Insulin Receptor Positive Control

General Information

Product

Insulin Receptor Positive Control

Description

Western Blot Positive Control Insulin receptor alpha and beta

Verified Applications

WB

Immunogen

Purified Protein

Accession #

NCBI:  NM_001079817.1

Physical Properties

Quantity

5 Applications

Volume

Inquire

Form

Western Blot Positive Control

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

P06213

Overview

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.

Structure

Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds.

Alternative Nomenclature

CD220 antibody

HHF5 antibody

HIR A antibody

INSR alpha antibody

Insulin receptor antibody

Insulin receptor subunit alpha antibody

IR antibody

HHF5 antibody

human insulin receptor antibody

Insulin receptor subunit beta antibody

IR 1 antibody