Legumain Antibody

Product no.: LGMN-101AP

$285.00

Description
Publications

General Information
Product	Legumain Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 100-150 on human Legumain protein.
Accession #	NCBI: XP_016876954
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Purification Method	Immobilized antigen affinity chromatography
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:50,000
Immunohistochemistry	1:50-1:150
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q99538
Overview	Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Subcellular Location	Lysosome
Expression	Ubiquitous. Particularly abundant in kidney, heart and placenta.
Structure	In the zymogen form, the uncleaved propeptide blocks access to the active site.
Alternative Nomenclature	AEP antibody Asparaginyl endopeptidase antibody cysteine 1 antibody Cysteine protease 1 antibody Legumain antibody LGMN antibody LGMN1 antibody Protease antibody Protease cysteine 1 (legumain) antibody Protease cysteine 1 antibody PRSC1 antibody

Protein information supplied by UniProt