Nkx2.2 Antibody

General Information

Product

Nkx2.2 Antibody Affinity Purified

Description

Affinity Purified homeobox protein Nkx-2.2 Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 200-273 on human homeobox protein Nkx-2.2

Accession #

NCBI:  NP_002500

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O95096

Overview

Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity).

Molecular Function

Chromatin binding; transcription coactivator activity; Developmental Protein; Transcription regulation; brain development

Subcellular Location

Nucleus

Expression

Interacts with OLIG2

Structure

Belongs to the NK-2 homeobox family.  Contains 1 homeobox DNA-binding domain.  The homeodomain is essential for interaction with OLIG2.

Alternative Nomenclature

Homeobox protein NK 2 homolog B antibody

NK 2 homolog B antibody

NK2 homeobox 2 antibody

NK2 transcription factor like protein B antibody

NK2 transcription factor related locus 2 antibody

NKX2-2 antibody

NKX22 antibody

Nkx2b antibody

tinman antibody