General Information
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Product
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MAPK14 Antibody
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Description
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Affinity Purified p38 Mitogen Activated Protein Kinase 14 Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 150-200 on human Mitogen Activated Protein Kinase 14 protein.
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Accession #
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GenBank: ACI00233.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.65-0.69 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:20,000-1:50,000
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ELISA
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1:20,000-1:50,000
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Immunohistochemistry |
1:100 |
Immunoprecipitation |
1:200 |
Western Blot
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1:500-1:1,000
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Protein
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Uniprot #
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Q16539
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Overview
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Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation.
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Molecular Function
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ATP binding; MAP kinase activity; MAP kinase kinase activity; NFAT protein binding; Protein serine/threonine kinase activity
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Subcellular Location
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Cytoplasm; Nucleus
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Structure
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Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B.
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Alternative Nomenclature
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CSAID-binding protein antibody
CSBP antibody
Cytokine suppressive anti-inflammatory drug-binding protein antibody
EXIP antibody
MAP kinase 14 antibody
MAP kinase p38 alpha antibody
MAPK14 antibody
MAX-interacting protein 2 antibody
Mitogen-activated protein kinase 14 antibody
p38 antibody
p38 MAP kinase antibody
SAPK2A antibody
Stress-activated protein kinase 2a antibody
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