General Information |
|
Product |
PDE1C Antibody |
Description |
Affinity Purified Human 3',5' cyclic nucleotide phosphodiesterase (HSPDE1C1A) Antibody N-epitope |
Verified Applications |
CM, ELISA, ICC, IF, IHC, IP, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Mouse, Rat |
Immunogen |
Synthetic peptide corresponding to unique amino acid sequence on PDE1C gene. The sequence was taken within amino acid region 1-50. |
Specificity | This antibody does not cross-react with PDE1A, PDE1B, or other PDE family members. This antibody will label all PDE1C variants including PDE1C1, PDE1C2, and PDE1C3. |
Accession # |
GenBank: U40371.1 |
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|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.5 µg/µl in antibody stabilization buffer |
Form |
Affinity Purified |
Clonality |
Polyclonal |
Determinant |
N-epitope |
Storage |
-20⁰C for long term storage |
|
|
Confocal Microscopy |
1:40-1:100 |
DB |
1:10,000 |
ELISA |
1:10,000 |
Immunocytochemistry |
1:40-1:100 |
Immunofluorescence |
1:100 |
Immunohistochemistry |
1:40-1:100 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500-1:2,000 |
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|
Uniprot # |
|
Overview |
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP. |
Molecular Function |
Hydrolase, calmodulin-dependent cyclic-nucleotide phosphodiesterase activity, metal ion binding |
Subcellular Location |
Cytosol |
Expression |
Expressed in several tissues, including brain and heart. |
Structure |
Homodimer |
Alternative Nomenclature |
5''-cyclic nucleotide phosphodiesterase 1C Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C Cam PDE 1C HCAM3 HSPDE1C1A Human 3'5' cyclic nucleotide Phosphodiesterase PDE 1C Phosphodiesterase 1C Phosphodiesterase 1C calmodulin dependent |
Protein information supplied by UniProt
FabGennix PDE1C Antibody has been used in 16 Publications
Applications: Confocal microscopy, Immunodepletion, Immunofluorescence, Immunohistochemistry, and Western blot
Reactivity: Chicken, Human, Mouse, and Rat
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Cesarini, Valeriana, et al. "Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome." Oncotarget (2017).
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PMID: 28099939 Applications: Western Blot Reactivity: Human |
Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015).
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PMID: 25578602 Applications: Immunohistochemistry Reactivity: Human |
Yougbaré, Issaka, et al. "Cyclic GMP catabolism up-regulation in MRL/lpr lupus-prone mice is associated with organ remodeling." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1842.7 (2014).
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PMID: 24631654 Applications: Immunohistochemistry, Western Blot Reactivity: Mouse |
Guibinga, Ghiabe-Henri, Fiona Murray, and Nikki Barron. “HPRT-Deficiency Dysregulates cAMP-PKA Signaling and Phosphodiesterase 10A Expression: Mechanistic Insight and Potential Target for Lesch-Nyhan Disease?” Ed. Kjetil Tasken. PLoS ONE 8.5 (2013).
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PMCID: 3653951 Applications: Western Blot Reactivity: Human |
Genders, Amanda J., et al. "cGMP phosphodiesterase inhibition improves the vascular and metabolic actions of insulin in skeletal muscle." American Journal of Physiology-Endocrinology and Metabolism 301.2 (2011).
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PMID: 21653225 Applications: Immunofluorescence, Western Blot Reactivity: Mouse |
Abusnina, Abdurazzag, et al. "Down-regulation of cyclic nucleotide phosphodiesterase PDE1A is the key event of p73 and UHRF1 deregulation in thymoquinone-induced acute lymphoblastic leukemia cell apoptosis." Cellular signalling 23.1 (2011).
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PMID: 20807569 Applications: Western Blot Reactivity: Human |
Abusnina, Abdurazzag, et al. "Anti‐proliferative effect of curcumin on melanoma cells is mediated by PDE1A inhibition that regulates the epigenetic integrator UHRF1." Molecular nutrition & food research 55.11 (2011).
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PMID: 22045655 Applications: Western Blot Reactivity: Human |
Dong, Hongli, et al. "Inhibition of PDE3, PDE4 and PDE7 potentiates glucocorticoid-induced apoptosis and overcomes glucocorticoid resistance in CEM T leukemic cells." Biochemical pharmacology 79.3 (2010).
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PMID: 19737543 Applications: Western Blot Reactivity: Human |
Ortiz-Capisano, M. Cecilia et al. “Calcium-Dependent Phosphodiesterase 1C Inhibits Renin Release from Isolated Juxtaglomerular Cells.” American Journal of Physiology - Regulatory, IntegRative and CompaRative Physiology 297.5 (2009).
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PMCID: 2777770 Applications: Confocal microscopy, Immunohistochemistry, Immunofluorescence, Western Blot Reactivity: Mouse |
Tahseldar-Roumieh, Rima et al. “PDEs1-5 Activity and Expression in Tissues of Cirrhotic Rats Reveal a Role for Aortic PDE3 in NO Desensitization.” International Journal of Experimental Pathology 90.6 (2009).
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PMCID: 2803251 Applications: Western Blot Reactivity: Rat |
Deplano, Stefania, et al. "Gene expression and protein localization of calmodulin‐dependent phosphodiesterase during ontogenesis of chick retina." Journal of neuroscience research 86.5 (2008).
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PMID: 18041092 Applications: Immunohistochemistry, Western Blot Reactivity: Chicken |
Dunkern, Torsten R., and Armin Hatzelmann. "Characterization Of Inhibitors Of Phosphodiesterase 1C On A Human Cellular System." FEBS Journal 274.18 (2007).
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PMID: 17697115 Applications: Western Blot Reactivity: Human |
Murray, Fiona, et al. "Expression and activity of cAMP phosphodiesterase isoforms in pulmonary artery smooth muscle cells from patients with pulmonary hypertension: role for PDE1." American Journal of Physiology-Lung Cellular and Molecular Physiology 292.1 (2007).
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PMID: 16980375 Applications: Western Blot Reactivity: Human |
Santone, Rocco, et al. "Gene expression and protein localization of calmodulin‐dependent phosphodiesterase in adult Rat retina." Journal of neuroscience research 84.5 (2006).
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PMID: 16881052 Applications: Confocal microscopy, Immunohistochemistry, Western Blot Reactivity: Rat |
Dolci, Susanna, et al. "Subcellular localization and regulation of type-1C and type-5 phosphodiesterases." Biochemical and biophysical research communications 341.3 (2006).
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PMID: 16455054 Applications: Immunodepletion, Immunofluorescence, Western Blot Reactivity: Human |
Baxendale, Rhona W., and Lynn R. Fraser. "Mammalian sperm phosphodiesterases and their involvement in receptor‐mediated cell signaling important for capacitation." Molecular reproduction and development 71.4 (2005).
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PMID: 15856425 Applications: Immunofluorescence, Western Blot Reactivity: Mouse |
Publishing research using PD1C-301AP? Please let us know, we can cite the reference in this datasheet.
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