CARD15 Antibody

Product no.: NOD2-201AP

$285.00

Description

General Information
Product	CARD15 Antibody
Description	Affinity Purified Nucleotide-binding oligomerization domain-containing protein 2
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 700-780 on mouse Nucleotide-binding oligomerization domain-containing protein 2.
Accession #	GenBank: AAH44774
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5-1.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Purification Method	Immobilized antigen affinity chromatography
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:50,000
Immunohistochemistry	1:50-1:150
Immunoprecipitation	1:200
Western Blot	1:2,500-1:5,000
Protein
Uniprot #	Q8K3Z0
Overview	Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response (PubMed:22607974). Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561).
Molecular Function	actin binding ATP binding CARD domain binding endodeoxyribonuclease activity, producing 5'-phosphomonoesters enzyme binding Hsp70 protein binding Hsp90 protein binding muramyl dipeptide binding peptidoglycan binding protein kinase binding
Subcellular Location	Cytoplasm Membrane Basolateral cell membrane
Structure	The ATG16L1-binding motif mediates interaction with ATG16L1. Intramolecular interactions between the N-terminal moiety and the leucine-rich repeats (LRR) may be important for autoinhibition in the absence of activating signal. In the absence of LRRs, the protein becomes a constitutive activator of CASP1 cleavage and proIL1B processing.
Alternative Nomenclature	ACUG antibody Arthrocutaneouveal granulomatosis antibody BLAU antibody CARD15 antibody Caspase recruitment domain protein 15 antibody CD antibody CLR16.3 antibody IBD1 antibody Inflammatory bowel disease protein 1 antibody LRR containing protein antibody NLR family, CARD domain containing 2 antibody NLRC2 antibody NOD like receptor C2 antibody NOD2 antibody NOD2B antibody Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2 antibody PSORAS1 antibody

Protein information supplied by UniProt.