5HT2A Receptor Antibody

General Information

Product

5HT2A Receptor Antibody

Description

Affinity Purified 5-hydroxytryptamine receptor 2A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human 5-hydroxytryptamine receptor 2A.

Accession #

NCBI:  NP_000612

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunocytochemistry

1:50-1:100

Immunohistochemistry

1:50-1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P28223

Overview

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.

Molecular Function

G-protein coupled receptor, Host cell receptor for virus entry, Receptor, Transducer

Subcellular Location

Cell Membrane, Cell projection

Expression

Detected in brain cortex. Detected in blood platelets.

Structure

The PDZ domain-binding motif is involved in the interaction with PATJ, CASK, APBA1, DLG1 and DLG4.

Alternative Nomenclature

5 HT2 receptor 

5 HT2A 

5 hydroxytryptamine receptor 2A 

5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled 

HTR 2A 

HTR2 

serotonin 5-HT-2A receptor 

Serotonin receptor 2A