CAMP specific phosphodiesterase isoform PDE4B4 antibody

DPDE4 antibody

Dunce like phosphodiesterase E4 antibody

MGC126529 antibody

PDE4B 4 antibody

PDEIVB antibody

Phosphodiesterase 4B cAMP specific antibody

Phosphodiesterase E4 dunce homolog antibody

Phosphodiesterase E4 dunce homolog Drosophila antibody

General Information

Product

PDE4D Antibody

Description

Affinity Purified Phosphodiesterase 4D Antibody

Verified Applications

ELISA, IP, ICC/IF, IHC-P, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-terminal cyclic peptide unique to all PDED subtypes variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).

Alternative Nomenclature

cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

DKFZp686M11213 antibody

5''-cyclic phosphodiesterase 4D antibody

cAMP specific phosphodiesterase PDE4D antibody

cAMP specific phosphodiesterase PDE4D6 antibody

DPDE 3 antibody

Dunce like phosphodiesterase E3 antibody

FLJ97311 antibody

HSPDE4D antibody

PDE 43 antibody

PDE4DN2 antibody

Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody

Drosophila antibody

STRK1 antibody

General Information

Product

PDE11A Antibody

Description

Affinity Purified Cyclic Nucleotide Phosphodiesterase 11A1 Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on PDE11A protein, common to all PDE11A variants.

Accession #

GenBank: CAB82573.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q9HCR9

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.

Molecular Function

Hydrolase, metal-ion binding, cGMP binding

Subcellular Location

Cytoplasm

Expression

Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed i

Alternative Nomenclature

5''-cyclic-AMP and -GMP phosphodiesterase 11A antibody

cAMP and cGMP cyclic nucleotide phosphodiesterase 11A antibody

Cyclic nucleotide phosphodiesterase 11A1 antibody

Dual 3' 5' cyclic AMP and GMP phosphodiesterase 11A antibody

FLJ23693 antibody

MGC133355 antibody

MGC133356 antibody

PDE 11A antibody

pde11 antibody

pde11a2 antibody

Phosphodiesterase 11A antibody

PPNAD2 antibody

General Information

Product

PDE11A Antibody

Description

Affinity Purified Cyclic Nucleotide Phosphodiesterase 11A1 Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 454-468 of human PDE11A.

Accession #

GenBank: CAB82573.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q9HCR9

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.

Molecular Function

Hydrolase, metal-ion binding, cGMP binding

Subcellular Location

Cytoplasm

Expression

Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil is not relevant (at protein level). Isoform 2 may be expressed in testis.

Alternative Nomenclature

5''-cyclic-AMP and -GMP phosphodiesterase 11A 

cAMP and cGMP cyclic nucleotide phosphodiesterase 11A 

Cyclic nucleotide phosphodiesterase 11A1 

Dual 3' 5' cyclic AMP and GMP phosphodiesterase 11A 

PDE 11A 

pde11 

pde11a2 

Phosphodiesterase 11A 

PPNAD2 

General Information

Product

PDE3A Antibody

Description

Affinity Purified cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 1 Antibody

Verified Applications

ELISA, FRET, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat, Xenopus

Immunogen

Synthetic peptide selective only for PDE3A variants.

Accession #

NCBI:  NP_000912.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.719 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q14432

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.

Molecular Function

Hydrolase, 3', 5'-cyclic-GAP phosphodiesterase activity, cAMP binding

Subcellular Location

Membrane; Multi-pass membrane protein

Alternative Nomenclature

cAMP phosphodiesterase myocardial cGMP inhibited antibody

CGI PDE A antibody

CGI PDE antibody

Cyclic GMP-inhibited phosphodiesterase A antibody

Human cGMP inhibited cAMP phosphodiesterase mRNA antibody

PDE 3A antibody

Phosphodiesterase 3A antibody

Phosphodiesterase 3A cGMP inhibited antibody

General Information

Product

PDE4A1 Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide specific for PDE4A1 protein.

Accession #

GenBank: L27062.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P54748

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Cytoplasm >  perinuclear region

Expression

Isoform 2 is testis specific.

Structure

Interacts with LYN and ARRB2.

Alternative Nomenclature

5''-cyclic phosphodiesterase 4A antibody

cAMP specific 3 5 cyclic phosphodiesterase 4A antibody

cAMP-specific 3'' antibody

DPDE2 antibody

dunce like phosphodiesterase E2 antibody

PDE 4A antibody

PDE4 antibody

PDE46 antibody

PDE4A11 antibody

phosphodiesterase E2 dunce homolog antibody

phosphodiesterase isozyme 4 antibody

phosphodiesterase type 4A11 antibody