MYLK Positive Control

General Information

Product

MYLK Positive Control

Description

Western Blot Positive Control Myosin light chain kinase, smooth muscle isoform 1

Verified Applications

WB

Immunogen

Purified Protein

Accession #

NCBI:  NP_444253.3

Physical Properties

Quantity

5 Applications

Volume

Inquire

Form

Western Blot Positive Control

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q15746

Overview

Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Cell projection > lamellipodium, Cleavage furrow, Cytoplasm > cytoskeleton

Expression

Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).

Structure

All isoforms including Telokin bind calmodulin. Interacts with SVIL. Interacts with CTTN; this interaction is reduced during thrombin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Binds to NAA10/ARD1 and PTK2B/PYK2.

Alternative Nomenclature

caMLCK antibody

cardiac MLCK antibody

Cardiac-MyBP-C-associated Ca/CaM kinase antibody

MLC kinase antibody

MLCK antibody

MLCK2 antibody

Mylk3 antibody

Myosin light chain kinase 3 antibody

Putative myosin light chain kinase 3 antibody