TNIK Antibody

General Information

Product

TNIK Antibody

Description

Affinity Purified TRAF2 and NCK-interacting protein kinase isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Sythetic peptide taken within amino acid region 1250-1300 on human TRAF2 and NCK-interacting protein kinase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9UKE5

Overview

Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.

Molecular Function

ATP binding

MAP kinase kinase kinase kinase activity

protein kinase activity

protein serine/threonine kinase activity

Subcellular Location

Nucleus, Cytoskeleton, Endosome

Expression

Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Alternative Nomenclature

EC 2.7.11.1 antibody

KIAA0551 antibody

Tnik antibody

TNIK_HUMAN antibody

Traf2 and NCK interacting kinase antibody