Your datasheets states this antibody was raised against "aa 1-70 of ACTH." However, ACTH (AKA corticotropin) is only a 38 aa protein (aa 138-176 of POMC) that is formed from cleavage of POMC, see the uniprot file below. I wanted to confirm if this antibody was raised against amino acids 1-70 or 138-176 of POMC.http://www.uniprot.org/uniprot/P01189
The ACTH antibodies were raised against 39 amino acid ACTH hormone. The Specification sheet specifies the range from where the peptide was selected. In this case the 39 AA are the complete ACTH hormone.
ACTH human 1-39 SYS MEH FRW GKP VGK KRR PVK VYP NGA EDE SAE AFP LEF
What is the percentage of homology between the immune source sequence of this antibody and the dog sequence?
Thank you for your email inquiry for the homology of our product ACTH-101AP with dog sequence. The antigen used for generating our ACTH antibody is 99.00% homologous to dog (Canis Lupus familiaris) ACTH sequence except for a single conservative substitution of Leucine to valine near the C-terminal end. OUr antibody wil label and detect dog ACTH peptide.
Customer ratings for ACTH Antibody
Number of ratings: 1
Average rating: 5
Excellent from Anonymous on 11/13/2015
Western Blot Pig Brain purified protein Reduced Denaturing (4-12% Bis-Tris) 10 ug sample loaded BSA used as blocking agent for 1 hour. 3% at 22C. 1:500 primary antibody dilution for 1 hour at 22C. DCIP/NBT detection method Bands: Specific: 5, 10, 15, 20 kDa.
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