Your datasheets states this antibody was raised against "aa 1-70 of ACTH." However, ACTH (AKA corticotropin) is only a 38 aa protein (aa 138-176 of POMC) that is formed from cleavage of POMC, see the uniprot file below. I wanted to confirm if this antibody was raised against amino acids 1-70 or 138-176 of POMC.
The ACTH antibodies were raised against 39 amino acid ACTH hormone. The Specification sheet specifies the rangefrom where the peptide was selected. In this case the 39 AA are the complete ACTH hormone.
SYS MEH FRW GKP VGK KRR PVK VYP NGA EDE SAE AFP LEF
The peptide was conjugated with a carrier protein for making ACTH antibodies.
Customer ratings for ACTH Antibody
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Excellent from Anonymous on 11/13/2015
Western Blot Pig Brain purified protein Reduced Denaturing (4-12% Bis-Tris) 10 ug sample loaded BSA used as blocking agent for 1 hour. 3% at 22C. 1:500 primary antibody dilution for 1 hour at 22C. DCIP/NBT detection method Bands: Specific: 5, 10, 15, 20 kDa.