General Information
|
|
Product
|
Ki67 Antibody
|
Description
|
Affinity Purified Antigen KI-67 isoform 1 Antibody N-epitope
|
Verified Applications
|
ELISA, IHC, IP, WB
|
Host
|
Rabbit
|
Species Cross Reactivity
|
Human, Monkey
|
Immunogen
|
Synthetic peptide taken within amino acid region 1250-1300 on human antigen Ki67 isoform 1.
|
Specificity |
This antibody will detect both isoform 1 and isoform 2 in human. |
Accession #
|
NCBI: NP_002408.3
|
Physical Properties
|
|
Quantity
|
100 µg
|
Volume
|
200 µl
|
Concentration
|
0.55-0.75 µg/µl in antibody stabilization buffer
|
Form
|
Affinity Purified
|
Clonality
|
Polyclonal
|
Determinant
|
Mid-region epitope
|
Storage
|
-20⁰C for long term storage
|
Application Protocol
|
DB
|
1:10,000
|
ELISA
|
1:10,000
|
Immunohistochemistry |
1:50-1:150 |
Immunoprecipitation |
1:200 |
Western Blot
|
1:500
|
Protein
|
Uniprot #
|
P46013
|
Overview
|
required for maintaining cell proliferation
|
Molecular Function
|
poly(A) RNA binding; protein C-terminus binding; ATP binding
|
Subcellular Location
|
Chromosome, Nucleus
|
Expression
|
Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
|
Alternative Nomenclature
|
Antigen identified by monoclonal antibody Ki 67 antibody
KIA antibody
MKI67 antibody
Proliferation related Ki 67 antigen antibody
RP11-380J17.2 antibody
|
|
|