FGFR3 Antibody

Product no.: FGFR3-301AP

$285.00

Description

General Information
Product	FGFR3 Antibody
Description	Affinity Purified Fibroblast Growth Factor Receptor 3 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey
Immunogen	Synthetic peptide taken within amino acid region 400-450 on human FGFR3 protein.
Accession #	NCBI: XP_011537812.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55-0.75 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunohistochemistry	1:50-1:100
Immunoprecipitation	1:150
Western Blot	1:500
Protein
Uniprot #	A8E633
Overview	Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
Molecular Function	ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity
Subcellular Location	Isoform 1 : Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Endoplasmic reticulum Note: The activated receptor is rapidly internalized and degraded. Detected in intracellular vesicles after internalization of the autophosphorylated receptor. Isoform 2 : Cell membrane By similarity; Single-pass type I membrane protein By similarity Isoform 3 : Secreted Isoform 4 : Cell membrane By similarity; Single-pass type I membrane protein
Expression	Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Isoform 2 is detected in epithelial cells. Isoform 1 is not detected in epithelial cells. Isoform 1 and isoform 2 are detected in fibroblastic cells.
Structure	Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Alternative Nomenclature	ACH antibody CD 333 antibody CD333 antigen antibody CEK 2 antibody FGFR 3 antibody Fibroblast growth factor receptor 3 (achondroplasia thanatophoric dwarfism) antibody Fibroblast growth factor receptor 3 antibody Heparin binding growth factor receptor antibody HSFGFR3EX antibody Hydroxyaryl protein kinase antibody JTK 4 antibody MFR 3 antibody SAM 3 antibody Tyrosine kinase JTK 4 antibody Z FGFR 3 antibody

Protein information supplied by UniProt