DWORF Antibody

General Information

Product

DWORF Antibody

Description

Affinity Purified Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-30 on mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P0DN83

Overview

Enhances the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by displacing ATP2A1/SERCA1 inhibitors, thereby acting as a key regulator of skeletal muscle activity. Does not directly stimulates SERCA pump activity. Enhances sarcoplasmic reticulum Ca2+ uptake and myocyte contractility by displacing the SERCA inhibitory peptides sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN).

Subcellular Location

Sarcoplasmic reticulum membrane; Single-pass membrane protein

Expression

Highly expressed in heart (at protein level). Detected in heart and soleus, a postural muscle group of the hindlimb containing the highest enrichment of slow-twitch muscle fibers. Also expressed in diaphragm, which contains some slow-twitch fibers. Not detected in the quadriceps, a fast-twitch muscle group, or in cardiac atrial muscle. Not expressed in the prenatal heart but gradually increases in abundance postnatally.

Alternative Nomenclature

Dwarf open reading frame antibody

DWORF antibody

Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF

SERCA regulator DWORF antibody