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General Information
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Product
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Legumain Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 100-150 on human Legumain protein.
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Accession #
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NCBI: XP_016876954
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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Affinity Purified
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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| Immunohistochemistry |
1:50-1:150 |
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Immunoprecipitation
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1:200
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Western Blot
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1:500
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Protein
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Uniprot #
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Q99538
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Overview
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Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
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Subcellular Location
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Lysosome
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Expression
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Ubiquitous. Particularly abundant in kidney, heart and placenta.
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Structure
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In the zymogen form, the uncleaved propeptide blocks access to the active site.
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Alternative Nomenclature
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AEP antibody
Asparaginyl endopeptidase antibody
cysteine 1 antibody
Cysteine protease 1 antibody
Legumain antibody
LGMN antibody
LGMN1 antibody
Protease antibody
Protease cysteine 1 (legumain) antibody
Protease cysteine 1 antibody
PRSC1 antibody
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Legumain Antibody BIOTIN
Legumain Antibody FITC
Legumain Blocking Peptide
Legumain Positive Control
